Rapid tests for pathogen identification
and spread assessment are
critical for infectious disease control and prevention. The control
of viral outbreaks requires a nucleic acid diagnostic test that is
sensitive and simple and delivers fast and reliable results. Here,
we report a one-pot direct reverse transcript loop-mediated isothermal
amplification (RT-LAMP) assay of SARS-CoV-2 based on a lateral flow
assay in clinical samples. The entire contiguous sample-to-answer
workflow takes less than 40 min from a clinical swab sample to a diagnostic
result without professional instruments and technicians. The assay
achieved an accuracy of 100% in 12 synthetic and 12 clinical samples
compared to the data from PCR-based assays. We anticipate that our
method will provide a universal platform for rapid and point-of-care
detection of emerging infectious diseases.
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