A permeabilized cell model for studying cytokinesis using mammalian tissue culture cells

Cande, W. Zacheus

doi:10.1083/jcb.87.2.326

Cited by 78 publications

(45 citation statements)

References 31 publications

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“…The pigment granules continue to move normally after NEM-HMM injection, presumably providing ample opportunity for the injected actomyosin inhibitor to locate its binding site on actin. These probes for actin and actomyosin have been shown to inhibit a diverse set of motility events including cytokinesis (7,22), some forms of axoplasmic transport (11,14), and cytoplasmic streaming (25,39,43). It seems improbable to us that none of them would be successful in affecting pigment transport if it were actin-or actomyosin-based.…”

Section: The Role Of Actin or Actomyosin In Pigment Granule Motilitymentioning

confidence: 99%

Analysis of the role of microtubules and actin in erythrophore intracellular motility.

Beckerle¹,

Porter²

1983

The Journal of Cell Biology

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The Holocentrus erythrophore, a red pigment cell, represents a model system for the study of organized intracellular transport. We have investigated the possibility that microtubules and actin are integral components of the pigment translocating motility machine. By creating cells that have total or partial loss of the microtubule framework we have demonstrated that the presence of microtubules is essential for organized, radial transport of the pigment granules. However, in the absence of microtubules, some undirected movement of the pigment can be stimulated; this suggests that a nonmicrotubular component of the cytoplast is responsible, at least in part, for the generation of motive force. In order to test the hypothesis that this component consists of actin or actomyosin, we examined the effects of probes for these classical motility proteins. Neither microinjection of phalloidin, DNase I or Nethylmaleimide-modified heavy meromyosin nor exogenous application of cytochalasin B has any effect on pigment motion, although these materials do block the actin-mediated motility of other systems in our hands. Therefore, intracellular particle transport in erythrophores does not appear to be actin or actomyosin-based.

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Section: The Role Of Actin or Actomyosin In Pigment Granule Motilitymentioning

confidence: 99%

Analysis of the role of microtubules and actin in erythrophore intracellular motility.

Beckerle¹,

Porter²

1983

The Journal of Cell Biology

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“…Under these conditions the endothelial cytoskeleton can be directly stimulated or blocked by otherwise impermeable hydrophilic molecules such as ATP, ATP analogues, or the S,-fragment of myosin cross-bridges covalently modified by N-ethylmaleimide (NEM). NEM-modified myosin-S, still binds to actin filaments but no longer dissociates from actin in the presence of ATP (Cande, 1980(Cande, , 1986. This property of myosin-S1-NEM has been used as a powerful tool in cell biology to block myosin binding sites along actin filaments and thus to selectively inhibit actomyosin-mediated cellular activities (Cande, 1980(Cande, , 1986.…”

mentioning

confidence: 99%

“…NEM-modified myosin-S, still binds to actin filaments but no longer dissociates from actin in the presence of ATP (Cande, 1980(Cande, , 1986. This property of myosin-S1-NEM has been used as a powerful tool in cell biology to block myosin binding sites along actin filaments and thus to selectively inhibit actomyosin-mediated cellular activities (Cande, 1980(Cande, , 1986.…”

mentioning

confidence: 99%

Role of actin and myosin in the control of paracellular permeability in pig, rat and human vascular endothelium.

Schnittler

Wilke

Gress

et al. 1990

The Journal of Physiology

198

128

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SUMMARY1. We have investigated the endothelial actomyosin system with particular emphasis on its possible role in actively opening a paracellular route for permeability.2. Actin and myosin comprised 16% of total endothelial protein with a molar actin/myosin ratio of 16-2 which is close to the actin/myosin ratio of muscle (studies on freshly isolated pig pulmonary arterial endothelial cells, PAEC).3. By immunocytochemistry at the light and electron microscope levels the bulk of actin and myosin was colocalized in close vicinity to the intercellular clefts of both micro-and macrovascular endothelial cells in situ and in vitro.4. Calcium-ionophore-induced rise in permeability of human umbilical venous endothelial cells (HUVEC) and PAEC monolayers grown on filters in a two-chamber permeability system was caused by opening of intercellular gaps. Gap formation depended on the rise in intracellular Ca2+ and could be blocked by the calmodulinbinding drugs trifluperazine (TFP) and W7.5. In skinned monolayers of cultured PAEC and in isolated sheets of HUVEC gap formation was shown to require ATP and occurred only when free myosin binding sites were available on endothelial actin filaments (experiments with myosin subfragment 1 modified by N-ethylmaleimide, S1-NEM).6. These experiments suggest that actin and myosin in endothelial cells play a central role in regulating the width of the intercellular clefts, thereby controlling the paracellular pathway of vascular permeability.

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“…Contractile ring constriction has been shown in both permeabilized vertebrate cells (HoffmanBerling 1954;Cande 1980) and isolated contractile rings (Mabuchi et al 1988). Actin-filament-stabilizing drugs inhibit ring constriction; thus, constriction depends on actin filament disassembly.…”

Section: Reconstitution Of the Contractile Apparatusmentioning

confidence: 99%

Cytokinesis in Metazoa and Fungi

Glotzer

2016

Cold Spring Harb Perspect Biol

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SUMMARYCell division-cytokinesis-involves large-scale rearrangements of the entire cell. Primarily driven by cytoskeletal proteins, cytokinesis also depends on topological rearrangements of the plasma membrane, which are coordinated with nuclear division in both space and time. Despite the fundamental nature of the process, different types of eukaryotic cells show variations in both the structural mechanisms of cytokinesis and the regulatory controls. In animal cells and fungi, a contractile actomyosin-based structure plays a central, albeit flexible, role. Here, the underlying molecular mechanisms are summarized and integrated and common themes are highlighted.

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A permeabilized cell model for studying cytokinesis using mammalian tissue culture cells

Cited by 78 publications

References 31 publications

Analysis of the role of microtubules and actin in erythrophore intracellular motility.

Analysis of the role of microtubules and actin in erythrophore intracellular motility.

Role of actin and myosin in the control of paracellular permeability in pig, rat and human vascular endothelium.

Cytokinesis in Metazoa and Fungi

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