A poliploidia artificial na obtenção de melancia sem sementes

Medina, Dixier M.; Prado, O.; Mendes, A. J. T.; Roessing, C.

doi:10.1590/s0006-87051958000100005

Cited by 7 publications

(5 citation statements)

References 4 publications

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“…Similar results were found to crosses of diploid perennial ryegrass with tetraploid hybrids, in which the seeds produced in diploid plants had seedlings with germination less than 1%, resulting in the failure of endosperm biosynthesis to trigger the embryo survival and seed germination (Ahloowalia 1975). Similar behavior was found in watermelon (Citrullus lanatus) with the production of viable seeds only to crosses between tetraploid ♀ and diploid ♂ plants, whereas the crossing of diploid♀ and tetraploid♂ plants did not lead to fertilization (Medina et al 1958).…”

Section: Seed Germination and Viabilitysupporting

confidence: 72%

“…Besides, a low triploid seedlings number was found for this crossing. The incompatibility of chromosomal sets to the crosses between plants diploid♀ × tetraploid♂ was reported in Italian ryegrass and watermelon, triggering the occurrence of nonviable seeds (Ruchel et al 2015;Medina et al 1958). In spite of Italian ryegrass had a greater ratio of cross-pollination ratio, the occurrence of 95% diploid seedlings can occur due to the small self-fertilization rate, induced in this research when the plants were placed inside growth chambers to pollination and prevent the exchange of external pollen.…”

Section: Chromosome Countingmentioning

confidence: 66%

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May the crossing between diploid and tetraploid Italian ryegrass transfer glyphosate resistance to the next generation?

et al. 2020

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Italian ryegrass (Lolium multiflorum Lam.) is a grass weed found in winter cereals, with several glyphosate-resistant diploid populations. Furthermore, the use of Italian ryegrass tetraploid genotypes creates an opportunity to increase the forage demand in replacement of diploid populations. The aim of this research was to evaluate the crossing capacity between glyphosate-resistant diploid and tetraploid Italian ryegrass to transfer glyphosate resistance to the next generation. Reciprocal crosses were performed using the susceptible tetraploid cultivar INIA Titan and resistant diploid (SVA04 biotype) plants to obtain the F1 progeny. Germination testing, survival percentage, inheritance of glyphosate-resistance, and ploidy level were evaluated in F1 progeny. Crossing of susceptible tetraploid plants ♂ and glyphosateresistant diploid ♀ affected the germination and exhibited 4.5% of triploid plants. F1 progeny from the glyphosate-resistant diploid ♂ and susceptible tetraploid ♀ did not show germination problems, but 46% of the plants died during the initial seedling stage. For this progeny, 10 and 25% of the seedlings were composed of triploid and pentaploid individuals, respectively. Crosses between susceptible tetraploid ♀ and resistant diploid ♂ did not transfer the glyphosateresistant for the plants. Susceptible tetraploid ♂ crossing with diploid resistant ♀ had a reduction of 47% the glyphosate-resistant plants compared to the homozygous glyphosateresistant progeny. Overall, tetraploid Italian ryegrass can be a safe tool to reduce and manage the increase of glyphosate-resistant diploid ryegrass in field conditions.

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Section: Seed Germination and Viabilitysupporting

confidence: 72%

Section: Chromosome Countingmentioning

confidence: 66%

May the crossing between diploid and tetraploid Italian ryegrass transfer glyphosate resistance to the next generation?

et al. 2020

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“…However, chromosome number count analysis did not confirm these results, as plants with diploid, tetraploid, and mixoploid cells were observed (Table 3). The identification of tetraploid plants (2n = 44 chromosomes) by cytogenetic analysis of the roots is not always an indication that all plant cells' genomes have been duplicated (MEDINA et al, 1958). Several plants may have only one duplicated part, so only by knowing the chromosome number, stomata size, pollen grain size, fruit shape, and seed number and size can we conclude with much certainty that the treatment has had a partial or total effect.…”

