A pH-sensitive guar gum-<i>grafted</i>-lysine-β-cyclodextrin drug carrier for the controlled release of 5-flourouracil into cancer cells

Praphakar, Rajendran Amarnath; Jeyaraj, Murugan; Mehnath, Sivaraj; Higuchi, Akon; Ponnamma, Deepalekshmi; Sadasivuni, Kishor Kumar; Rajan, Mariappan

doi:10.1039/c7tb02551c

Cited by 71 publications

(34 citation statements)

References 32 publications

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“…The surface morphology and size of the particles synthesized via DES grafted manner is shown in Figure DES have shown in agglomerate shapes; and this shape is better in terms of stability because it consists of molecules related to the homogeneity in the image, a hypothesis supported by Figure A . Based on the SEM observation of DES grafted PGA image was shown in Figure B, the DES grafted PGA is completely changed shape from agglomerate to spherical.…”

Section: Resultsmentioning

confidence: 79%

Folic Acid Conjugated Polyglutamic Acid Drug Vehicle Synthesis through Deep Eutectic Solvent for Targeted Release of Paclitaxel

et al. 2019

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Deep eutectic solvents (DESs) are promising biocompatible solvents that have many applications in biomedical research including drug delivery. In this study, nanomicelles were designed and developed for encapsulation of paclitaxel (PTX) for cancer therapy using DES from proline (PA) and lactic acid (LA) as resources. The self-assembled DES grafted polyglutamic acid conjugated folic acid (DES-g-PGA-FA) micelles were determined through Critical micelle concentration (CMC) by fluorescence analysis using pyrene as a probe (CMC = 0.7 mg/ mL). The successful preparation of DESs and DESs grafted polymer was confirmed by spectroscopy techniques, and density. The crystalline nature, thermal stability, particle size, surface morphology of the micelles and drug loaded micelles were determined by X-ray powder diffraction (XRD), Thermogravimetric analysis (TGA), and also microscope techniques analysis. In addition, the encapsulation efficiency and sustained release behavior of PTX loaded micelles was tested by UVvisible spectroscopy at the λ max value of 280 nm. Anticancer activity of the compound (PTX loaded polymeric micelles) was assessed on the human cervical cancer cell line (HeLa) and toxicity effect against normal human peripheral blood mononuclear cells (PBMC) using the tetrazolium-based MTT assay. These results demonstrate that the polymeric micelles are a promising carrier for anticancer therapeutics and minimize adverse side effects to normal cells.[a] P. Pradeepkumar, Dr. M. Rajan

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Section: Resultsmentioning

confidence: 79%

Folic Acid Conjugated Polyglutamic Acid Drug Vehicle Synthesis through Deep Eutectic Solvent for Targeted Release of Paclitaxel

et al. 2019

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“…The difference of the mass during this process was measured. A mass loss in the curves indicated that a degradation (or) phase change of the measured substance takes place . For pure theophylline, there was only one weight loss stage from 211‐294 °C, corresponding to its decomposition and pyrolysis process.…”

Section: Resultsmentioning

confidence: 99%

Controlled stimulation‐burst targeted release by pH‐sensitive HPMCAS/theophylline composite nanofibers fabricated through electrospinning

Liu

Wang

et al. 2019

J of Applied Polymer Sci

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HPMCAS/theophylline composite nanofibers, which displayed a significant pH-responsive behavior, were successfully fabricated through electrospinning. SEM observations demonstrated that the nanofibers were linear with a smooth and unordered structure. Mean diameter of the composite nanofibers increased with the content of drug. FT-IR spectra confirmed that theophylline was successfully loading into HPMCAS nanofibers. XRD, DSC, and TGA verified that theophylline blended with HPMCAS in a physical level and had retained its crystalline state in the composite nanofibers, which further demonstrated that the drug efficacy did not destroy in the electrospinning process. Drug loading testing showed that drug loading percent and encapsulation efficiency increased with the content of drug, except the 4% drug content sample, which was confirmed to be decided by the beaded morphology. Furthermore, the in vitro drug release studies revealed that the composite nanofibers particularly showed rapid theophylline release at weak alkaline pH environment and exhibited a burst release in the first hour followed by a decreased tendency. However, the release ratio was still at a high level after 12 h. The release process can be helpful to the urgent sudden disease, meanwhile to reduce the suffering of patients and the required frequency of drug administration.

