A pilot study of serum microRNA signatures as a novel biomarker for occult hepatitis B virus infection

Chen, Yuanyuan; Liu, Limin; Zhou, Zhenxian; Wang, Nan; Zhang, Chenyu; Zen, Ke

doi:10.1007/s00430-011-0223-0

Cited by 40 publications

(29 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A combination of the serum levels of miR-361-5p, miR-889 and miR-576-3p were shown to distinguish pulmonary tuberculosis patients from healthy individuals [26]. Li and colleagues found that four miRNAs, let-7c, miR-23b, miR-122 and miR-150, were differentially expressed in the sera of patients with occult hepatitis virus B infection (OBI) and showed a high level of accuracy in discriminating OBI cases from the non-infected controls [43]. To identify potential biomarkers for this novel H7N9 virus infection, we evaluated four serum miRNAs (miR-17, miR-20a, miR-106a and miR-376c) by ROC curve analysis.…”

Section: Discussionmentioning

confidence: 99%

Comprehensive Characterization of Serum MicroRNA Profile in Response to the Emerging Avian Influenza A (H7N9) Virus Infection in Humans

Zhu

et al. 2014

Viruses

View full text Add to dashboard Cite

A novel avian-origin influenza A (H7N9) virus recently occurred in China and caused 137 human infection cases with a 32.8% mortality rate. Although various detection procedures have been developed, the pathogenesis of this emerging virus in humans remains largely unknown. In this study, we characterized serum microRNA (miRNA) profile in response to H7N9 virus infection using TaqMan Low Density Arrays. Upon infection, a total of 395 miRNAs were expressed in the serum pool of patients, far beyond the 221 in healthy controls. Among the 187 commonly expressed miRNAs, 146 were up-regulated and only 7 down-regulated in patients. Further analysis by quantitative RT-PCR revealed that the serum levels of miR-17, miR-20a, miR-106a and miR-376c were significantly elevated in patients compared with healthy individuals (p < 0.05). Receiver operating characteristic (ROC) curves were constructed to show that each miRNA could discriminate H7N9 patients from controls with area under the curve (AUC) values ranging from 0.622 to 0.898, whereas a combination of miR-17, miR-20a, miR-106a and miR-376c obtained a higher discriminating ability with an AUC value of 0.96. Our findings unravel the significant alterations in serum miRNA expression following virus infection and manifest great potential of circulating miRNAs for the diagnosis of viral diseases.

show abstract

Section: Discussionmentioning

confidence: 99%

Comprehensive Characterization of Serum MicroRNA Profile in Response to the Emerging Avian Influenza A (H7N9) Virus Infection in Humans

Zhu

et al. 2014

Viruses

View full text Add to dashboard Cite

show abstract

“…For example, miR-150 might inhibit replication of H1N1 virus by targeting the PB2 gene, which is one component of ribonucleoprotein responsible for virus RNA replication and transcription [53]. Moreover, miR-150, let-7c, miR-23b and miR-122 were differentially expressed in occult hepatitis virus B infection (OBI) sera compared to healthy control sera, and thus, these four miRNAs have a potential to be biomarker for OBI detection [54].…”

Section: Other Virus Infectionsmentioning

confidence: 99%

MicroRNA-150: A potential regulator in pathogens infection and autoimmune diseases

Huang¹,

Zhang²,

Li³

et al. 2015

Autoimmunity

View full text Add to dashboard Cite

MicroRNAs (miRNAs) are short non-coding RNAs that play an important role in post-transcriptional regulation of gene expression. The past studies showed that miR-150 might emerge as a master regulator of gene expression during the immune cells differentiation and immune response process. Its regulation ability in immune cellular process might contribute to the host defense against invading pathogens, and dysregulated expression of miR-150 in immune cells might result in autoimmune diseases. This review summarized that miR-150 could regulate B cells, T cells and NK/iNKT cells differentiation and immune response. And also, this review provides a comprehensive view on the association of miR-150 and autoimmune diseases such as systemic sclerosis (SSc), multiple sclerosis, rheumatoid arthritis, systemic lupus erythematosus and contact sensitivity. Especially, the duplex role of miR-150 in the fibrosis process might contribute to the pathomechanism of SSc. Though much remains to be explored about the roles of miR-150 in pathogenic infection and autoimmune diseases, targeting miR-150 may serve as a promising therapy strategy.

show abstract

“…New animal models for HBV transmission have been developed, such as inoculating chimeric mice with human hepatocytes by human HBV [97]. In addition, a recent study claimed great diagnostic accuracy of the differential expression of HBV microRNA profile in serum for identifying OBI [98]. However, for the vast majority of HBV infections located in the developing countries, routine serologic screening with HBsAg and anti-HBc remains the best affordable strategy for the time being and probably for at least a decade ahead.…”

Section: Discussionmentioning

confidence: 99%

Residual Risk of Hepatitis-B-Infected Blood Donations: Estimation Methods and Perspectives

Kupek

2013

ISRN Infectious Diseases

View full text Add to dashboard Cite

Despite a considerable reduction of the risk of HBV-infected blood donation entering blood supply (residual risk) due to improved screening by HBV NAT in the developed countries, the bulk of the people with HBV living in the developing countries still needs to be screened by serologic tests such as HBsAg and anti-HBc. Many of these countries lack resources for implementing NAT and are likely to remain so in the next decade or longer, thus depending on the HBV residual risk monitoring based on serologic testing and corresponding estimation methods. This paper reviews main HBV residual risk findings worldwide and the methods based on serology used for their calculation with repeat donors, as well as their extension to the first-time donors. Two artificial datasets with high (4.36%) and low (0.48%) HBV prevalence were generated to test the performance of five methods: the original incidence/window-period model based solely on HBsAg, its modification by Soldan in 2003, the Müller-Breitkreutz model, the HBsAg yield model, and its extension to include anti-HBc seroconversions within a year. The last model was closest to the true values of residual risk and had smallest variation of the estimates in both high and low prevalence data. It may be used for residual risk evaluation in relatively small samples, such as regional blood banks data.

show abstract

A pilot study of serum microRNA signatures as a novel biomarker for occult hepatitis B virus infection

Cited by 40 publications

References 39 publications

Comprehensive Characterization of Serum MicroRNA Profile in Response to the Emerging Avian Influenza A (H7N9) Virus Infection in Humans

Comprehensive Characterization of Serum MicroRNA Profile in Response to the Emerging Avian Influenza A (H7N9) Virus Infection in Humans

MicroRNA-150: A potential regulator in pathogens infection and autoimmune diseases

Residual Risk of Hepatitis-B-Infected Blood Donations: Estimation Methods and Perspectives

Contact Info

Product

Resources

About