A phylogenetic analysis of the wild Tulipa species (Liliaceae) of Kosovo based on plastid and nuclear DNA sequence

Abstract

In Kosovo, the genus Tulipa is represented by eight taxa, most of which form a species complex surrounding Tulipa scardica. To investigate the phylogenetic relationship of these Tulipa species a Bayesian analysis was undertaken using the ITS nuclear marker and trnL-trnF, rbcL and psbA-trnH plastid markers. The resulting phylogenetic trees show that Kosovarian Tulipa species consistently group into two main clades, the subgenera Eriostemones and Tulipa. Furthermore, our analyses provide some evidence that the subspecies of Tulipa sylvestris are genetically distinguishable, however not significantly enough to support their reclassification as species. In contrast, the markers provide some novel information to reassess the species concepts of the T. scardica complex. Our data provide support for the synonymisation of Tulipa luanica and Tulipa kosovarica under the species Tulipa serbica. Resolution and sampling limitations hinder any concrete conclusion about whether Tulipa albanica and T. scardica are true species, yet our data do provide some support that these are unique taxa and therefore should continue to be treated as such until further clarification. Overall, our work shows that genetic data will be important in determining species concepts in this genus, however, even with a molecular perspective pulling apart closely related taxa can be extremely challenging.

Analysis of Research Status and Development Trend of Nanotoxicology of Liliaceae Medicinal Plants

“…The synthesized silver nanoparticles were needed to be purified using 1 KDa regenerated cellulose membrane to remove excess chemical components, such as sodium citrate and Ag+, for reserve. After filtration by ultrafiltration centrifuge tube, the concentration of Ag + in the filtrate was measured by ICP-OES to determine the dissolution degree of silver nanoparticles (the dissolved part of silver nanoparticles solution in suspension was less than 1%) [14]. The concentration of silver nanoparticles was needed to be digested by nitric acid and hydrogen After the silver nanoparticles were uniformly dispersed by ultrasonic dispersion method, the solution of 3−3.5 mL silver nanoparticles was placed in a quartz colorimetric plate.…”

Biomorphological Analysis of Tulip Varieties on Substrates in Covered Ground in Turkestan

“…Thus, the novel aspect of the current study lies in its comprehensive exploration of various Tulipa varieties, each displaying unique responses to different substrates. Hajdari et al [34], on the other hand, takes a fundamentally different approach, delving into the phylogenetics of Tulipa species in Kosovo, with a particular emphasis on understanding their relationships and taxonomic considerations. This research stands in stark contrast to the current study, which primarily focuses on the cultivation conditions and growth of Tulipa varieties.…”