A one-step potentiometric immunoassay for plasma cardiac troponin I using an antibody-functionalized bis-MPA–COOH dendrimer as a competitor with improved sensitivity

Ni, Erru; Fang, Yizhen; Ma, Fangfang; Ge, Gaoshun; Wu, Jingyi; Wang, Yingying; Lin, Yao; Xie, Huabin

doi:10.1039/d0ay00680g

Cited by 8 publications

(3 citation statements)

References 46 publications

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“…(B) Schematic illustration of potentiometric immunoassay for one-step detection of cTnI on cTnI-modified screen-printed graphite electrode electrodeposited with AuNPs. Reproduced with permission from ref . Copyright 2020 The Royal Society of Chemistry.…”

Section: Surface Immobilization and Signalingmentioning

confidence: 99%

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Progress, Opportunities, and Challenges of Troponin Analysis in the Early Diagnosis of Cardiovascular Diseases

Zhang

et al. 2021

Anal. Chem.

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Section: Surface Immobilization and Signalingmentioning

confidence: 99%

“…For example, Ni et al report a new one-step potentiometric immunoassay for one-step determination of cTnI (Figure B) . Specifically, cTnI is immobilized on the gold nanoparticle-modified SPGE substrate.…”

Section: Surface Immobilization and Signalingmentioning

confidence: 99%

Progress, Opportunities, and Challenges of Troponin Analysis in the Early Diagnosis of Cardiovascular Diseases

Zhang

et al. 2021

Anal. Chem.

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“…More recently, the multivalency of dendrimers has attracted research interest for the fabrication of dendrimer-based biosensors for biomarker detection. The multiple functional groups on the surface of dendrimers are used to immobilize antibodies or aptamers combined with highly sensitive electrochemical or fluorescence methods for specific tumor biomarker detection [ 30 , 31 , 32 , 33 , 34 , 35 , 36 ]. However, the use of dendrimers for biomarker detection purposes can also be figured out through the direct interaction of dendrimers with proteins and biomolecules in a biological fluid, thus approaching the principles of liquid biopsy mentioned above.…”

Section: Introductionmentioning

confidence: 99%

Fluorescence Liquid Biopsy for Cancer Detection Is Improved by Using Cationic Dendronized Hyperbranched Polymer

Morcuende-Ventura

Hermoso-Durán

Abian-Franco

et al. 2021

IJMS

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(1) Background: Biophysical techniques applied to serum samples characterization could promote the development of new diagnostic tools. Fluorescence spectroscopy has been previously applied to biological samples from cancer patients and differences from healthy individuals were observed. Dendronized hyperbranched polymers (DHP) based on bis(hydroxymethyl)propionic acid (bis-MPA) were developed in our group and their potential biomedical applications explored. (2) Methods: A total of 94 serum samples from diagnosed cancer patients and healthy individuals were studied (20 pancreatic ductal adenocarcinoma, 25 blood donor, 24 ovarian cancer, and 25 benign ovarian cyst samples). (3) Results: Fluorescence spectra of serum samples (fluorescence liquid biopsy, FLB) in the presence and the absence of DHP-bMPA were recorded and two parameters from the signal curves obtained. A secondary parameter, the fluorescence spectrum score (FSscore), was calculated, and the diagnostic model assessed. For pancreatic ductal adenocarcinoma (PDAC) and ovarian cancer, the classification performance was improved when including DHP-bMPA, achieving high values of statistical sensitivity and specificity (over 85% for both pathologies). (4) Conclusions: We have applied FLB as a quick, simple, and minimally invasive promising technique in cancer diagnosis. The classification performance of the diagnostic method was further improved by using DHP-bMPA, which interacted differentially with serum samples from healthy and diseased subjects. These preliminary results set the basis for a larger study and move FLB closer to its clinical application, providing useful information for the oncologist during patient diagnosis.

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Potentiometric Biosensors

Fatibello‐Filho

2021

Tools and Trends in Bioanalytical Chemistry

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A one-step potentiometric immunoassay for plasma cardiac troponin I using an antibody-functionalized bis-MPA–COOH dendrimer as a competitor with improved sensitivity

Abstract: Herein, we have reported a new one-step potentiometric immunoassay for the sensitive and specific detection of human plasma cardiac troponin I (cTnI), a biomarker of cardio-cerebrovascular diseases.

Cited by 8 publications

References 46 publications

Progress, Opportunities, and Challenges of Troponin Analysis in the Early Diagnosis of Cardiovascular Diseases

Progress, Opportunities, and Challenges of Troponin Analysis in the Early Diagnosis of Cardiovascular Diseases

Fluorescence Liquid Biopsy for Cancer Detection Is Improved by Using Cationic Dendronized Hyperbranched Polymer

Potentiometric Biosensors

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