A Polypyrrole/Three-Enzyme Electrode for Creatinine Detection

Yamato, Hitoshi; Ohwa, Masaki; Wernet, Wolfgang

doi:10.1021/ac00113a009

Cited by 74 publications

(19 citation statements)

References 15 publications

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“…Therefore, precise monitoring of creatinine in the blood is compulsory during routine check up. Most of the existing creatinine biosensors utilize creatine amidinohydrolase (CAH) as a sensing element [3][4][5]. Typically, CAH is a homodimer enzyme, which hydrolyzes creatinine into urea and sarcosine [6].…”

Section: Introductionmentioning

confidence: 99%

Chitosan-g-polyaniline: a creatine amidinohydrolase immobilization matrix for creatine biosensor

Tiwari

Shukla²

2009

Express Polym. Lett.

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Abstract.A novel matrix composed of chitosan-graft-polyaniline (CHIT-g-PANI) was electrochemically prepared to investigate the immobilization of creatine amidinohydrolase (CAH). CAH enzyme was covalently immobilized with the CHIT-g-PANI matrix using glutaraldehyde as a linker. The resulting CAH/CHIT-g-PANI biomatrix was characterized with Fourier transform infrared spectroscopy (FTIR), atomic force microscopy (AFM), contact angle measurement and cyclic voltammetry (CV) taking CHIT-g-PANI as a reference. The influence of various parameters on CAH enzyme activity within the matrix was investigated including pH, temperature, and time. The Michaelis-Menten constant and apparent activities for the CAH enzyme were calculated to be 0.51 mM and 83.59 mg/cm 2 , respectively; indicating CHIT-g-PANI matrix has a high affinity to immobilize CAH enzyme.

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Section: Introductionmentioning

confidence: 99%

Chitosan-g-polyaniline: a creatine amidinohydrolase immobilization matrix for creatine biosensor

Tiwari

Shukla²

2009

Express Polym. Lett.

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“…Among the conducting polymer modified electrodes, PEDOT has received a significant amount of attention as an electrode materials in light emitting devices, polymer batteries, electrochromic windows, etc. [37,38]. Recently, the PEDOT-modified electrode has been used for the detection and determination of pesticides [39] and also used for the immobilization of morphine molecules [40] and ferrocyanide [41].…”

Section: Introductionmentioning

confidence: 99%

Electrocatalysis and simultaneous detection of dopamine and ascorbic acid using poly(3,4-ethylenedioxy)thiophene film modified electrodes

Vasantha

Chen

2006

Journal of Electroanalytical Chemistry

264

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“…Most of the existing creatinine biosensors utilize creatine amidinohydrolase (CAH) as a sensing element [3][4][5]. Typically, CAH is a homodimer enzyme, which hydrolyzes creatinine into urea and sarcosine [6].…”

Section: Introductionmentioning

confidence: 99%

Chitosan–SiO2–multiwall carbon nanotubes nanocomposite: A novel matrix for the immobilization of creatine amidinohydrolase

Tiwari

Dhakate

2009

International Journal of Biological Macromolecules

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A Polypyrrole/Three-Enzyme Electrode for Creatinine Detection

Cited by 74 publications

References 15 publications

Chitosan-g-polyaniline: a creatine amidinohydrolase immobilization matrix for creatine biosensor

Chitosan-g-polyaniline: a creatine amidinohydrolase immobilization matrix for creatine biosensor

Electrocatalysis and simultaneous detection of dopamine and ascorbic acid using poly(3,4-ethylenedioxy)thiophene film modified electrodes

Chitosan–SiO2–multiwall carbon nanotubes nanocomposite: A novel matrix for the immobilization of creatine amidinohydrolase

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