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“…In one word, there is increasingly scientific evidences revealing the evil part of chemotherapy drugs in tumor treatment, including promotions of metastasis, proliferation, immune escape, and so on [1][2][3]. Therefore, to develop new treatment strategy including nanotechnology-based methodologies [4][5][6][7][8][9] for cancer has been under continuous investigation during the past a few years. One of the most highlighted progresses is the successful achievement of complete remission in clinical of B cell lymphoma after treated with chimeric antigen receptor (CAR) T cells [10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Vγ9Vδ2 T Cells Strengthen Cisplatin Inhibition Activity Against Breast Cancer MDA-MB-231 Cells by Destructing Mitochondrial Function and Cell Ultrastructure

Huang

Wang

2020

Preprint

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Background: Breast cancer ranks second of new cases and fifth of death in 2018 world widely. Chemotherapy, one of cancer therapeutic strategies, plays important role in controlling mortality of breast cancer. Cis-platinum (CDDP), one of traditional chemotherapy drugs, had been used clinically for years. The crucial limitation of CDDP is due to its adverse effects on immune system. Development of new protocol that can minimize side effect and meanwhile elevate clinical efficacy of traditional drug like CDDP will eventually benefit cancer patients. Vγ9Vδ2 T cells had been reported to be able to up-regulate immune function of cancer patients, therefore, our hypothesis is that introduction of Vγ9Vδ2 T cells could potentiate CDDP efficacy against breast cancer. Methods: In the present work, breast cancer cell line MDA-MB-231 was used a model cell to test our hypothesis. The therapeutic dose of CDDP in vitro was analyzed by flow cytometry; The cytoskeleton was visualized by using a confocal microscopy, and the ultrastructure of the membrane was observed by atomic force microscopy to observe the effect of combined action on MDA-MB-231 cells; The mitochondrial function of MDA-MB-231 cells was detected, and the relevant mechanism of Vγ9Vδ2 T in enhancing cisplatin cells' inhibition of MDA-MB-231 cells was discussed. Results: Vγ9Vδ2 T cells could enhance CDDP-induced MDA-MB-231 cell membrane ultrastructure disorder and cytoskeleton disorder, and enhance the inhibition of CDDP on MDA-MB-231 cells, of which Vγ9Vδ2 T cells enhancing CDDP-induced mitochondrial dysfunction was one of its mechanisms. Conclusion: In this study，the mechanism of Vγ9Vδ2 T cells in enhancing the inhibition of cisplatin on MDA-MB-231 cells was studied, which could provide an important scientific clue for developing effective treatment schemes for breast cancer, especially the refractory TNBC breast cancer, based on Vγ9Vδ2 T cells in the future.

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A pH-sensitive guar gum-grafted-lysine-β-cyclodextrin drug carrier for the controlled release of 5-flourouracil into cancer cells

Abstract: The physiological environment is a crucial factor in biomedical systems, which can be regulated with relative ease both in vitro and in vivo.

Cited by 71 publications

References 32 publications

Folic Acid Conjugated Polyglutamic Acid Drug Vehicle Synthesis through Deep Eutectic Solvent for Targeted Release of Paclitaxel

Folic Acid Conjugated Polyglutamic Acid Drug Vehicle Synthesis through Deep Eutectic Solvent for Targeted Release of Paclitaxel

Controlled stimulation‐burst targeted release by pH‐sensitive HPMCAS/theophylline composite nanofibers fabricated through electrospinning

Vγ9Vδ2 T Cells Strengthen Cisplatin Inhibition Activity Against Breast Cancer MDA-MB-231 Cells by Destructing Mitochondrial Function and Cell Ultrastructure

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