Abstract:Correlated neuronal activity is instrumental in the formation of networks, but its emergence during maturation is poorly understood. We have used multibeam two-photon calcium microscopy combined with targeted electrophysiological recordings in order to determine the development of population coherence from embryonic to postnatal stages in the hippocampus. At embryonic stages (E16-E19), synchronized activity is absent, and neurons are intrinsically active and generate L-type channel-mediated calcium spikes. At … Show more
“…(2) Niesner et al 2008). While the former have better signal to noise ratio and thus can get good images from deeper regions of a sample, the latter are typically much faster thus allowing real time imaging of really fast processes such as blood Xow (Niesner et al 2007) or nerve activity (Crepel et al 2007). For PMT, the positioning within the light path is an important consideration.…”
Section: Microscopementioning
confidence: 99%
“…It is very much dependent on the type of question being asked, what type of analysis is required and to what degree it can be automated. While calcium waves in neuronal networks or optical colocalizations in Wxed or non-moving tissues can be readily analyzed with existing software tools (Crepel et al 2007;Gobel et al 2007), the analysis of cell migration or cellcell contact formation is still not done properly by existing software and, in fact, is done manually or at least with heavy manual veriWcation till today (Hauser et al 2007). …”
Analyzing the dynamics of cellular immune responses, although performed for decades in immunologic research, has seen an enormous increase in the number of studies using this approach since the development of intravital 2-photon microscopy. Meanwhile, new insights into the dynamics of cellular immunity are being published on a daily basis. This review gives a short overview of the currently most widely used techniques, both on the microscopy side as well as on the experimental part. DiYculties and promises will be discussed. Finally, a personal selection of the most interesting Wndings of the Wrst 6 years of intravital 2-photon microscopy for immunological questions will be given. The overall aim is to get the reader interested into this fascinating way of investigating the immune response by means of "dynamic histology". This already has and will continue to broaden our view on how immune cells work in real life.
“…(2) Niesner et al 2008). While the former have better signal to noise ratio and thus can get good images from deeper regions of a sample, the latter are typically much faster thus allowing real time imaging of really fast processes such as blood Xow (Niesner et al 2007) or nerve activity (Crepel et al 2007). For PMT, the positioning within the light path is an important consideration.…”
Section: Microscopementioning
confidence: 99%
“…It is very much dependent on the type of question being asked, what type of analysis is required and to what degree it can be automated. While calcium waves in neuronal networks or optical colocalizations in Wxed or non-moving tissues can be readily analyzed with existing software tools (Crepel et al 2007;Gobel et al 2007), the analysis of cell migration or cellcell contact formation is still not done properly by existing software and, in fact, is done manually or at least with heavy manual veriWcation till today (Hauser et al 2007). …”
Analyzing the dynamics of cellular immune responses, although performed for decades in immunologic research, has seen an enormous increase in the number of studies using this approach since the development of intravital 2-photon microscopy. Meanwhile, new insights into the dynamics of cellular immunity are being published on a daily basis. This review gives a short overview of the currently most widely used techniques, both on the microscopy side as well as on the experimental part. DiYculties and promises will be discussed. Finally, a personal selection of the most interesting Wndings of the Wrst 6 years of intravital 2-photon microscopy for immunological questions will be given. The overall aim is to get the reader interested into this fascinating way of investigating the immune response by means of "dynamic histology". This already has and will continue to broaden our view on how immune cells work in real life.
“…Esta característica é originada por propriedades biofísicas da membrana de neurônios imaturos que acarreta no desencadeamento de eventos esporádicos de aumento transitório da concentração de cálcio intracelular. Sendo assim, a atividade elétrico/química dos neurônios promove mudanças no meio onde está, influenciando a ativação/inativação de programas genéticos específicos (Ben-Ari, 2001, Crepel, et al, 2007, Blankenship ,Feller, 2010. Os GDPs são propagados através de rede de interações entre neurônios, que abrange virtualmente todo hipocampo (região CA1 e CA3 e hilo) durante a primeira e segunda semana do desenvolvimento pós-natal de roedores (P0-P10).…”
Section: Desenvolvimento Pós-natal Do Hipocampo De Roedoresunclassified
“…(A4) Três tipos de eventos podem ser discriminadas, como mostrado pela sua representação de traços gerados a partir de variações na fluorescência (preto, picos de cálcio; vermelho, platôs de cálcio; azul, GDPs). (B1) Current-clamp simultâneo (V repouso: ~-60 mV, topo) e registros ópticos (traço abaixo) em três neurônios representativos para os três tipos de atividade de cálcio descritos em A. Adaptada de Crepel et al (2007) O surgimento dos padrões de atividade espontâneos sincronizados é desencadeado pelo início da formação da circuitaria hipocampal, definida pelo estabelecimento e refinamento da comunicação entre os neurônios. Este episódio auxilia a determinar as alterações neuronais que contribuem para o desenvolvimento pós-natal do hipocampo.…”
Section: Figura 4-caracterização Por Microscopia Multifóton De Três Dunclassified
“…Apesar desses estudos evidenciarem a importância deste tipo de comunicação para o estabelecimento da atividade sincronizada, ainda não há trabalhos sobre o papel de Cxs específicas e sua contribuição individual para modulação desses eventos (Strata et al, 1997, Crepel et al, 2007, Molchanova et al, 2016.…”
Section: Jcs Durante O Desenvolvimento Do Sistema Nervoso De Roedoresunclassified
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