A one-step method to construct a third-generation biosensor based on horseradish peroxidase and gold nanoparticles embedded in silica sol–gel network on gold modified electrode

Di, Junwei; Shen, Chunping; Peng, Shaohua; Tu, Yifeng; Li, Shujin

doi:10.1016/j.aca.2005.08.013

Cited by 67 publications

(29 citation statements)

References 23 publications

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“…Xiao et al developed a biosensor based on HRP immobilized on nano-Au. However, the lifetime of the biosensor was not satisfied [17]. Additionally, SiO 2 nanoparticles have also been found to be a good biocompatible solid support for biomolecule immobilization to construct biosensor [11,12].…”

Section: Introductionmentioning

confidence: 99%

“…Additionally, SiO 2 nanoparticles have also been found to be a good biocompatible solid support for biomolecule immobilization to construct biosensor [11,12]. Although the lifetime of the biosensor based on HRP immobilized on SiO 2 nanoparticles was in the range of several months, the conductivity was poor, which resulted in the low sensitivity [17]. Actually, the composite nanomaterials have a synergistic effect of each component [18].…”

Section: Introductionmentioning

confidence: 99%

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Enzyme biosensor based on the immobilization of HRP on SiO2/BSA/Au composite nanoparticles

Yuan

Zhuo

et al. 2010

Appl Biochem Biotechnol

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In this work, an enzyme biosensor based on the immobilization of horseradish peroxidase (HRP) on SiO₂/BSA/Au/thionine/nafion-modified gold electrode was fabricated successfully. Firstly, nafion was dropped on the surface of the gold electrode to form a nafion film followed by chemisorption of thionine (Thi) as an electron mediator via the ion-exchange interaction between the Thi and nafion. Subsequently, the SiO₂/BSA/Au composite nanoparticles were assembled onto Thi film through the covalent bounding with the amino groups of Thi. Finally, HRP was immobilized on the SiO₂/BSA/Au composite nanoparticles due to the covalent conjugation to construct an enzyme biosensor. The surface topographies of the SiO₂/BSA/Au composite nanoparticles were investigated by using scanning electronic microscopy. The stepwise self-assemble procedure of the biosensor was further characterized by means of cyclic voltammetry and chronoamperometry. The enzyme biosensor showed high sensitivity, good stability and selectivity, a wide linear response to hydrogen peroxide (H₂O₂) in the range of 8.0 x 10⁻⁶ ~3.72 x 10⁻³ mol/L, with a detection limit of 2.0 x 10⁻⁶ mol/L. The Michaelies-Menten constant K(app)(M) value was estimated to be 2.3 mM.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Enzyme biosensor based on the immobilization of HRP on SiO2/BSA/Au composite nanoparticles

Yuan

Zhuo

et al. 2010

Appl Biochem Biotechnol

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“…Gold nanoparticles (AuNPs) are used increasingly in many electrochemical applications since they have the ability to enhance the electrode conductivity and facilitate the electron transfer, thus, improving the analytical selectivity and sensitivity. Especially the small size and biocompatible ability of AuNPs allows them utilized widely as a base in modification of various biosensors to promote the direct electron transfer (DET) of some proteins such as horseradish peroxidase [9,10], hemoglobin [11], myoglobin [12], glucose oxidase [13] and superoxide dismutase [14].…”

Section: Introductionmentioning

confidence: 99%

Fabrication of Nanocomposite Containing Naphthoquinone and Nanogold Supported on Poly(2,6‐pyridinedicarboxylic acid) Film for Voltammetric Determination of N‐Acetyl‐L‐Cysteine

et al. 2009

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A modified electrode was fabricated by grafting of poly (2,6-pyridinedicarboxylic acid) film (PDC) by electropolymerization of 2,6-pyridinedicarboxylic acid on the glassy carbon electrode (GCE). Then, gold nanoparticles (NG) and 1,2-naphthoquinone-4-sulfonic acid sodium (Nq) were immobilized on the PDC/GCE to prepare Nq/NG/PDC/ GCE by immersing electrode into NG and Nq solution, respectively. The Nq species on NG/PDC/GCE could catalyze electrooxidation of N-acetyl-l-cysteine (NAC) with lowering the over potential by about 600 mV. This method used for detection of NAC in dynamic range from 4.0 Â 10 À6 M to 1.30 Â 10 À4 M with a detection of limit (2s) 8.0 Â 10 À7 M.

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“…Several of amperometric biosensors were developed based on CNT and mediators [13][14][15][16]. However, it not only inconvenient to add a mediator in the fabrication process, but also will reduce the reaction selectivity as redox mediators are rather general catalysts [23]. Zhao et al [17] reported the direct electrochemistry of HRP at CNT powder microelectrode.…”

Section: Introductionmentioning

confidence: 99%

A carbon nanotube/silica sol–gel architecture for immobilization of horseradish peroxidase for electrochemical biosensor

Wang¹,

Gu²,

Di³

et al. 2007

Bioprocess Biosyst Eng

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A novel third-generation biosensor for hydrogen peroxide (H202) has been constructed based on horseradish peroxidase (HRP) immobilized by the sol-gel (SG) technology on carbon nanotube (CNT)-modified electrode. CNT has good promotion effects on the direct electron transfer between HRP and the electrode surface and the SG network provides a biocompatible microenvironment for enzyme. The immobilized HRP retained its bioelectrocatalytic activity for the reduction of hydrogen peroxide and can respond to the change of concentration of H2O2 rapidly. The heterogeneous electron transfer rate constant was evaluated to be 2.8 +/- 0.4 s(-1). The amperometric response to H2O2 shows a linear relation in the range from 0.5 to 300 micromol 1(-1) and a detection limit of 0.1 micromol 1(-1) (S/N = 3). The KMapp value of HRP immobilized on the electrode surface was found to be 1.35 mmol 1(-1). The biosensor exhibited high sensitivity, rapid response and excellent long-term stability.

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A one-step method to construct a third-generation biosensor based on horseradish peroxidase and gold nanoparticles embedded in silica sol–gel network on gold modified electrode

Cited by 67 publications

References 23 publications

Enzyme biosensor based on the immobilization of HRP on SiO2/BSA/Au composite nanoparticles

Enzyme biosensor based on the immobilization of HRP on SiO2/BSA/Au composite nanoparticles

Fabrication of Nanocomposite Containing Naphthoquinone and Nanogold Supported on Poly(2,6‐pyridinedicarboxylic acid) Film for Voltammetric Determination of N‐Acetyl‐L‐Cysteine

A carbon nanotube/silica sol–gel architecture for immobilization of horseradish peroxidase for electrochemical biosensor

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