A possible method for the detection of ovulation and the determination of the duration of the fertile period

Baker, Terence S.; Jennison, K M; Kellie, A. E.

doi:10.1016/0022-4731(80)90300-3

Cited by 26 publications

(8 citation statements)

References 5 publications

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“…The excellent agreement between the RIA and GLC procedures has enabled us to replace the tedious GLC assay with the much more rapid and simpleto-perform RIA for Pd-3-G. Our experience with the RIA for Pd-3-G agrees well with results obtained by groups monitoring Pd-3-G excretion in normal women. [8][9][10][11][12] Assays for~-3-G and E s-16-G were developed because oestrone and oestriol are the two major urinary oestrogens. 19 20 Although the results for E s-l6-G paralleled those for TOF, E s-16-G values were approximately one-fifth of those obtained for TOF and one-quarter of those obtained for E l-3-G ( Table 1).…”

Section: Discussionmentioning

confidence: 99%

Monitoring Induction of Ovulation by Rapid Radioimmunoassays of Oestrogen and Pregnanediol Glucuronides

et al. 1981

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SUMMARY To increase the sample-handling capacity for an induction of ovulation programme, direct urinary radioimmunoassays (RIA) for three steroid glucuronides, pregnanediol-3-glucuronide (Pd-3-G), oestrone-3-glucuronide (E c3-G), and oestriol-l 6 oc-glucuronide (E a-16-G), were established. Results obtained for urinary Pd-3-G measured by direct RIA show an excellent correlation (r =0,98, N =46) with those for urinary pregnanediol measured by gas-liquid chromatography.Estrone-3-glucuronide (E 1-3-G) and estriol-16oc-glucuronide (E a-16-G) values, measured by direct RIA, closely paralleled the total urinary oestrogen measured fluorimetrically.Ovarian response to ovulation induction therapy can be monitored by observing the changes in the levels of E 1-3-G in urine. Pre-ovulatory levels of urinary E a-16-G were found to be too low for use in this regard.Direct RIA for E 1-3-G and Pd-3-G are recommended as reliable indices of ovarian function in the monitoring of patients receiving treatment for the induction of ovulation.To assess a patient's response to ovulation induction therapy, it is usual to measure the daily output of oestrogens and pregnanediol in urine. The total urinary oestrogen levels are most commonly measured using a f1uorimetric technique.' Urinary pregnanediol levels in these non-pregnant patients are usually estimated using a ga-s-liquid chromatographic technique (GLC).2-4 Both methods are labour-intensive, requiring high levels of expertise. In view of this, alternative methods were sought which would be less demanding.In the daily monitoring of patients in an induction of ovulation programme, it is necessary to obtain urinary oestrogen assay results without delay. The results of the existing f1uorirnetric procedure are available 3 hours from receipt of the specimen in the laboratory. The aim of replacing methods for assessing ovarian response to induction of ovulation therapy was to have rapid, reliable, simple techniques with a greater specimen-handling capacity.Immunochemical methods appeared to offer the most promise as a replacement for TOF and GLC *Requests for reprints from: Mr K Outch,

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Section: Discussionmentioning

confidence: 99%

Monitoring Induction of Ovulation by Rapid Radioimmunoassays of Oestrogen and Pregnanediol Glucuronides

et al. 1981

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“…According to similar values reported in two multicenter studies conducted by the World Health Organization [16,17], and with our own standardized values (unpublished data), ovulation was defined as a rise in estrone to > 85 nmol/L, followed by another one in pregnanediol of > 10 ~tmol/L in at least two consecutive samples.…”

Section: Sample Collection and Monitoring Of Ovarian Functionmentioning

confidence: 97%

Luteinizing hormone releasing hormone agonist for postpartum contraception

et al. 1996

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GnRH agonists have advantages over steroids for hormonal contraception during the postpartum period in breastfeeding women. Symptoms of hypoestrogenism were not reported any time in either the controls or the treated groups, as estrone levels were not suppressed to menopausal values. Once-daily administration of GnRH agonist could be a reliable, acceptable and safe contraceptive method during the postpartum period in breastfeeding women. More information is required to establish GnRH analog contraceptive efficacy.

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“…On occasion, urinary analyte concentrations have been corrected by normalising to osmolality [12,13], but this is not common among reports of E1G and PdG levels. For completeness, we note that mathematical approaches to correction have also been proposed [14][15][16] and many have tried to avoid the problem by using concentration ratios of urinary analytes including E1G and PdG [17][18][19][20][21][22][23][24].…”

Section: Introductionmentioning

confidence: 99%

Expressing the quantity of urinary analytes: a discussion of some issues arising from the monitoring of the menstrual cycle

2020

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Practical domestic monitoring of the menstrual cycle requires measurements of urinary metabolites of reproductive hormones: oestrone glucuronide (E1G) and pregnanediol glucuronide (PdG). Data reported in the literature are expressed as (i) concentration, without or with either creatinine- or specific gravity correction, or (ii) excretion rates. This variation in such a fundamental issue prompts consideration of the relationships between the four measures. Because the menstrual cycle kinetics of E1G and PdG are complex, we consider measurements of urinary creatinine, urea, galactose, xylose and inulin which tend to be more stable. We show that uncorrected concentration measurements of these urinary analytes can be positively correlated, negatively correlated or uncorrelated with the serum concentration. Based on measurements of urinary creatinine concentrations, urinary specific gravity and creatinine excretion rates, we conclude that urinary analyte concentration are likely to be more reliable when creatinine-corrected rather than corrected using specific gravity, but that both are less reliable than measurements of the excretion rate. This has implications for the quantitation of any urinary analyte, but especially for the monitoring of the menstrual cycle in which changes in E1G and PdG from one day to the next can be physiologically significant for a woman monitoring her fertility.

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A possible method for the detection of ovulation and the determination of the duration of the fertile period

Cited by 26 publications

References 5 publications

Monitoring Induction of Ovulation by Rapid Radioimmunoassays of Oestrogen and Pregnanediol Glucuronides

Monitoring Induction of Ovulation by Rapid Radioimmunoassays of Oestrogen and Pregnanediol Glucuronides

Luteinizing hormone releasing hormone agonist for postpartum contraception

Expressing the quantity of urinary analytes: a discussion of some issues arising from the monitoring of the menstrual cycle

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