A physiological comparative study of acid tolerance of Lactobacillus plantarum ZDY 2013 and L. plantarum ATCC 8014 at membrane and cytoplasm levels

Guo, Yilin; Tian, Xiangge; Huang, Renhui; Tao, Xueying; Shah, Nagendra P.; Wei, Hua; Wan, Cuixiang

doi:10.1007/s13213-017-1295-x

Cited by 23 publications

(10 citation statements)

References 41 publications

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“…However, no sufficient studies on the stability of acid tolerance during continuous subculture were reported. Although we found the survival rate of the Lp30, Lp60, and Lp90 cultures in the presence of acid was lower than in the absence of acid, acid tolerance ability of L. plantarum still presented higher when compared with the results from previous research (Guo et al, 2017), and they would survive stomach acid and remain viable into the intestine. No significant difference was observed in acid tolerance among the Lp0, Lp30, Lp60, and Lp90 cultures, illustrating that acid tolerance was stable during continuous subculture.…”

Section: Discussioncontrasting

confidence: 86%

Genome-wide analysis of fermentation and probiotic trait stability in Lactobacillus plantarum during continuous culture

Feng

Zhang

Wang

et al. 2020

Journal of Dairy Science

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Trait stability of Lactobacillus plantarum was studied following daily subculture over a 90-d period. Acid and bile tolerance, self-aggregation ability, cell hydrophobicity, pathogen inhibition activity, and cholesterol removal ability of cultures subcultured 30 (Lp30), 60 (Lp60), or 90 (Lp90) times were not significantly different from the original strain (Lp0). However, carbohydrate metabolism patterns did change; the Lp0 culture was unable to use d-sorbitol, α-methyl-d-mannose, and d-raffinose, whereas Lp30, Lp60, and Lp90 cultures could. Furthermore, gluconate and gentiobiose were fully used by the Lp0 culture but only poorly used by the Lp30, Lp60, and Lp90 cultures. Milk fermentation test confirmed that L. plantarum was unable to use lactose throughout laboratory evolution. Six non-synonymous mutations in genome of the Lp30, Lp60, and Lp90 cultures were identified by whole-genome sequencing, including mutant gene encoding the phosphoglycerate mutase, which is closely related to the transport and metabolism of carbohydrates. These mutations may play an important role in changes of carbohydrate metabolism patterns observed. Understanding the evolutionary characteristics of L. plantarum will help in development for food industry.

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Section: Discussioncontrasting

confidence: 86%

Genome-wide analysis of fermentation and probiotic trait stability in Lactobacillus plantarum during continuous culture

Feng

Zhang

Wang

et al. 2020

Journal of Dairy Science

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“…This result was not limited to the presence of a polarized anode as L. plantarum also contained a significantly higher NAD + /NADH ratio when Fe 3+ was available as a terminal electron acceptor ( Figure 4—figure supplement 3 ). These NAD + /NADH ratios are more similar to those found for in E. coli performing aerobic respiration ( de Graef et al, 1999 ) or G. sulfurreducens performing anaerobic respiration than in LAB performing fermentation ( Guo et al, 2017 ). Taken together, our data indicate that EET is involved in energy conservation, and the intracellular redox balance during EET mimics a respiratory rather than a fermentative process.…”

Section: Resultssupporting

confidence: 78%

Extracellular electron transfer increases fermentation in lactic acid bacteria via a hybrid metabolism

Tejedor-Sanz

Stevens

et al. 2022

eLife

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Energy conservation in microorganisms is classically categorized into respiration and fermentation; however, recent work shows some species can use mixed or alternative bioenergetic strategies. We explored the use of extracellular electron transfer for energy conservation in diverse lactic acid bacteria (LAB), microorganisms that mainly rely on fermentative metabolism and are important in food fermentations. The LAB Lactiplantibacillus plantarum uses extracellular electron transfer to increase its NAD+/NADH ratio, generate more ATP through substrate-level phosphorylation, and accumulate biomass more rapidly. This novel, hybrid metabolism is dependent on a type-II NADH dehydrogenase (Ndh2) and conditionally requires a flavin-binding extracellular lipoprotein (PplA) under laboratory conditions. It confers increased fermentation product yield, metabolic flux, and environmental acidification in laboratory media and during kale juice fermentation. The discovery of a single pathway that simultaneously blends features of fermentation and respiration in a primarily fermentative microorganism expands our knowledge of energy conservation and provides immediate biotechnology applications.

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“…Probiotics have been found to have their cellular energy metabolism and integrity disrupted by pH drops in their immediate environment, a condition termed as acid stress, which would consequently disrupt the functionality of the probiotic. Similar trend was observed by Guo et al (2017) in an evaluative study carried out to compare the acid-stress tolerance of two L. plantarum strains: Lactobacillus plantarum ZDY 2013 and L. plantarum ATCC 8014 at cytoplasmic and membranous levels. Their findings showed that though the L. plantarum ZDY 2013 proved more tolerant to pH drop, noticeable amounts of deleterious alterations were affected by the pH change on their cellular organelles and energy metabolism.…”

Section: Growth Profile Of Probiotic Strains In Flaxseed Mediumsupporting

confidence: 81%

Optimization of flaxseed milk fermentation for the production of functional peptides and estimation of their bioactivities

Sharma

Kaur

et al. 2020

Food sci. technol. int.

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Bioactive peptides are protein fragments which have a positive impact on the functions and conditions of living organisms. Apart from other animal and plant sources flaxseed is an excellent source of bioactive peptides. In recent years, fermentation has been explored as effective way for bioactive peptides generation. Hence, the present study has been carried out to evaluate an indigenous Lactobacillus plantarum strain NCDC 374 for fermentation and peptides generation in flaxseed milk. Optimization of fermentation condition to obtain maximum functional properties (Proteolytic activity, Antioxidant activity and ACE inhibition %) was investigated using response surface methodology. Optimal condition to produce the functional peptides were found to be 4.20% inoculum size with 126 hours of fermentation time. The fermented milk resulted in 67.38% inhibition in DPPH, 41.35% inhibition in ACE and 30.38 micro gram leucine/ml proteolytic activity. Molecular weight cut off membrane (Viva spin) were used to fractionate the peptides. 10 kDa peptides showed optimal results for % DPPH inhibition, ACE inhibition, Antimicrobial activity and DPP-IV inhibition as compared to 5 kDa.

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A physiological comparative study of acid tolerance of Lactobacillus plantarum ZDY 2013 and L. plantarum ATCC 8014 at membrane and cytoplasm levels

Cited by 23 publications

References 41 publications

Genome-wide analysis of fermentation and probiotic trait stability in Lactobacillus plantarum during continuous culture

Genome-wide analysis of fermentation and probiotic trait stability in Lactobacillus plantarum during continuous culture

Extracellular electron transfer increases fermentation in lactic acid bacteria via a hybrid metabolism

Optimization of flaxseed milk fermentation for the production of functional peptides and estimation of their bioactivities

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