A phosphorylcholine-modified chitosan polymer as an endothelial progenitor cell supporting matrix

Tardif, Kim; Cloutier, Isabelle; Miao, Zhi-Mei; Lemieux, Carole; St-Denis, Corinne; Winnik, Françoise M.; Tanguay, Jean‐François

doi:10.1016/j.biomaterials.2011.04.002

Cited by 24 publications

(18 citation statements)

References 40 publications

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“…A central challenge in this context is the attraction, adhesion, and proliferation of endothelial progenitor cells (EPCs) or endothelial cells (ECs) to form a complete endothelium. Several strategies to address this issue have been described: immobilization of antibodies targeting markers for EPCs such as vascular endothelial growth factor receptor 2 (VEGFR2) and platelet endothelial cell adhesion molecule (PECAM-1) [14,15]; modification of the surface with peptides such as the Arg-Gly-Asp (RGD) or Cys-Ala-Gly (CAG) sequence [16,17]; immobilization of growth factors such as the vascular endothelial growth factor (VEGF) or stromal cell-derived factor-1 (SDF-1) [18,19]; immobilization of oligonucleotides and aptamers [20,21]; and surface modification with oligosaccharides and phospholipids [22,23]. However, it is necessary to develop surfaces with improved biocompatible, bioactive, targeted, and stable biofunctionalization [24].…”

Section: Introductionmentioning

confidence: 99%

Fibronectin Adsorption on Electrospun Synthetic Vascular Grafts Attracts Endothelial Progenitor Cells and Promotes Endothelialization in Dynamic In Vitro Culture

Daum

Visser

Wild

et al. 2020

Cells

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Appropriate mechanical properties and fast endothelialization of synthetic grafts are key to ensure long-term functionality of implants. We used a newly developed biostable polyurethane elastomer (TPCU) to engineer electrospun vascular scaffolds with promising mechanical properties (E-modulus: 4.8 ± 0.6 MPa, burst pressure: 3326 ± 78 mmHg), which were biofunctionalized with fibronectin (FN) and decorin (DCN). Neither uncoated nor biofunctionalized TPCU scaffolds induced major adverse immune responses except for minor signs of polymorph nuclear cell activation. The in vivo endothelial progenitor cell homing potential of the biofunctionalized scaffolds was simulated in vitro by attracting endothelial colony-forming cells (ECFCs). Although DCN coating did attract ECFCs in combination with FN (FN + DCN), DCN-coated TPCU scaffolds showed a cell-repellent effect in the absence of FN. In a tissue-engineering approach, the electrospun and biofunctionalized tubular grafts were cultured with primary-isolated vascular endothelial cells in a custom-made bioreactor under dynamic conditions with the aim to engineer an advanced therapy medicinal product. Both FN and FN + DCN functionalization supported the formation of a confluent and functional endothelial layer.

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Section: Introductionmentioning

confidence: 99%

Fibronectin Adsorption on Electrospun Synthetic Vascular Grafts Attracts Endothelial Progenitor Cells and Promotes Endothelialization in Dynamic In Vitro Culture

Daum

Visser

Wild

et al. 2020

Cells

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“…94 Phosphorylcholine-modified chitosan has also demonstrated survival and differentiation of EPCs in increased numbers compared to a fibronectin control. 95 While EPC adherence was increased, mesenchymal stem cell adherence was decreased, demonstrating that the modified chitosan matrix provides a possibly more selective surface for EPC attachment and proliferation.…”

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confidence: 99%

Strategies and Techniques to Enhance theIn SituEndothelialization of Small-Diameter Biodegradable Polymeric Vascular Grafts

Melchiorri

Hibino

Fisher

2013

Tissue Engineering Part B: Reviews

154

147

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“…1) in the coating constructs to minimise interactions between islets and the environment. The PC moiety, which is a component of plasma cell membranes, confers hydrophilicity, haemocompatibility and resistance to non-specific protein absorption, thus inhibiting the development of fibrosis, supporting endothelial cell growth [36] and also carrying anticoagulatory properties [37,38], all of which should enhance islet survival in vivo and encourage host integration. In addition, the 40% amine group content of the PC modification for chitosan increased the solubility of the polysaccharide up to about 2 mg/ml under physiological pH conditions (pH∼7.0).…”

Section: Discussionmentioning

confidence: 99%

Nano-scale encapsulation enhances allograft survival and function of islets transplanted in a mouse model of diabetes

et al. 2012

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Aims/hypothesis The success of islet transplantation as a treatment for type 1 diabetes is currently hampered by post-transplantation loss of functional islets through adverse immune and non-immune reactions. We aimed to test whether early islet loss can be limited and transplant survival improved by the application of conformal nano-coating layers to islets. Methods Our novel coating protocol used alternate layers of phosphorylcholine-derived polysaccharides (chitosan or chondroitin-4-sulphate) and alginate as coating materials, with the binding based on electrostatic complexation. The in vitro function of encapsulated mouse islets was studied by analysing islet secretory function and cell viability. The in vivo function was evaluated using syngeneic and allogeneic transplantation in the streptozotocin-induced mouse model of diabetes. Results Nano-scale encapsulated islets retained appropriate islet secretory function in vitro and were less susceptible to complement-and cytokine-induced apoptosis than nonencapsulated control islets. In in vivo experiments using a syngeneic mouse transplantation model, no deleterious responses to the coatings were observed in host animals, and the encapsulated islet grafts were effective in reversing hyperglycaemia. Allo-transplantation of the nano-coated islets resulted in preserved islet function post-implantation in five of seven mice throughout the 1 month monitoring period. Conclusions/interpretation Nano-scale encapsulation offers localised immune protection for implanted islets, and may be able to limit early allograft loss and extend survival of transplanted islets. This versatile coating scheme has the potential to be integrated with tolerance induction mechanisms, thereby achieving long-term success in islet transplantation.

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A phosphorylcholine-modified chitosan polymer as an endothelial progenitor cell supporting matrix

Cited by 24 publications

References 40 publications

Fibronectin Adsorption on Electrospun Synthetic Vascular Grafts Attracts Endothelial Progenitor Cells and Promotes Endothelialization in Dynamic In Vitro Culture

Fibronectin Adsorption on Electrospun Synthetic Vascular Grafts Attracts Endothelial Progenitor Cells and Promotes Endothelialization in Dynamic In Vitro Culture

Strategies and Techniques to Enhance theIn SituEndothelialization of Small-Diameter Biodegradable Polymeric Vascular Grafts

Nano-scale encapsulation enhances allograft survival and function of islets transplanted in a mouse model of diabetes

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