A portable chemiluminescence imaging immunoassay for simultaneous detection of different isoforms of prostate specific antigen in serum

A signal-on built in-marker electrochemical aptasensor for human prostate-specific antigen based on a hairbrush-like gold nanostructure

“…Excess DTT and thiol fragments were removed from the mixture by extraction with ethyl acetate (three times, total volume of 150 µL), and the upper layer was discarded, the next step was then immediately performed. Immobilization of aptamer was done by dropping 10 μL of 10.0 μmol dm −3 aptamer solution dissolved in Tris on the HL-Au electrode surface and kept at 4 °C for 8 h 11 , 28 , 34 . Then, the electrode was rinsed with Tris, and further treated with 1.0 mmol dm −3 6-mercapto-1-hexanol at room temperature for 30 min to obtain a well aligned aptamer monolayer 11 , 28 , 34 .…”

“…Immobilization of aptamer was done by dropping 10 μL of 10.0 μmol dm −3 aptamer solution dissolved in Tris on the HL-Au electrode surface and kept at 4 °C for 8 h 11 , 28 , 34 . Then, the electrode was rinsed with Tris, and further treated with 1.0 mmol dm −3 6-mercapto-1-hexanol at room temperature for 30 min to obtain a well aligned aptamer monolayer 11 , 28 , 34 . Then, the electrode was washed again with Tris and double distilled water, respectively, to remove non-specific adsorbed thiols.…”

“…Up to now, different methods have been reported for the detection of PSA including radioimmunoassay 7 , fluorescence detection 8 , enzyme-linked immunosorbent assay 9 , surface plasmon resonance 10 , chemiluminescent immunoassay 11 , surface-enhanced Raman scattering 12 , and electrochemical methods 13 . Some of these methods employ antibody assays which are expensive, inactive, inconvenient, time-consuming and complicated, and suffer from the disadvantages of antibodies related to the production, stability, and manipulation 14 , 15 .…”

Antioxidation Role of Different Lateral Stellate Ganglion Block in Isoproterenol-Induced Acute Myocardial Ischemia in Rats

“…This immunoassay of serum samples displayed high sensitivity. 29 Determination of the Oxidative Production and Antioxidative Enzymes…”

Label-free electrochemical aptasensing of the human prostate-specific antigen using gold nanospears

“…Up to now, a lot of methods and tools have been proposed for determination of PSA including fluorescence [30,31], surface enhanced Raman scattering (SERS) [32], surface plasmon resonance [33,34], field-effect transistors [35], capillary electrophoresis [36], white-light reflectance spectroscopy [37], mass spectrometry [38], lateral flow [39], electrochemical [40][41][42][43][44][45], chemiluminescence and electrochemiluminescence [46][47][48][49][50][51][52][53], ELISA and immunoassay [54][55][56], and a point-ofcare detection chip [57]. PSA detection in the blood by most of these methods includes routine immunoassays.…”