A portable nucleic acid detection system using natural convection combined with a smartphone

Rajendran, Vinoth Kumar; Bakthavathsalam, Padmavathy; Bergquist, Peter L.; Sunna, Anwar

doi:10.1016/j.bios.2019.03.050

Cited by 36 publications

(36 citation statements)

References 33 publications

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“…Because of our combinatory and integrated approach, the POCKET system was extremely light and small, rendering the POCKET system, to the best of our knowledge, to be one of the smallest and most portable POC DNA testing devices. Compared with other 3D-printed or microfluidic chips, our POCKET system was more versatile for a broader range of possible sample sources and achieved multiplex detections with reduced reagent costs (table S6) (6,9,11,14,17,(27)(28)(29)(30)(31).…”

Section: Discussionmentioning

confidence: 99%

An ultraportable and versatile point-of-care DNA testing platform

Xia

Wang

et al. 2020

Sci. Adv.

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Point-of-care testing (POCT) has broad applications in resource-limited settings. Here, a POCT platform termed POCKET (point-of-care kit for the entire test) is demonstrated that is ultraportable and versatile for analyzing multiple types of DNA in different fields in a sample-to-answer manner. The POCKET is less than 100 g and smaller than 25 cm in length. The kit consists of an integrated chip (i-chip) and a foldable box (f-box). The i-chip integrates the sample preparation with a previously unidentified, triple signal amplification. The f-box uses a smartphone as a heater, a signal detector, and a result readout. We detected different types of DNA from clinics to environment to food to agriculture. The detection is sensitive (<103 copies/ml), specific (single-base differentiation), speedy (<2 hours), and stable (>10 weeks shelf life). This inexpensive, ultraportable POCKET platform may become a versatile sample-to-answer platform for clinical diagnostics, food safety, agricultural protection, and environmental monitoring.

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Section: Discussionmentioning

confidence: 99%

An ultraportable and versatile point-of-care DNA testing platform

Xia

Wang

et al. 2020

Sci. Adv.

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“…Although this method still requires additional incubation and multiple washing steps, these manipulation steps can be readily streamlined on a LionX system (see details in the section Integrated Lab-in/on-an-X Systems). 57,58 Recent literature also reported different types of paper-/membrane-based extraction methods that do not require complex device fabrication and the use of laboratory equipment for the centrifugation, separation, and elution steps (Figure 2C). For example, filtration-based isolation of NAs has been developed by using cellulose paper discs (Fusion 5 filters) [59][60][61] and the commercial Whatman Flinders Technology Associates (FTA) cards.…”

Section: Progress and Potentialmentioning

confidence: 99%

“…For example, by using a smartphone fluorescence reader, a detection limit of 2.8 3 10 3 DNA copies was achieved. 57 Another type of optical readout for NA testing is the colorimetric signal, which can be observed on LFT strips. Conventionally, the LFT is used for rapid testing of protein markers.…”

Section: Optical Detectionmentioning

confidence: 99%

Fighting COVID-19: Integrated Micro- and Nanosystems for Viral Infection Diagnostics

Qin

Peng

Baravik

et al. 2020

Matter

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The pandemic of coronavirus disease 2019 (COVID-19) highlights the importance of rapid and sensitive diagnostics of viral infection that enables the efficient tracing of cases and the implementation of public health measures for disease containment. The immediate actions from both academia and industry have led to the development of many COVID-19 diagnostic systems that have secured fast-track regulatory approvals and have been serving our healthcare frontlines since the early stage of the pandemic. On diagnostic technologies, many of these clinically validated systems have significantly benefited from the recent advances in micro-and nanotechnologies in terms of platform design, analytical method, and system integration and miniaturization. The continued development of new diagnostic platforms integrating micro-and nanocomponents will address some of the shortcomings we have witnessed in the existing COVID-19 diagnostic systems. This Perspective reviews the previous and ongoing research efforts on developing integrated microand nanosystems for nucleic acid-based virus detection, and highlights promising technologies that could provide better solutions for the diagnosis of COVID-19 and other viral infectious diseases. With the summary and outlook of this rapidly evolving research field, we hope to inspire more research and development activities to better prepare our society for future public health crises.

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“…[ 42,43 ] These reported battery‐operated devices can amplify the target DNA in less than 30 min and are user‐friendly and portable. [ 44 ] PoCDs based on synthetic NA (Aptasensor), have been recently reported [ 45 ] (Figure 2B) and have been discussed in the Supporting Information.…”

Section: Biomedical Diagnosticsmentioning

confidence: 99%

Emerging Trends in Microfluidics Based Devices

Solanki

Pandey

Gupta

et al. 2020

Biotechnology Journal

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One of the major challenges for scientists and engineers today is to develop technologies for the improvement of human health in both developed and developing countries. However, the need for cost-effective, high-performance diagnostic techniques is very crucial for providing accessible, affordable, and high-quality healthcare devices. In this context, microfluidic-based devices (MFDs) offer powerful platforms for automation and integration of complex tasks onto a single chip. The distinct advantage of MFDs lies in precise control of the sample quantities and flow rate of samples and reagents that enable quantification and detection of analytes with high resolution and sensitivity. With these excellent properties, microfluidics (MFs) have been used for various applications in healthcare, along with other biological and medical areas. This review focuses on the emerging demands of MFs in different fields such as biomedical diagnostics, environmental analysis, food and agriculture research, etc., in the last three or so years. It also aims to reveal new opportunities in these areas and future prospects of commercial MFDs.

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A portable nucleic acid detection system using natural convection combined with a smartphone

Cited by 36 publications

References 33 publications

An ultraportable and versatile point-of-care DNA testing platform

An ultraportable and versatile point-of-care DNA testing platform

Fighting COVID-19: Integrated Micro- and Nanosystems for Viral Infection Diagnostics

Emerging Trends in Microfluidics Based Devices

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