Abstract:Background & objectives:Insulin regulated aminopeptidase (IRAP) has been related to certain pathologies such as breast cancer, Alzheimer's disease and septic shock. IRAP is encoded by the leucyl/cystinyl aminopeptidase (LNPEP) gene. The genetic variation in the LNPEP gene has been analyzed in relation with the mortality and vasopressin clearance in septic shock. The LNPEP rs4869317 SNP (single nucleotide polymorphism) was the most significantly associated SNP with vasopressinase activity, being TT genotype ass… Show more
“…LNPEP is an angiotensin IV receptor, which is an important component of the renin-angiotensin system (RAAS) [ 12 ]. RAAS regulates blood pressure, electrolyte, and fluid homeostasis [ 13 ].…”
Aim. Psoriasis is a chronic inflammatory disease with a complex etiology, and psoriasis vulgaris (PsV) is the most common type of psoriasis. Recent studies suggest the relationship between psoriasis and metabolic syndrome in different ethnicities. This study is aimed at evaluating the association of metabolism-related gene variants with the risk of PsV in Chinese Han population. Material and Methods. PsV patients (1030) and healthy controls (965) were enrolled in this study. Eighteen single-nucleotide polymorphisms (SNPs) previously reported to be significantly associated with metabolic syndrome were selected. SNPs were detected by next-generation sequencing. Results. Seven SNPs were significantly associated with PsV: rs805303 (
P
=
0.012
,
OR
=
0.85
), rs3177928 (
P
=
1.37
×
10
−
15
,
OR
=
2.51
), and rs2247056 (
P
=
3.73
×
10
−
4
,
OR
=
0.67
) located in the HLA gene region; rs1047781 (
P
=
0.012
,
OR
=
1.18
), rs281379 (
P
=
0.014
,
OR
=
1.71
), and rs492602 (
P
=
0.005
,
OR
=
1.86
) located in the FUT2 region; and rs2303138 (
P
=
0.014
,
OR
=
1.18
) located in the LNPEP region. After stratified analysis, rs805303 (
P
=
0.017
,
OR
=
0.74
) and rs2303138 (
P
=
0.041
,
OR
=
1.30
) were associated with PsVs when HLA-C
∗
06 : 02 was positive, and rs805303 (
P
=
5.62
×
10
−
5
,
OR
=
0.68
), rs3177928 (
P
=
0.003
,
OR
=
1.75
), rs281379 (
P
=
0.034
,
OR
=
1.96
), and rs492602 (
P
=
0.025
,
OR
=
2.04
) were associated with PsVs when HLA-C
∗
06 : 02 was negative. Conclusion. PsV and metabolic syndrome may have overlapped susceptible genes in Chinese Han population.
“…LNPEP is an angiotensin IV receptor, which is an important component of the renin-angiotensin system (RAAS) [ 12 ]. RAAS regulates blood pressure, electrolyte, and fluid homeostasis [ 13 ].…”
Aim. Psoriasis is a chronic inflammatory disease with a complex etiology, and psoriasis vulgaris (PsV) is the most common type of psoriasis. Recent studies suggest the relationship between psoriasis and metabolic syndrome in different ethnicities. This study is aimed at evaluating the association of metabolism-related gene variants with the risk of PsV in Chinese Han population. Material and Methods. PsV patients (1030) and healthy controls (965) were enrolled in this study. Eighteen single-nucleotide polymorphisms (SNPs) previously reported to be significantly associated with metabolic syndrome were selected. SNPs were detected by next-generation sequencing. Results. Seven SNPs were significantly associated with PsV: rs805303 (
P
=
0.012
,
OR
=
0.85
), rs3177928 (
P
=
1.37
×
10
−
15
,
OR
=
2.51
), and rs2247056 (
P
=
3.73
×
10
−
4
,
OR
=
0.67
) located in the HLA gene region; rs1047781 (
P
=
0.012
,
OR
=
1.18
), rs281379 (
P
=
0.014
,
OR
=
1.71
), and rs492602 (
P
=
0.005
,
OR
=
1.86
) located in the FUT2 region; and rs2303138 (
P
=
0.014
,
OR
=
1.18
) located in the LNPEP region. After stratified analysis, rs805303 (
P
=
0.017
,
OR
=
0.74
) and rs2303138 (
P
=
0.041
,
OR
=
1.30
) were associated with PsVs when HLA-C
∗
06 : 02 was positive, and rs805303 (
P
=
5.62
×
10
−
5
,
OR
=
0.68
), rs3177928 (
P
=
0.003
,
OR
=
1.75
), rs281379 (
P
=
0.034
,
OR
=
1.96
), and rs492602 (
P
=
0.025
,
OR
=
2.04
) were associated with PsVs when HLA-C
∗
06 : 02 was negative. Conclusion. PsV and metabolic syndrome may have overlapped susceptible genes in Chinese Han population.
“…However, the tremendous potential of gene detection in clinical practice has been limited thus far by multiple time-consuming steps and the need for professionally trained staff and costly equipment to obtain genotyping results. Furthermore, the results are usually analyzed by agarose gel electrophoresis (most PCR-based methods) ( Ramire-Zexpósito et al., 2016 , Wu et al., 2015 ), a further reaction step, and professional software (e.g., biochip, sequencing, and mass array) ( Nijveen et al., 2013 , Xu et al., 2012 , Yi et al., 2014 ). To address these issues, we used a magnetic lateral flow assay (MLFA) system to interpret the results through visualization or a magnetic signal reader.…”
SummaryDeveloping a sensitive, low-cost, and easy-to-use point-of-care testing system for genotyping is important for informing treatment decisions and predicting the risk of underlying diseases. Conventional methods normally require complex operational procedures as well as expensive and sophisticated instruments. Here, we report a general approach that enables us to detect the genotype of multiple sample types directly without DNA purification. Moreover, the PCR results can be further quantitatively analyzed based on a magnetic lateral flow assay (MLFA) system, which avoids multiple steps needed for conventional nucleic acid biosensors. As a demonstration, we show that three genotypes of aldehyde dehydrogenase 2 (ALDH2) can be identified using a small volume of sample with an accuracy of 100% and a sensitivity of 1.0 × 102 cells/μL, which are better than those of the gold standard methods. We believe that the direct PCR-MLFA system represents a significant advance toward the development of portable, sensitive biomedical platforms.
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