A PNIPAAm-based thermosensitive hydrogel containing SWCNTs for stem cell transplantation in myocardial repair

Li, Xia; Zhou, Jin; Liu, Zhiqiang; Chen, Jun; Lu, Shuaiyao; Sun, Haiyan; Li, Junjie; Lin, Qiuxia; Yang, Boguang; Duan, Cuimi; Xing, Malcolm; Wang, Changyong

doi:10.1016/j.biomaterials.2014.03.067

Cited by 164 publications

(138 citation statements)

References 33 publications

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“…The 3D scaffold produces a biomimetic environment for cell growth and promotes cell amplification, differentiation, and osteogenic gene expressions for bone regeneration. [61][62][63][64][65][66] When the ADSCs grow in the layer-by-layer paper-stacking 3D scaffolds, they have more osteogenic gene expressions.…”

mentioning

confidence: 99%

Layer-by-layer paper-stacking nanofibrous membranes to deliver adipose-derived stem cells for bone regeneration

Wan

Zhang

et al. 2015

IJN

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Bone tissue engineering through seeding of stem cells in three-dimensional scaffolds has greatly improved bone regeneration technology, which historically has been a constant challenge. In this study, we researched the use of adipose-derived stem cell (ADSC)-laden layer-by-layer paper-stacking polycaprolactone/gelatin electrospinning nanofibrous membranes for bone regeneration. Using this novel paper-stacking method makes oxygen distribution, nutrition, and waste transportation work more efficiently. ADSCs can also secrete multiple growth factors required for osteogenesis. After the characterization of ADSC surface markers CD29, CD90, and CD49d using flow cytometry, we seeded ADSCs on the membranes and found cells differentiated, with significant expression of the osteogenic-related proteins osteopontin, osteocalcin, and osteoprotegerin. During 4 weeks in vitro, the ADSCs cultured on the paper-stacking membranes in the osteogenic medium exhibited the highest osteogenic-related gene expressions. In vivo, the paper-stacking scaffolds were implanted into the rat calvarial defects (5 mm diameter, one defect per parietal bone) for 12 weeks. Investigating with microcomputer tomography, the ADSC-laden paper-stacking membranes showed the most significant bone reconstruction, and from a morphological perspective, this group occupied 90% of the surface area of the defect, produced the highest bone regeneration volume, and showed the highest bone mineral density of 823.06 mg/cm 3 . From hematoxylin and eosin and Masson staining, the new bone tissue was most evident in the ADSC-laden scaffold group. Using quantitative polymerase chain reaction analysis from collected tissues, we found that the ADSC-laden paper-stacking membrane group presented the highest osteogenic-related gene expressions of osteocalcin, osteopontin, osteoprotegerin, bone sialoprotein, runt-related transcription factor 2, and osterix (two to three times higher than the control group, and 1.5 times higher than the paper-stacking membrane group in all the genes). It is proposed that ADSC-laden layer-by-layer paper-stacking scaffolds could be used as a way of promoting bone defect treatment.

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mentioning

confidence: 99%

Layer-by-layer paper-stacking nanofibrous membranes to deliver adipose-derived stem cells for bone regeneration

Wan

Zhang

et al. 2015

IJN

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“…One week after treatment significant enhanced engraftment of seeding cells was detected when hydrogels were co-administered with the SCs in comparison to free ADSC administration. Moreover, LV-EF and FS, infarcted area and LV wall thickness were significantly improved in the ADSC-hydrogel group [69] providing evidence for the myocardial application of carbon nanotubes.…”

Section: Hydrogels In Cell-based Therapiesmentioning

confidence: 72%

Heart regeneration after myocardial infarction using synthetic biomaterials

Pascual-Gil

Garbayo

Díaz-Herráez

et al. 2015

Journal of Controlled Release

121

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Abstract:Myocardial infarction causes almost 7.3 million deaths each year worldwide. However, current treatments are more palliative than curative. Presently, cell and protein therapies are considered the most promising alternative treatments. Clinical trials performed until now have demonstrated that these therapies are limited by protein short half-life and by low transplanted cell survival rate, prompting the development of novel cell and protein delivery systems able to overcome such limitations. In this review we discuss the advances made in the last 10 years in the emerging field of cardiac repair using biomaterial-based delivery systems with focus on the progress made on preclinical in vivo studies. Then, we focus in cardiac tissue engineering approaches, and how the incorporation of both cells and proteins together into biomaterials has opened new horizons in the myocardial infarction treatment. Finally, the ongoing challenges and the perspectives for future work in cardiac tissue engineering will also be discussed.

