A passive portable microfluidic blood–plasma separator for simultaneous determination of direct and indirect ABO/Rh blood typing

Karimi, Shadi; Mehrdel, Pouya; Farré-Lladós, Josep; Casals‐Terré, Jasmina

doi:10.1039/c9lc00690g

Cited by 17 publications

(12 citation statements)

References 39 publications

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“…Paper-based colorimetric assays, featuring rapidity and convenience, hold great promise for point-of-care (POC) blood typing with naked-eye readouts. − However, these approaches frequently encounter inevitable environmental interference and low precision during the process of readout. , Moreover, the simultaneously grouping of multiplexed blood types such as ABO and Rhesus (Rh) always depends on high-throughput signal transduction strategies and highly cost-effective readout methods. − Our group previously invented a dye-assisted grouping methodology for rapid blood typing by identifying blood component-induced color changes after staining these components using the dye bromocresol green (BCG); we also explored a disk-based approach for multiplexed blood grouping within 2 min by using machine learning-empowered spectrometry . However, the latter still faces difficulties in dealing with field-deployable samples because spectrometers with an integrating sphere represent a heavy burden to guarantee the stability and repeatability of a result when measuring the resultant reflectance spectra, which significantly lowers the on-site feasibility.…”

Section: Introductionmentioning

confidence: 99%

Flipped Quick-Response Code Enables Reliable Blood Grouping

Zhang

Liu

et al. 2021

ACS Nano

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Accurate and rapid blood typing plays a vital role in a variety of biomedical and forensic scenarios, but recognizing weak agglutination remains challenging. Herein, we demonstrated a flipping identification with a prompt error-discrimination (FLIPPED) platform for automatic blood group readouts. Bromocresol green dye was exploited as a characteristic chromatography indicator for the differentiation of plasma from whole blood by presenting a teal color against a brown color. After integrating these color changes into a quick-response (QR) code, prompt typing of ABO and Rhesus groups was automatically achieved and data could be uploaded wirelessly within 30 s using a commercially available smartphone to facilitate blood cross-matching. We further designed a color correction model and algorithm to remove potential errors from scanning angles and ambient light intensities, by which weak agglutination could be accurately recognized. With comparable accuracy and repeatability to classical column assay in grouping 450 blood samples, the proposed approach further demonstrates to be a versatile sample-to-result platform for clinical diagnostics, food safety, and environmental monitoring.

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Section: Introductionmentioning

confidence: 99%

Flipped Quick-Response Code Enables Reliable Blood Grouping

Zhang

Liu

et al. 2021

ACS Nano

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“…PDMS and etched glass, are fabricated separately. [20] The etched glass is fabricated according to the standard wet-etching process to create a tank with the pillar array in the glass. The PDMS, on which the microchannel carved by the aid of mold, is manufactured utilizing the conventional standard soft-lithography procedure.…”

Section: Fabricationmentioning

confidence: 99%

Numerical and experimental analysis of a high-throughput blood plasma separator for point-of-care applications

et al. 2021

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Blood plasma separation from undiluted blood is an essential step in many diagnostic procedures. This study focuses on the numerical optimization of the microfluidic blood plasma separator (BPS) and experimental validation of the results to achieve portable blood plasma separation with high purity and reasonable yield. The proposed design has two parts: a microchannel for blood processing and a tank, below the aforementioned main channel, for plasma collection. The study uses 3D computational fluid dynamic analysis to investigate the optimal ratio of heights between the top microchannel and the tank and their geometry at various flow rates. Thereafter, the results are compared with the experimental findings of the fabricated devices. These results are put in contrast with some recent reported works to verify the proposed device's contribution to the improvement of quality and quantity of the extracted plasma. The optimized design is capable to achieve a 19% yield with a purity of 77.1% and depending on the requirement of the point-of-care (POC) application. These amounts could be tuned for instance to 100% pure plasma, but the yield would decrease to 9%. In this study the candidate application is hemostasis, therefore, the BPS is integrated to a biomimetic surface for hemostasis evaluation near the patients.Received: ((will be filled in by the editorial staff)) Revised: ((will be filled in by the editorial staff))

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“…With the support of the analysis of hydrodynamic effects governing blood flow by Finite Element Method based modeling, the construction of a simple quantitative device for blood group determination can be fulfilled. This proof-of-concept chip is illustrated in Based on the advantages of microfluidic devices, there is another methodology with more integration elements [12] . Here, after separating blood cells from A plug-based microfluidic device was designed to perform analyses on multiple agglutination assays in parallel without cross-contamination, using only microliter volumes of blood [13] .…”

Section: Conventional Microfluidic Devicementioning

confidence: 99%

Microchip technology applications for blood group analysis

Gao¹,

Misevic²

2020

Blood and Genomics

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Blood group analysis techniques are some of the most in demand immunological applications in clinical transfusion praxis and organ transplantation. In order to aid the advance towards higher throughput and increased sensitivity, analytical solutions dealing with a minimal amount of blood samples and the miniaturization of diagnostic equipment using microchip technologies have been evolving into an optimal solution. Here we review fabrication technologies for various types of microstructure on microchips, related operating procedures, and characterization approaches. Our focus is on examples of microchip technology and instrumentation used for blood group analysis ranging from classical serological methods of glycoprotein detection and solid phase assays, to nucleic acid amplification techniques. Molecular typing using microchip-based techniques is emerging as a supplement to standard serological methods. Microchip technology will play its key role to support blood group analysis at the molecular scale by using microliters of blood samples for extremely sensitive, quantitative, and high throughput analyses.

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A passive portable microfluidic blood–plasma separator for simultaneous determination of direct and indirect ABO/Rh blood typing

Abstract: A passive portable microfluidic blood–plasma separator for simultaneous determination of direct and indirect ABO/Rh blood typing.

Cited by 17 publications

References 39 publications

Flipped Quick-Response Code Enables Reliable Blood Grouping

Flipped Quick-Response Code Enables Reliable Blood Grouping

Numerical and experimental analysis of a high-throughput blood plasma separator for point-of-care applications

Microchip technology applications for blood group analysis

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