A pillar-based microfilter for isolation of white blood cells on elastomeric substrate

Alvankarian, Jafar; Bahadorimehr, Alireza; Majlis, Burhanuddin Yeop

doi:10.1063/1.4774068

Cited by 53 publications

(42 citation statements)

References 83 publications

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“…[8][9][10] Therefore, a fundamental understanding of the detailed fluid dynamics of blood flow and of the distribution of the wall shear stress in small vessels is essential to help detect cardiovascular diseases and to develop preventive measures and design suitable treatments. 11 Moreover, recent developments in blood sample loading and blood/tumor cell sorting devices [12][13][14] warrant the study of the viscoelastic properties of blood in microfluidic devices. a) campo@fe.up.pt and laura@campodeano.com The manipulation of real whole blood and the study of its flow dynamics in vitro are difficult because of the cost, safety, and ethics issues involved.…”

Section: Introductionmentioning

confidence: 99%

Viscoelasticity of blood and viscoelastic blood analogues for use in polydymethylsiloxane in vitro models of the circulatory system

et al. 2013

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The non-Newtonian properties of blood are of great importance since they are closely related with incident cardiovascular diseases. A good understanding of the hemodynamics through the main vessels of the human circulatory system is thus fundamental in the detection and especially in the treatment of these diseases. Very often such studies take place in vitro for convenience and better flow control and these generally require blood analogue solutions that not only adequately mimic the viscoelastic properties of blood but also minimize undesirable optical distortions arising from vessel curvature that could interfere in flow visualizations or particle image velocimetry measurements. In this work, we present the viscoelastic moduli of whole human blood obtained by means of passive microrheology experiments. These results and existing shear and extensional rheological data for whole human blood in the literature enabled us to develop solutions with rheological behavior analogous to real whole blood and with a refractive index suited for PDMS (polydymethylsiloxane) micro-and milli-channels. In addition, these blood analogues can be modified in order to obtain a larger range of refractive indices from 1.38 to 1.43 to match the refractive index of several materials other than PDMS. V C 2013 AIP Publishing LLC.

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Section: Introductionmentioning

confidence: 99%

Viscoelasticity of blood and viscoelastic blood analogues for use in polydymethylsiloxane in vitro models of the circulatory system

et al. 2013

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“…6,7 Various particle separation techniques have been developed for a fast, accurate, and label-free approach. [8][9][10][11][12] As an ideal tool for manipulating microscale objects, microfluidics have demonstrated to separate particles and cells, including pinched flow fractionation (PFF), [9][10][11][12] cross-flow filtration, [13][14][15][16][17] microfluidic disk, 18 laminar vortices, 19,20 and centrifugation/inertial focusing. [21][22][23][24][25] They possess the advantage of low-cost fabrication and massive parallelization, making high throughput sample processing possible for downstream analysis.…”

Section: Introductionmentioning

confidence: 99%

“…Electronic mail: yenwenlu@ntu.edu.tw 1932-1058/2016/10(1)/011906/13/$30.00 V C 2016 AIP Publishing LLC 10, 011906-1 leukocytes (WBCs), and rare cells from whole blood. 13,14 Due to the flow direction being vertical to the filtration direction, cross-flow filtration can reduce aggregations of clogging of the particles and cells. 26 It also can prevent excessive cell deformation or minimize the shear forces.…”

Section: Introductionmentioning

confidence: 99%

Enhancement of microfluidic particle separation using cross-flow filters with hydrodynamic focusing

Chiu

Huang

2016

Biomicrofluidics

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A microfluidic chip is proposed to separate microparticles using cross-flow filtration enhanced with hydrodynamic focusing. By exploiting a buffer flow from the side, the microparticles in the sample flow are pushed on one side of the microchannels, lining up to pass through the filters. Meanwhile a larger pressure gradient in the filters is obtained to enhance separation efficiency. Compared with the traditional cross-flow filtration, our proposed mechanism has the buffer flow to create a moving virtual boundary for the sample flow to actively push all the particles to reach the filters for separation. It further allows higher flow rates. The device only requires soft lithograph fabrication to create microchannels and a novel pressurized bonding technique to make high-aspect-ratio filtration structures. A mixture of polystyrene microparticles with 2.7 lm and 10.6 lm diameters are successfully separated. 96.2 6 2.8% of the large particle are recovered with a purity of 97.9 6 0.5%, while 97.5 6 0.4% of the small particle are depleted with a purity of 99.2 6 0.4% at a sample throughput of 10 ll/min. The experiment is also conducted to show the feasibility of this mechanism to separate biological cells with the sample solutions of spiked PC3 cells in whole blood. By virtue of its high separation efficiency, our device offers a label-free separation technique and potential integration with other components, thereby serving as a promising tool for continuous cell filtration and analysis applications. V C 2016 AIP Publishing LLC.

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“…Although magnetism and microfluidics have been recently combined; but none of them is new. Many attentions have been received for Magnetic field-based bioseparation in microfluidic devices because of its great applications in biomedical research, clinical diagnostics and biotechnological sciences [1]- [4]. In the past few years, several magnetic particle-based microfluidic bioseparation systems have been developed for separation, analysis and detection of biomolecules [5], [6].…”

Section: Introductionmentioning

confidence: 99%

The Influence of Magnetic Nanoparticles’ Size on Trapping Efficiency in a Microfluidic Device

Bahadorimehr¹,

Rashemi²,

Majlis³

2015

IJBBB

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Separation techniques in microfluidic devices using magnetic fields are well-known in biomedical applications in disease diagnosis like cancer, drug delivery, and hyperthermia. In this work, a microfluidic system is investigated. Here magnetic nanoparticles are separated from their medium using magnetic force. The whole microfluidic device for separation of functionalized magnetic beads is composed of a fluidic part and a magnetic part. The diameters of applied nanoparticles are 90 nm, 200 nm and 750 nm. In this method, magnetic nanoparticles are injected into a microchannel, the permanent magnet is located under the channel and the magnetic field which is produced by permanent magnet is applied to the nanoparticles, so the magnetic nanoparticles are integrated above the permamnet magnet. Trajectory of particles, velocity, and the location in which magnetic nanoparticles are captured are obtained.

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A pillar-based microfilter for isolation of white blood cells on elastomeric substrate

Cited by 53 publications

References 83 publications

Viscoelasticity of blood and viscoelastic blood analogues for use in polydymethylsiloxane in vitro models of the circulatory system

Viscoelasticity of blood and viscoelastic blood analogues for use in polydymethylsiloxane in vitro models of the circulatory system

Enhancement of microfluidic particle separation using cross-flow filters with hydrodynamic focusing

The Influence of Magnetic Nanoparticles’ Size on Trapping Efficiency in a Microfluidic Device

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