A PCR-based diagnostic tool for distinguishing grape skin color mutants

Giannetto, Sabrina; Velasco, Riccardo; Troggio, Michela; Malacarne, G.; Storchi, Paolo; Cancellier, S.; Nardi, Barbara De; Crespan, Manna

doi:10.1016/j.plantsci.2008.05.010

Cited by 20 publications

(20 citation statements)

References 23 publications

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“…Overall, the grapevine somatic variants for berry skin color can be divided in two main groups: A) unpigmented and less-pigmented variants from a pigmented ancestor, namely white and grey/pink-skinned variants derived from a black-skinned ancestor; B) pigmented variants from an unpigmented wild-type, namely red/pink-skinned variants derived from a white-skinned wild-type [11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of berry skin color reversion on grape somatic variants

Ferreira

Castro

Carrasco

et al. 2018

JBR

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BACKGROUND: During grapevine domestication somatic variation has been used as a source of diversity for clonal selection. OBJECTIVE: This work provides additional information on the molecular mechanisms responsible for berry skin color reversion on a subset of somatic variants for berry skin color never investigated before. METHODS: The berry color locus and its surrounding genomic region were genetically characterized through a layer-specific approach, which has already been proven to be a successful method to decipher the molecular mechanisms responsible for color reversions on somatic variants. RESULTS: Deletions of different extent and positions were detected among less-pigmented/unpigmented variants derived from a pigmented wild-type. These deletions affected only the inner cell layer in the less pigmented variants and both cell layers in the unpigmented variants. Regarding the pigmented variants derived from an unpigmented wild-type, only one group was distinguished by the Gret1 retrotransposon partial excision from the VvMybA1 promoter. Moreover, within this latter group, VvMybA2 showed an important role regarding the phenotypic variation, through the recovery of the functional G allele. CONCLUSIONS: This investigation focused on the berry color locus using somatic variants for berry skin color, promotes a better understanding of the evolutionary events behind their origin and variability, opening an opportunity for their use in the genetic improvement of varieties.

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Section: Introductionmentioning

confidence: 99%

Molecular characterization of berry skin color reversion on grape somatic variants

Ferreira

Castro

Carrasco

et al. 2018

JBR

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“…Cultivars with highly similar genetic background but contrasting color patterns are useful to explore the genetic basis of the color patterns by analyzing the differences in the biosynthesis of their pigments and its regulation. Several studies have been conducted on the color variations in grape and demonstrated that mutations in MYB-related regulatory genes are responsible for the different color patterns (Kobayashi et al 2004(Kobayashi et al , 2005Lijavetzky et al 2006;Walker et al 2007;Giannetto et al 2008;Azuma 2009). Similarly, others have studied color mutants in apple (Dickinson and White 1986;Arakawa et al 1999;Awad et al 2000;Ben-Yehudah et al 2005), but have not clearly elucidated the genetic basis of these variations.…”

Section: Introductionmentioning

confidence: 99%

Comparison of MdMYB1 sequences and expression of anthocyanin biosynthetic and regulatory genes between Malus domestica Borkh. cultivar ‘Ralls’ and its blushed sport

Feng

Jiao

et al. 2011

Euphytica

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A mutant tree with blushed coloring pattern in fruit skin was found and identified as a bud sport of Malus domestica Borkh. cultivar 'Ralls'. This study was aimed at elucidating the genetic basis of this pattern by a comparison of 'Ralls' (striped red) with this bud sport. These two varieties were grown either under normal sunlight (control group) or the fruits were bagged during development (bagged group) and the bags were removed 1 month before harvesting. Anthocyanin content increased during fruit ripening and was higher in the blushed sport than in 'Ralls', by 57% in the control group and by 73% in the bagged group after bag removal. Correspondingly, in both the varieties transcription of six anthocyanin biosynthetic genes was coordinately up-regulated, concomitant with an increase in levels of transcripts of the regulatory gene, MdMYB1, and in general these levels were higher in the blushed sport than in ''Ralls''. However, there were no significant differences between the two varieties in the nucleotide sequences of the genomic region or coding sequence of MdMYB1; nor in the sequences of cDNAs of the other known transcription factors MdbHLH3 and MdbHLH33. Interestingly, DNA methylation in the promoter region of MdMYB1 was inversely correlated with transcript levels of MdMYB1 and was significantly lower in the blushed sport. Thus, we conclude that DNA methylation plays a role in regulating MdMYB1 expression, which in turn regulates anthocyanin biosynthesis and thereby influences the color pattern of the apples.

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“…These markers which are locus-specifi c are characterized by their hyper-variability, abundance, high reproducibility, Mendelian inheritance and co-dominant nature. They are not affected by the environmental factors (soil, climate, methods of cultivation, diseases) infl uences (Ghete et al, 2010Giannetto et al, 2008. SSRs provide highly informative markers because they are co-dominant (unlike RAPDs and AFLPs) and generally highly polymorphic.…”

Section: Analyses and Comparison Of Different Type Of Microsatellitesmentioning

confidence: 99%

Characterization of vine varieties by SSR markers

Dokupilová¹,

Šturdı́k²,

Mihálik³

2013

Acta Chimica Slovaca

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Nowadays, there are well known PCR-based techniques to detect polymorphism in plants. Marker technologies which are applied in breeding and varietal characterization can be exchanged across laboratories with standardization to yield reproducible results. This article describes applying of simple sequence repeats (SSR) markers in European laboratories, in which the reproducibility of these popular markers are examined. One of the European Union interests is characterization of grape varieties and preparation of International Vitis catalogue for comparison global varieties as Chardonnay, Sauvignon, Pinot noir with the domestic for each other country. Grape genome contains of many SSR, which are highly polymorphic. This review provides the latest information in SSR research including novels in research, comparison, development and application of microsatellites.

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A PCR-based diagnostic tool for distinguishing grape skin color mutants

Cited by 20 publications

References 23 publications

Molecular characterization of berry skin color reversion on grape somatic variants

Molecular characterization of berry skin color reversion on grape somatic variants

Comparison of MdMYB1 sequences and expression of anthocyanin biosynthetic and regulatory genes between Malus domestica Borkh. cultivar ‘Ralls’ and its blushed sport

Characterization of vine varieties by SSR markers

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