A Palm Germ-Radar (PaGeR) for rapid and simple COVID-19 detection by reverse transcription loop-mediated isothermal amplification (RT-LAMP)

Abstract

The current COVID-19 pandemic caused by SARS-CoV-2 is raging, seriously threatening people's lives. The establishment of rapid and accurate pathogen detection technology is not only critical in this epidemic, but also a reminder that we must always be prepared for possible future outbreaks. Therefore, we developed a Palm Germ-Radar (PaGeR) device for rapid and simple detection of COVID-19 from extracted patient sample RNA by RT-LAMP. The whole procedure of rapid COVID-19 detection is based on 4 simple steps: inactivation, extraction, amplification, and detection. SARS-CoV-2 down to 1 copy/μL could be detected selectively with naked-eye. Three detection methods (colorimetric, fluorometric and lateral dipstick readout) could be performed in PaGeR instrument. By employing the PaGeR, we successfully detected SARS-CoV-2 in clinical RNA samples isolated from swab specimens. The results showed that 15 out of 17 COVID-19 patients were diagnosed as positive while all 55 normal samples were diagnosed as negative. Therefore, the developed PaGeR instrument can realize the detection of COVID-19 with easily visualized results, providing a promising instrument for rapid detection in the community as well as at home.

Current and Perspective Sensing Methods for Monkeypox Virus

“…For instance, the IoT-based detection of COVID-19 using LAMP technology has been demonstrated with satisfactory performance [ 81 ]. Similarly, another field-deployable RT-LAMP-based device for onsite virus inactivation and detection was also reported [ 82 ]. These advanced approaches can be extended to MPXV diagnostics.…”

Electrochemical paper-based antigen sensing platform using plant-derived monoclonal antibody for detecting SARS-CoV-2

“…The outbreak has therefore begun to enable diagnostic assays for screening and preventing the spread of the disease. Current diagnostic methods have solely relied on the nucleic acid amplification technologies, such as real time - polymerase chain reaction (RT-PCR) [ [4] , [5] , [6] ] and Loop mediated isothermal amplification (LAMP) [ [7] , [8] , [9] ]. Nevertheless, these methods have merits, such as they require expensive instruments and sophisticated operation steps which are the key bottlenecks in testing that dramatically increases cost and assay time from sample-to-answer.…”

Electromechanical RT-LAMP device for portable SARS-CoV-2 detection

“…The device could detect SARS-CoV-2 in 25 min with a limit of detection of 10 5 copies in 25 μL sample. Based on RT-LAMP, the Palm Germ-Radar (PaGeR) platform can detect the COVID-19 viral genome down to 1-5 ×10 3 copies/mL within 1 h with a naked-eye using three detection methods, which were colorimetric, fluorimetric, and lateral dipstick [ 12 ]. However, the platform could not enable real-time in situ quantitative monitoring.…”