Abstract:The pandemic of coronavirus disease 2019 (COVID-19) highlights the importance of rapid and sensitive diagnostics of viral infection that enables the efficient tracing of cases and the implementation of public health measures for disease containment. The immediate actions from both academia and industry have led to the development of many COVID-19 diagnostic systems that have secured fast-track regulatory approvals and have been serving our healthcare frontlines since the early stage of the pandemic. On diagnos… Show more
“…In short, they are able to perform many laborious benchtop protocols without human applications in clinical diagnostics and low-cost mass production. LOC-based techniques are widely used for viral detection in clinical applications like in the cases of Ebola, dengue fever hepatitis and Human immunodeficiency virus (HIV) as well other cases [ (26) , (27) , (28) ]. The LOC concept has been expanded using different types of configurations called LionX systems adopting miniaturized fluidic manipulation platforms and automated virus detection.…”
Section: Lab-on-a-chip (Loc) and Related Platformsmentioning
confidence: 99%
“…The LOC concept has been expanded using different types of configurations called LionX systems adopting miniaturized fluidic manipulation platforms and automated virus detection. As an example LOC-related platforms are depicted in Figure 1 and include Lab-in-a Tube, Lab-in-a Box and even Lab on-a-Drone systems among others [ 28 ]. Figure 1 The LionX Systems for NA-Based Virus Detection (Reproduced with permission from Quin et al 2020 ([ 28 ]).…”
Section: Lab-on-a-chip (Loc) and Related Platformsmentioning
confidence: 99%
“…As an example LOC-related platforms are depicted in Figure 1 and include Lab-in-a Tube, Lab-in-a Box and even Lab on-a-Drone systems among others [ 28 ]. Figure 1 The LionX Systems for NA-Based Virus Detection (Reproduced with permission from Quin et al 2020 ([ 28 ]). …”
Section: Lab-on-a-chip (Loc) and Related Platformsmentioning
confidence: 99%
“… The LionX Systems for NA-Based Virus Detection (Reproduced with permission from Quin et al 2020 ([ 28 ]). …”
Section: Lab-on-a-chip (Loc) and Related Platformsmentioning
The WHO has declared the COVID-19 epidemic on January 31, 2020. This virus has infected millions of people worldwide in just a few months. Shortly afterwards, the National Medical Products Administration (NMPA) announced nucleic acid testing as the gold standard for virus detection. Antibody testing is used as well as a supplementary test for suspected cases where nucleic acid detection was negative. In short, nucleic acid–based polymerase chain reaction (PCR) is the mainstream detection method for clinical samples as well as for the detection of SARS-CoV-2 in wastewaters. First data collected around the globe were reported in the last few months being part of the so-called Wastewater-Based Epidemiology (WBE) approach. Selection of concentration methods and primers, laboratory inter-comparison and various modalities of PCR detection of the virus in complex wastewater matrices were flagged up as main bullets that require urgent improvement. Novel approaches to enhance sensitivity, speed and automate streamlined virus detection will be discussed here as well. This list comprises devices mainly used for clinical purposes like Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR), Digital PCR, Lab-on-a-chip (LOC) and related platforms as well as Biosensors. The last part will be devoted to the identification of biomolecules to target Covid-19 outbreak based on inflammatory response biomarkers among others. To this end this opinion paper brings for discussion the issue of PCR detection and its limitations as well as new diagnostic methods in WBE.
“…In short, they are able to perform many laborious benchtop protocols without human applications in clinical diagnostics and low-cost mass production. LOC-based techniques are widely used for viral detection in clinical applications like in the cases of Ebola, dengue fever hepatitis and Human immunodeficiency virus (HIV) as well other cases [ (26) , (27) , (28) ]. The LOC concept has been expanded using different types of configurations called LionX systems adopting miniaturized fluidic manipulation platforms and automated virus detection.…”
Section: Lab-on-a-chip (Loc) and Related Platformsmentioning
confidence: 99%
“…The LOC concept has been expanded using different types of configurations called LionX systems adopting miniaturized fluidic manipulation platforms and automated virus detection. As an example LOC-related platforms are depicted in Figure 1 and include Lab-in-a Tube, Lab-in-a Box and even Lab on-a-Drone systems among others [ 28 ]. Figure 1 The LionX Systems for NA-Based Virus Detection (Reproduced with permission from Quin et al 2020 ([ 28 ]).…”
Section: Lab-on-a-chip (Loc) and Related Platformsmentioning
confidence: 99%
“…As an example LOC-related platforms are depicted in Figure 1 and include Lab-in-a Tube, Lab-in-a Box and even Lab on-a-Drone systems among others [ 28 ]. Figure 1 The LionX Systems for NA-Based Virus Detection (Reproduced with permission from Quin et al 2020 ([ 28 ]). …”
Section: Lab-on-a-chip (Loc) and Related Platformsmentioning
confidence: 99%
“… The LionX Systems for NA-Based Virus Detection (Reproduced with permission from Quin et al 2020 ([ 28 ]). …”
Section: Lab-on-a-chip (Loc) and Related Platformsmentioning
The WHO has declared the COVID-19 epidemic on January 31, 2020. This virus has infected millions of people worldwide in just a few months. Shortly afterwards, the National Medical Products Administration (NMPA) announced nucleic acid testing as the gold standard for virus detection. Antibody testing is used as well as a supplementary test for suspected cases where nucleic acid detection was negative. In short, nucleic acid–based polymerase chain reaction (PCR) is the mainstream detection method for clinical samples as well as for the detection of SARS-CoV-2 in wastewaters. First data collected around the globe were reported in the last few months being part of the so-called Wastewater-Based Epidemiology (WBE) approach. Selection of concentration methods and primers, laboratory inter-comparison and various modalities of PCR detection of the virus in complex wastewater matrices were flagged up as main bullets that require urgent improvement. Novel approaches to enhance sensitivity, speed and automate streamlined virus detection will be discussed here as well. This list comprises devices mainly used for clinical purposes like Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR), Digital PCR, Lab-on-a-chip (LOC) and related platforms as well as Biosensors. The last part will be devoted to the identification of biomolecules to target Covid-19 outbreak based on inflammatory response biomarkers among others. To this end this opinion paper brings for discussion the issue of PCR detection and its limitations as well as new diagnostic methods in WBE.
“…Also the extractions kits are less than the ramping up demand of tests [ 26 ]. During transportation, the denaturation of sample may occur which leads to false negative results [ 27 ]. In case of fully recovered patients, PCR testing can even result false positive outcome detecting genetic material of inactive SARS-CoV-2 which is actually not reinfection.…”
Section: Current Detection Approaches For
Sars-cov-2mentioning
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