A phylogenetic study on galactose-containing Candida species based on 18S ribosomal DNA sequences.

Suzuki, Motofumi; Suh, Sung-Oui; Sugita, Takashi; Nakase, Takashi

doi:10.2323/jgam.45.229

Cited by 30 publications

(31 citation statements)

References 54 publications

(41 reference statements)

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“…At the time of the study, the individual test centers applying routine standard laboratory procedures performed the initial species identification, and species confirmation was performed using Fourier transform infrared spectroscopy (FT-IR). FT-IR has been reported to be a rapid, reliable, and cost-saving tool to identify and differentiate bacteria, dermatophytes, and yeasts (54)(55)(56)(57)(58)(59)(60)(61)(62). An IFS 28/B spectrophotometer (Bruker GmbH, Germany) was used for FT-IRbased identification; the resulting spectra were transformed and evaluated using Opus software from Bruker GmbH, as previously described by Tietz et al (58).…”

Section: Methodsmentioning

confidence: 99%

Phylogenetic Relationships Matter: Antifungal Susceptibility among Clinically Relevant Yeasts

Schmalreck

Lackner

Becker

et al. 2014

Antimicrob Agents Chemother

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The objective of this study was 2-fold: to evaluate whether phylogenetically closely related yeasts share common antifungal susceptibility profiles (ASPs) and whether these ASPs can be predicted from phylogeny. To address this question, 9,627 yeast strains were collected and tested for their antifungal susceptibility. Isolates were reidentified by considering recent changes in taxonomy and nomenclature. A phylogenetic (PHYLO) code based on the results of multilocus sequence analyses (large-subunit rRNA, small-subunit rRNA, translation elongation factor 1␣, RNA polymerase II subunits 1 and 2) and the classification of the cellular neutral sugar composition of coenzyme Q and 18S ribosomal DNA was created to group related yeasts into PHYLO groups. The ASPs were determined for fluconazole, itraconazole, and voriconazole in each PHYLO group. The majority (95%) of the yeast strains were Ascomycetes. After reclassification, a total of 23 genera and 54 species were identified, resulting in an increase of 64% of genera and a decrease of 5% of species compared with the initial identification. These taxa were assigned to 17 distinct PHYLO groups (Ascomycota, n ‫؍‬ 13; Basidiomycota, n ‫؍‬ 4). ASPs for azoles were similar among members of the same PHYLO group and different between the various PHYLO groups. Yeast phylogeny may be an additional tool to significantly enhance the assessment of MIC values and to predict antifungal susceptibility, thereby more rapidly initiating appropriate patient management.

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Section: Methodsmentioning

confidence: 99%

Phylogenetic Relationships Matter: Antifungal Susceptibility among Clinically Relevant Yeasts

Schmalreck

Lackner

Becker

et al. 2014

Antimicrob Agents Chemother

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“…The identification method by comparison of DNA homology has also been developed, and this method has been able to identify between crossing species. 9) Recently, the DNA sequences of the 18S ribosomal RNA gene 10) and the D1D2 domain of the 26S ribosomal RNA gene 11) (26S rDNA) sequence have been used to classify the yeast at the genus and species levels.…”

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confidence: 99%

Identification and Typing of Miso and Soy Sauce Fermentation Yeasts,Candida etchellsiiandC. versatilis, Based on Sequence Analyses of the D1D2 Domain of the 26S Ribosomal RNA Gene, and the Region of Internal Transcribed Spacer 1, 5.8S Ribosomal RNA Gene and Internal Transcribed Spacer 2

Suezawa

Kimura

Inoue

et al. 2006

Bioscience, Biotechnology, and Biochemistry

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We analyzed sequences of the D1D2 domain of the 26S ribosomal RNA gene (26S rDNA sequence), and the region of internal transcribed spacer 1, 5.8S ribosomal RNA gene and internal transcribed spacer 2 (ITS sequence) of the miso and soy sauce fermentation yeasts, Candida etchellsii and Candida versatilis, in order to evaluate the usefulness of this sequence analysis for identification and typing of these two species. In the 26S rDNA sequence method, the numbers of base substitutions among C. etchellsii strains were up to 2 in 482 bp (99.6% similarity), and they were divided into three types (types A, B, and C). Those of C. versatilis strains were also up to 2 in 521 bp (99.6% similarity) and they were divided into three types (types 1, 2, and 3). In the ITS sequence method, those of C. etchellsii strains were zero in 433 bp (type a, 100% similarity). Those of C. versatilis were 5 in 409 bp (98.8% similarity), divided into 4 types (types I, II, III and IV). It was found that molecular methods based on the sequences of the 26S rDNA D1D2 domain and the ITS region were rapid and precise compared with the physiological method for the identification and typing of these two species.

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“…The taxonomic signifi cance of cellular galactose is not clear at present. Suzuki et al (1999) reported that all galactose-containing Candida species were located in cluster II and not cluster I in the phylogenetic tree based on the small subunit rRNA gene sequences. However, these species are mingled with galactose-lacking species in the trees.…”

Section: Resultsmentioning

confidence: 99%

“…They found that, among 196 species and 9 varieties examined, 31 species and 2 varieties having Q-9 and 3 species having Q-8 had galactose in addition to mannose and glucose. Further, Suzuki et al (1999) reported that these galactose-containing Candida species were located in certain phylogenetic clades mingled with galactose-lacking species.…”

mentioning

confidence: 99%

Candida potacharoeniae sp. nov. and Candida spenceri sp. nov., two novel galactose-containing ascomycetous anamorphic yeast species isolated in Thailand

Nakase

Jindamorakot

Imanishi

et al. 2010

J. Gen. Appl. Microbiol.

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IntroductionAscomycetous yeasts usually have glucan-mannan type cell walls but species with glucan-galactomannan type cell walls are also found. Gorin and Spencer (1970) carried out extensive studies on mannosecontaining polysaccharides of yeasts, which were alkali-extracted and purifi ed via copper-complexes, by proton magnetic resonance spectra as in aid of identifi cation and chemotaxonomy. In their studies, several ascomycetous yeast species have galactose in addi-J. Gen. Appl. Microbiol., 56, 287 295 (2010) Key Words-Candida potacharoeniae sp. nov.; Candida spenceri sp. nov.; novel anamorphic yeasts; novel yeasts from Thailand Fifteen strains of anamorphic yeasts isolated from various natural substrates collected in various places in Thailand were found to represent two novel species of anamorphic yeast genus

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A phylogenetic study on galactose-containing Candida species based on 18S ribosomal DNA sequences.

Cited by 30 publications

References 54 publications

Phylogenetic Relationships Matter: Antifungal Susceptibility among Clinically Relevant Yeasts

Phylogenetic Relationships Matter: Antifungal Susceptibility among Clinically Relevant Yeasts

Identification and Typing of Miso and Soy Sauce Fermentation Yeasts,Candida etchellsiiandC. versatilis, Based on Sequence Analyses of the D1D2 Domain of the 26S Ribosomal RNA Gene, and the Region of Internal Transcribed Spacer 1, 5.8S Ribosomal RNA Gene and Internal Transcribed Spacer 2

Candida potacharoeniae sp. nov. and Candida spenceri sp. nov., two novel galactose-containing ascomycetous anamorphic yeast species isolated in Thailand

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