Section: Resultsmentioning

confidence: 99%

“…In Experiment 2, the experimental design was completely randomized, with eight treatments, 10 replicates, and an experimental of one plant. The watermelon seeds and seedlings of the LDRO line were subjected to 0.2% colchicine using different product application methods, adapted from Medina et al (1958) and Noh et al (2012). The treatments consisted of eight colchicine application methods (T1-T8).…”

Section: Methodsmentioning

confidence: 99%

INDUCTION OF POLYPLOIDY IN WATERMELON GENOTYPE WITH POWDERY MILDEW RESISTANCE (Podosphaera xanthii)

et al. 2022

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Triploid watermelon is highly appreciated by the most demanding markets, and due to its small size, it is ideal for consumption by small families. With the growth in areas cultivated with seedless watermelon worldwide, there is a demand for the development of tetraploid germplasm to obtain triploid hybrids with better agronomic performance. This study performed two tests to induce polyploidy in the powdery mildew–resistant line developed by Embrapa Semi-Arid, LDRO, under different colchicine concentrations and application methods. In Experiment 1, the seeds were treated with colchicine (0.0%, 0.1%, and 0.2%) for 24 h and 48 h. In Experiment 2, 0.2% colchicine was applied by different methods: (a) directly on the seed (MDS) with and without scarification, (b) on seeds with radicle emission (MER), (c) at the insertion point between the hypocotyl and the root (MIHR), (d) at the seedling apex (MAP), and (e) in the inverted hypocotyl (MHI). Chromosome count (cytogenetic analysis), number of chloroplasts per pair of guard cells, number of stomata, seedling height, and hypocotyl diameter were measured. In the LDRO line, chromosomal duplication occurred in some plant cells, but it was not possible to obtain 100% tetraploid plants. Colchicine (0.2%) for 48 h without mechanical scarification induced chromosomal duplication in watermelon. The analysis of the number of chloroplasts identified the level of ploidy early, reducing the number of plants needed to be evaluated by cytogenetics, which allowed us to more accurately identify the different levels of ploidy of the plant.

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“…Geralmente, a poliploidização é induzida para contornar a esterilidade cromossômica dos híbridos interespecíficos. Entretanto, ela também pode ser utilizada para induzir a esterilidade e eliminar as sementes, como por exemplo, em banana (VANDUREN et al, 1996), uva (PARK;WAKANA, 1999;WAKANA et al, 2000) e melancia (MEDINA et al, 1958;SOUZA;QUEIRÓZ;DIAS, 1999).…”

Section: A Poliploidia Como Ferramenta No Melhoramento Genéticounclassified

Indução de poliploidia em mandioca

Silva¹

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The induction of polyploidy in cassava The polyploidy played an important role in the improvement of many species of plants, especially in cereals including intergeneric hybrids. The process of polyploidy usually produces new and desirable phenotypes or provides support for crosses of plants in breeding programs. This research work reports the result of polyploidy induction in two cassava cultivars ('Porquinho' and 'Vassourinha') by different colchicine concentrations (0.05 %, 0.10 % and 0.15 %) in culture medium exposed for 48 or 96 hours. Regenerated plants were examined cytologically by flow cytometry and chromosome counting. Additionally, analysis of the size and frequency of stomata were performed and the results showed that can be used for screening of the treated plants. Treatment with 0.1% colchicine in the culture medium for 96 hours showed a higher number of tetraploid plants produced about the number of regenerated plants in both cultivars evaluated. No significant differences among colchicine doses and duration times treatment were found in the number of tetraploids found in each treatment. However, we observed a significant difference in tetraploid production between the cultivars analyzed, indicating that there may be a genetic response to induction of chromosome doubling with colchicine.

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A poliploidia artificial na obtenção de melancia sem sementes

Cited by 7 publications

References 4 publications

May the crossing between diploid and tetraploid Italian ryegrass transfer glyphosate resistance to the next generation?

May the crossing between diploid and tetraploid Italian ryegrass transfer glyphosate resistance to the next generation?

INDUCTION OF POLYPLOIDY IN WATERMELON GENOTYPE WITH POWDERY MILDEW RESISTANCE (Podosphaera xanthii)

Indução de poliploidia em mandioca

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