A perspective view on the nanomotion detection of living organisms and its features

Venturelli, Leonardo; Kohler, Anne-Céline; Stupar, Petar; Villalba, María Inés; Kalauzi, Aleksandar; Radotić, Ksenija; Bertacchi, Massimiliano; Dinarelli, Simone; Girasole, M.; Pešić, Milica; Banković, Jasna; Vela, M. E.; Yantorno, Osvaldo; Willaert, Ronnie; Dietler, Giovanni; Longo, Giovanni; Kasas, Sandor

doi:10.1002/jmr.2849

Cited by 25 publications

(17 citation statements)

References 76 publications

(99 reference statements)

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“…Our previous studies with bacteria and cells suggest that these movements represent the viability of cells and convey information about their metabolism, cytoskeleton rearrangements, etc. [ 22 , 23 ].…”

Section: Resultsmentioning

confidence: 99%

Nano-Motion Analysis for Rapid and Label Free Assessing of Cancer Cell Sensitivity to Chemotherapeutics

et al. 2021

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Background and Objectives: Optimization of chemotherapy is crucial for cancer patients. Timely and costly efficient treatments are emerging due to the increasing incidence of cancer worldwide. Here, we present a methodology of nano-motion analysis that could be developed to serve as a screening tool able to determine the best chemotherapy option for a particular patient within hours. Materials and Methods: Three different human cancer cell lines and their multidrug resistant (MDR) counterparts were analyzed with an atomic force microscope (AFM) using tipless cantilevers to adhere the cells and monitor their nano-motions. Results: The cells exposed to doxorubicin (DOX) differentially responded due to their sensitivity to this chemotherapeutic. The death of sensitive cells corresponding to the drop in signal variance occurred in less than 2 h after DOX application, while MDR cells continued to move, even showing an increase in signal variance. Conclusions: Nano-motion sensing can be developed as a screening tool that will allow simple, inexpensive and quick testing of different chemotherapeutics for each cancer patient. Further investigations on patient-derived tumor cells should confirm the method’s applicability.

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Section: Resultsmentioning

confidence: 99%

Nano-Motion Analysis for Rapid and Label Free Assessing of Cancer Cell Sensitivity to Chemotherapeutics

et al. 2021

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“…Homemade software written in LabVIEW (National Instruments, Austin, TX, USA) [ 18 ] was used to adjust data acquisition parameters. Typically, the deflection signal was collected at a sampling frequency of 20 kHz for a standard recording duration of 30 min.…”

Section: Methodsmentioning

confidence: 99%

Nanomotion Spectroscopy as a New Approach to Characterize Bacterial Virulence

et al. 2021

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Atomic force microscopy (AFM)-based nanomotion detection is a label-free technique that has been used to monitor the response of microorganisms to antibiotics in a time frame of minutes. The method consists of attaching living organisms onto an AFM cantilever and in monitoring its nanometric scale oscillations as a function of different physical-chemical stimuli. Up to now, we only used the cantilever oscillations variance signal to assess the viability of the attached organisms. In this contribution, we demonstrate that a more precise analysis of the motion pattern of the cantilever can unveil relevant medical information about bacterial phenotype. We used B. pertussis as the model organism, it is a slowly growing Gram-negative bacteria which is the agent of whooping cough. It was previously demonstrated that B. pertussis can expresses different phenotypes as a function of the physical-chemical properties of the environment. In this contribution, we highlight that B. pertussis generates a cantilever movement pattern that depends on its phenotype. More precisely, we noticed that nanometric scale oscillations of B. pertussis can be correlated with the virulence state of the bacteria. The results indicate a correlation between metabolic/virulent bacterial states and bacterial nanomotion pattern and paves the way to novel rapid and label-free pathogenic microorganism detection assays.

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“…The technique is relatively simple to set up. A detailed procedure describing the preparation measurement and the data processing steps can be found in Venturelli et al [150]. Briefly, the first step consists of functionalizing a relatively soft (0.06 N/m) AFM cantilever with a cross linking molecule such as glutaraldehyde, paraformaldehyde, APTES ((3-aminopropyl)triethoxysilane) or fibronectin.…”

Section: Afm Nanomotion Setup and Measurementmentioning

confidence: 99%

Nanomotion Detection-Based Rapid Antibiotic Susceptibility Testing

et al. 2021

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Rapid antibiotic susceptibility testing (AST) could play a major role in fighting multidrug-resistant bacteria. Recently, it was discovered that all living organisms oscillate in the range of nanometers and that these oscillations, referred to as nanomotion, stop as soon the organism dies. This finding led to the development of rapid AST techniques based on the monitoring of these oscillations upon exposure to antibiotics. In this review, we explain the working principle of this novel technique, compare the method with current ASTs, explore its application and give some advice about its implementation. As an illustrative example, we present the application of the technique to the slowly growing and pathogenic Bordetella pertussis bacteria.

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A perspective view on the nanomotion detection of living organisms and its features

Cited by 25 publications

References 76 publications

Nano-Motion Analysis for Rapid and Label Free Assessing of Cancer Cell Sensitivity to Chemotherapeutics

Nano-Motion Analysis for Rapid and Label Free Assessing of Cancer Cell Sensitivity to Chemotherapeutics

Nanomotion Spectroscopy as a New Approach to Characterize Bacterial Virulence

Nanomotion Detection-Based Rapid Antibiotic Susceptibility Testing

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