A portable digital-control electrochemical system with automatic ohmic drop compensation for fast scan voltammetry and its application to ultrasensitive detection of chromium(III)

Chen, Geng; Xie, Jun; Zhang, Zhenghuan; Meng, Weiqiang; Zhang, Chunfeng; Kang, Kai; Wu, Yangbo; Guo, Zhiyong

doi:10.1016/j.snb.2019.127135

Cited by 18 publications

(8 citation statements)

References 35 publications

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“…For analysis, the instructions are sent to the circuit board by the MCU via programming. The MCU controls the programmable digital-to-analog converter (DAC) to stimulate the desired waveform from the pin of the potentiostat where the voltage applied to the sensor is modulated. , The MCU internal timer is used to count the time of applied voltage. The program stopped running when the time was up, and the LCM1602 LCD screen was used for visual effects.…”

Section: Results and Discussionmentioning

confidence: 99%

Electric Field-Induced Specific Preconcentration to Enhance DNA-Based Electrochemical Sensing of Hg²⁺ via the Synergy of Enrichment and Self-Cleaning

Liu

Chen

et al. 2022

J. Agric. Food Chem.

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Efficient preconcentration is critical for sensitive and selective electrochemical detection of metal ions, but rapid specific enrichment with depressed absorption of interfering ions at the electrode is challenging. Here, we proposed an electric fieldinduced specific preconcentration to boost the analytical performance of DNA-based electrochemical sensors for Hg 2+ detection. As for such preconcentration, a positive external electric field was first used to enrich Hg 2+ at an electrode assembled with T-rich DNA, thus boosting T−Hg 2+ −T recognitions. The following applied inverse electric field strips the nonspecifically absorbed Hg 2+ and other interfering ions, thus depressing matrix interferences via self-cleaning. Based on this principle, we designed a portable device to realize programmable control of electric fields; a T−Hg 2+ −T recognition-based electrochemical sensor was thus fabricated as a model platform to assess the feasibility of electric field-induced preconcentration. The experimental results revealed that such a strategy decreased the time of T−Hg 2+ −T-based recognition from 60 to 20 min and led to detection with better reproducibility by depressing the influence of free Hg 2+ as well as interfering ions. This strategy offered Hg 2+ detection limits of 0.01 pMthree-fold better than that without preconcentrationwithin 22 min. The proposed preconcentration strategy offers a new way to enhance the analytical performance of sensing at the solid−liquid interface.

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Section: Results and Discussionmentioning

confidence: 99%

Electric Field-Induced Specific Preconcentration to Enhance DNA-Based Electrochemical Sensing of Hg²⁺ via the Synergy of Enrichment and Self-Cleaning

Liu

Chen

et al. 2022

J. Agric. Food Chem.

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“…To realize the rapid field detection of GMOs, a portable equipment with both ECL and FSV testing capabilities is still required, which is being developed in our group. 50,51 ■ CONCLUSIONS In summary, a dual-mode electrochemical biosensor for the detection of genetically modified soybean was developed based on the CRISPR/Cas12a system, which has significant advantages in the following aspects: (1) High sensitivity the LOD was as low as 0.3 fmol/L, affording the detection of low-concentration genetically modified ingredients. (2) High selectivitycommon interferences will not affect the detection.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…To realize the rapid field detection of GMOs, a portable equipment with both ECL and FSV testing capabilities is still required, which is being developed in our group. , …”

Section: Resultsmentioning

confidence: 99%

A CRISPR/Cas12a-Mediated Dual-Mode Electrochemical Biosensor for Polymerase Chain Reaction-Free Detection of Genetically Modified Soybean

Wang

Lin

et al. 2021

Anal. Chem.

