A polyclonal CD4+ and CD8+ lymphocytosis in a patient doubly infected with HTLV‐I and HIV‐1: A clinical and molecular analysis

Ehrlich, Garth D.; Davey, Frederick R.; Kirshner, Jeffrey J.; Sninsky, John J.; Kwok, Shirley; Slamon, Dennis J.; Kalish, R; Poiesz, Bernard J.

doi:10.1002/ajh.2830300304

Cited by 58 publications

(25 citation statements)

References 55 publications

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“…Fifteen samples were from patients infected with HTLV-I and 17 samples were from patients infected with HTLV-II. The samples from both HTLV groups had a higher target-copy number than the samples from patients infected with HIV-1, because their blood is characterized by monoclonal expansion of HTLV-infected lymphocytes (36,37). We also included 10 samples from healthy blood donors to serve as negative controls.…”

Section: Resultsmentioning

confidence: 99%

Multiplex detection of four pathogenic retroviruses using molecular beacons

Vet

Majithia

Marras

et al. 1999

Proc. Natl. Acad. Sci. U.S.A.

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307

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We describe a multiplex nucleic acid assay that identifies and determines the abundance of four different pathogenic retroviruses (HIV-1, HIV-2, and human T-lymphotrophic virus types I and II). Retroviral DNA sequences are amplified in a single, sealed tube by simultaneous PCR assays, and the resulting amplicons are detected in real time by the hybridization of four differently colored, amplicon-specific molecular beacons. The color of the fluorescence generated in the course of amplification identifies which retroviruses are present, and the number of thermal cycles required for the intensity of each color to rise significantly above background provides an accurate measure of the number of copies of each retroviral sequence that were present originally in the sample. Fewer than 10 retroviral genomes can be detected. Moreover, 10 copies of a rare retrovirus can be detected in the presence of 100,000 copies of an abundant retrovirus. Ninety-six samples can be analyzed in 3 hr on a single plate, and the use of a closed-tube format eliminates crossover contamination. Utilizing previously well characterized clinical samples, we demonstrate that each of the pathogenic retroviruses can be identified correctly and no false positives occur. This assay enables the rapid and reliable screening of donated blood and transplantable tissues.

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Section: Resultsmentioning

confidence: 99%

Multiplex detection of four pathogenic retroviruses using molecular beacons

Vet

Majithia

Marras

et al. 1999

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

307

182

View full text Add to dashboard Cite

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“…The major site of HTLV-I replication in vivo appears to be CD4+ T cells (29) although less commonly B cells (30) and some CD8+ T cells (31) can be infected. Infected macrophages have not been identified in vivo.…”

Section: Discussionmentioning

confidence: 99%

Human T-cell leukemia virus type I infection of monocytes and microglial cells in primary human cultures.

Hoffman¹,

Dhib‐Jalbut

Mikovits³

et al. 1992

Proc. Natl. Acad. Sci. U.S.A.

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The pathogenesis of progressive spastic paraparesis [HTLV-I-associated myelopathy/tropical spastic paraparesis (HAM/TSP)], a serious consequence of human T-cell leukemia virus type I (HTLV-I) infection, is unclear. T and B lymphocytes can be naturally infected by HTLV-I, but the susceptibility to HTLV-I infection of other cell types that could contribute to the pathogenesis of HAM/TSP has not been determined. We found that a human monocyte cell line (T]HP-1), primary human peripheral blood monocytes, and isolated microglial cells but not astrocytes or oligodendroglial cells derived from adult human brain were infected by HTLV-I in vitro. Infection with HTLV

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“…There was no evidence for HTLV-1/11 infection, which has been linked to lymphoproliferation in the setting of HIV infection [8] in four patients tested by ELISA and polymerase chain reaction (PCR) [9].…”

Section: Naveed M Chowhan Mohammaki H Zarrabi Changes In the Lymphomentioning

confidence: 99%

“…The patient was treated with hydroxyurea on an outpatient basis and did well until the summer of 1990. She discontinued hydroxyurea and then was seen for abdominal pain on October 8,1990. The spleen was tender and enlarged and there was a tender pelvic mass.…”

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confidence: 99%