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“…However, the limitations of PLGA‐based microspheres are its high burst release, poor drug loading, rapid degradation, and rapid drug release issues, as well as the small amount of time it remains in the body; these issues restrict its application, so that the hybrid PLGA delivery system is necessary. Poly ( N ‐isopropylacrylamide) (PNIPAM) is a temperature‐sensitive amphiphilic polymer that contains hydrophilic segments and hydrophobic segments 10, 11. When the temperature is lower than the critical solution temperature, the hydrogen‐bonding of hydrophilic segments controls the interaction between polymer chains and water, contributing to the polymer absorbing water and the subsequent dissolution of polymer chains and a swollen state formation; when the temperature is higher than the critical solution temperature, the hydrophobic interactions of the hydrophobic segments dominate the interaction between the polymer chains and water, and the polymer chains collapse, polymer precipitation occurs and a gel‐like state appears 10, 11, 12, 13.…”

Section: Introductionmentioning

confidence: 99%

“…Poly ( N ‐isopropylacrylamide) (PNIPAM) is a temperature‐sensitive amphiphilic polymer that contains hydrophilic segments and hydrophobic segments 10, 11. When the temperature is lower than the critical solution temperature, the hydrogen‐bonding of hydrophilic segments controls the interaction between polymer chains and water, contributing to the polymer absorbing water and the subsequent dissolution of polymer chains and a swollen state formation; when the temperature is higher than the critical solution temperature, the hydrophobic interactions of the hydrophobic segments dominate the interaction between the polymer chains and water, and the polymer chains collapse, polymer precipitation occurs and a gel‐like state appears 10, 11, 12, 13. Based on the characteristics of PNIPAM, if the PNIPAM is enwrapped on the surface of PLGA microspheres, which can increase the encapsulation efficiency of PLGA by accelerating the microsphere solidification rate with the changing temperature, it can then also extend the drug release of PLGA by slowing its degradation 14.…”

Section: Introductionmentioning

confidence: 99%

PLGA‐PNIPAM Microspheres Loaded with the Gastrointestinal Nutrient NaB Ameliorate Cardiac Dysfunction by Activating Sirt3 in Acute Myocardial Infarction

Cheng

Zeng

et al. 2016

Advanced Science

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Acute myocardial infarction (AMI) is the death of cardiomyocytes caused by a lack of energy due to ischemia. Nutrients supplied by the blood are the main source of cellular energy for cardiomyocytes. Sodium butyrate (NaB), a gastrointestinal nutrient, is a short‐chain fatty acid (butyric acid) that may act as an energy source in AMI therapy. Poly(lactic‐co‐glycolic acid)‐Poly (N‐isopropylacrylamide) microspheres loaded with NaB (PP‐N) are synthesized to prolong the release of NaB and are injected into ischemic zones in a Sprague–Dawley rat AMI model. Here, this study shows that PP‐N can significantly ameliorate cardiac dysfunction in AMI, and NaB can specially bind to Sirt3 structure, activating its deacetylation ability and inhibiting the generation of reactive oxygen species, autophagy, and angiogenesis promotion. The results indicate that NaB, acting as a nutrient, can protect cardiomyocytes in AMI. These results suggest that the gastrointestinal nutrient NaB may be a new therapy for AMI treatment, and PP‐N may be the ideal therapeutic regimen.

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A PNIPAAm-based thermosensitive hydrogel containing SWCNTs for stem cell transplantation in myocardial repair

Cited by 164 publications

References 33 publications

Layer-by-layer paper-stacking nanofibrous membranes to deliver adipose-derived stem cells for bone regeneration

Layer-by-layer paper-stacking nanofibrous membranes to deliver adipose-derived stem cells for bone regeneration

Heart regeneration after myocardial infarction using synthetic biomaterials

PLGA‐PNIPAM Microspheres Loaded with the Gastrointestinal Nutrient NaB Ameliorate Cardiac Dysfunction by Activating Sirt3 in Acute Myocardial Infarction

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A PNIPAAm-based thermosensitive hydrogel containing SWCNTs for stem cell transplantation in myocardial repair

Cited by 164 publications

References 33 publications

Layer-by-layer paper-stacking nanofibrous membranes to deliver adipose-derived stem cells&nbsp;for bone regeneration

Layer-by-layer paper-stacking nanofibrous membranes to deliver adipose-derived stem cells&nbsp;for bone regeneration

Heart regeneration after myocardial infarction using synthetic biomaterials

PLGA‐PNIPAM Microspheres Loaded with the Gastrointestinal Nutrient NaB Ameliorate Cardiac Dysfunction by Activating Sirt3 in Acute Myocardial Infarction

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Resources

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Layer-by-layer paper-stacking nanofibrous membranes to deliver adipose-derived stem cells for bone regeneration

Layer-by-layer paper-stacking nanofibrous membranes to deliver adipose-derived stem cells for bone regeneration