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A clustered regularly interspaced short palindromic repeats (CRISPR)/ Cas12a-mediated dual-mode electrochemical biosensor without polymerase chain reaction (PCR) amplification was designed for sensitive and reliable detection of genetically modified soybean SHZD32-1. A functionalized composite bionanomaterial Fe 3 O 4 @AuNPs/DNA-Fc&Ru was synthesized as the signal unit, while a characteristic gene fragment of SHZD32-1 was chosen as the target DNA (tDNA). When Cas12a, crRNA, and tDNA were present simultaneously, a ternary complex Cas12a-crRNA-tDNA was formed, and the nonspecific cleavage ability of the CRISPR/Cas12a system toward single-stranded DNA was activated. Thus, the single-stranded DNA-Fc in the signal unit was cleaved, resulting in the decrease in the fast scan voltammetric (FSV) signal from ferrocene (Fc) and the increase in the electrochemiluminescence (ECL) signal from ruthenium complex (Ru) inhibited by Fc. The linear range was 1−10 7 fmol/L for ECL and 10−10 8 fmol/L for FSV, and the limit of detection (LOD) was 0.3 fmol/L for ECL and 3 fmol/L for FSV. Accuracy, precision, stability, selectivity, and reliability were all satisfied. In addition, PCR-free detection could be completed in an hour at room temperature without requiring complicated operation and sample processing, showing great potential in the field detection of genetically modified crops.

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“…More details on the step by step printing and sintering processes of the gold electrodes can be found in Figure S2. It must be stressed at this point that achieving a high conductivity is essential to ensure minimal IR drop for any cell regardless of its position in the array, this increases the sensor sensitivity at higher scan rates during the electrochemical measurements [27] …”

Section: Resultsmentioning

confidence: 99%

Voltammetry in Two‐Electrode Mode for Rapid Electrochemical Screening Using a Fully Printed and Flexible Multiplexer Sensor

Nagar

Silva

2021

ChemElectroChem

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A multiplexed electrochemical platform has been designed for electrochemical screening applications, such as bacteria detection. It includes 16×16 electrochemical cell array in a two‐electrode mode fabricated by a combination of screen printing, inkjet printing, and flash‐light sintering techniques wherein each cell is addressed sequentially by a portable multiplexer potentiostat. This multiplexer unit is capable of making a total of 256 independent voltametric measurements that were validated by performing cyclic voltammetry in two‐electrode mode using four different redox mediators: [Fe(CN)6]3−/4−, FcMeOH, Ru[(NH3)6]Cl3 and K2[IrCl6]. The smart design and the use of mass production techniques makes the multiplexer device applicable for numerous electrochemical applications including health, food, and environmental monitoring.

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A portable digital-control electrochemical system with automatic ohmic drop compensation for fast scan voltammetry and its application to ultrasensitive detection of chromium(III)

Cited by 18 publications

References 35 publications

Electric Field-Induced Specific Preconcentration to Enhance DNA-Based Electrochemical Sensing of Hg²⁺ via the Synergy of Enrichment and Self-Cleaning

Electric Field-Induced Specific Preconcentration to Enhance DNA-Based Electrochemical Sensing of Hg²⁺ via the Synergy of Enrichment and Self-Cleaning

A CRISPR/Cas12a-Mediated Dual-Mode Electrochemical Biosensor for Polymerase Chain Reaction-Free Detection of Genetically Modified Soybean

Voltammetry in Two‐Electrode Mode for Rapid Electrochemical Screening Using a Fully Printed and Flexible Multiplexer Sensor

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A portable digital-control electrochemical system with automatic ohmic drop compensation for fast scan voltammetry and its application to ultrasensitive detection of chromium(III)

Cited by 18 publications

References 35 publications

Electric Field-Induced Specific Preconcentration to Enhance DNA-Based Electrochemical Sensing of Hg2+ via the Synergy of Enrichment and Self-Cleaning

Electric Field-Induced Specific Preconcentration to Enhance DNA-Based Electrochemical Sensing of Hg2+ via the Synergy of Enrichment and Self-Cleaning

A CRISPR/Cas12a-Mediated Dual-Mode Electrochemical Biosensor for Polymerase Chain Reaction-Free Detection of Genetically Modified Soybean

Voltammetry in Two‐Electrode Mode for Rapid Electrochemical Screening Using a Fully Printed and Flexible Multiplexer Sensor

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Product

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Electric Field-Induced Specific Preconcentration to Enhance DNA-Based Electrochemical Sensing of Hg²⁺ via the Synergy of Enrichment and Self-Cleaning

Electric Field-Induced Specific Preconcentration to Enhance DNA-Based Electrochemical Sensing of Hg²⁺ via the Synergy of Enrichment and Self-Cleaning