A phylogenetic analysis of genus Onobrychis and its relationships within the tribe Hedysareae (Fabaceae)

Abstract

which were previously treated in tribe Galegeae, were transferred to tribe Hedysareae by . Members of Hedysareae are commonly found in dry open habitats with a continental, temperate, or Mediterranean climate, including Eurasia, North America, and the Horn of Africa (Ahangarian et al., 2007). Some taxa of the tribe are economically important as fodder legumes due to their high protein content (Hayot Carbonero et al., 2011).Molecular analyses by Wojciechowski et al. ( 2004) and Lavin et al. (2005) showed that Caragana Fabr. was the most closely related sister group to the rest of the tribe Hedysareae.Hedysareae is included in the Inverted Repeat Lacking Clade (IRLC) group sensu Wojciechowski et al. (2000Wojciechowski et al. ( , 2004 and Wojciechowski (2003Wojciechowski ( , 2005. In more recent studies, it has been suggested that Hedysareae sensu Lock ( 2005) is a sister group to the Astragalean clade, which includes genera such as Astragalus L., Oxytropis DC., and Colutea L., in addition to Chesneya Bertol. and its close relatives . According to Lavin et al. (2005) the most recent common ancestor of the Hedysareae and the Astragalean clade originated between 25.0 and 39.2 million years ago.The genus Onobrychis is divided into 2 subgenera:

Phylogenetic relationship of some Onobrychis taxa naturally grown in Turkey based on morphology and nuclear ribosomal DNA ITS Sequences

“…nrDNA ITS sequence studies in the Onobrychis taxa were performed by Ahangarian et al (2007); Safaei Chaei Kar et al (2012);Hayot Carbonero et al (2012) and Lewke Bandara et al (2013). As a result of these studies, the distinction of the Onobrychis subgenus and sections was clearly performed similarly to our nrDNA ITS sequence study.…”

“…montana, O. viciifolia, O. alba, O. supina and O. caput-galli to examine the variability between and within species. Demdoum et al (2012) Ahangarian et al (2007), Hayot Carbonero et al (2012), and Lewke Bandara et al (2013) analyzed the sequences of nuclear ribosomal DNA internal transcript spacer (nr DNA ITS) to resolve the phylogenetic relationship among different Onobrychis species. However, phylogenetic classification based on nr DNA ITS sequences and morphological data have not been reported to date with regard to Turkish Onobrychis taxa.…”

Testing the utility of matK and ITS DNA regions for discrimination of Allium species

“…In this respect, nucleotide sequences of matK, rpoC1 rpoB, trnH-PsbA rbcL, atpF-atpH, and psbK-psbI and their combinations were recently tested for barcoding plant species by the Consortium for the Barcode of Life (CBOL) Plant Working Group (Hollingsworth et al, 2009) and matK and rbcL were accepted as a 2-locus DNA barcode. The matK DNA region has been tested for discrimination of many plant species as a single barcode region or in combination with other proposed plant barcode sequences in recent studies (Lahaye et al, 2008;Burgess et al, 2011;De Mattia et al, 2011;Du et al, 2011;Gu et al 2011;Guo et al, 2011;Li et al, 2011;Pang et al, 2011;Seberg et al, 2012;Bandara et al, 2013). matK as a barcode DNA has advantages of effective species discrimination power, high quality sequence recovery, easy experimental procedures and sequence alignment, and lack of allelic polymorphisms or multiple paralogous copies compared to nuclear DNA regions (Lahaye et al, 2008;Hollingsworth et al, 2009;Burgess et al, 2011).…”

Plastid DNA variation of the endemic species Oxytropis glandulosa Turcz. (Fabaceae)

“…The cause of this difficult species delimitation may be related to recent speciation, hybridization, introgression events, and the presence of intraspecific polyploid series, as in the genus Onobrychis Mill. (Lewke Bandara et al, 2013). Statistically supported groups, formed by the species of one section or by the species from different sections and by the haplotypes of O. glandulosa, can be identified within clade II.…”