A nuclear encoded chloroplast ATP synthase mutant of Zea mays L

Echt, Craig S.; Polacco, Mary L.; Neuffer, M. G.

doi:10.1007/bf00330447

Cited by 9 publications

(8 citation statements)

References 20 publications

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“…Furthermore, all of the maize mutants described previously as lacking specifically ATP synthase activity (Echt et al, 1987;Miles, 1994) as well as all of the ATP synthase mutants newly isolated in our laboratory are deficient for both membrane intrinsic (CF 0 ) and membrane extrinsic (CF,) coupling factor subunits, indicating that these two subcomplexes accumulate coordinately (Figure 2 and data not shown). We cannot eliminate the possibility, however, that crsl functions in another aspect of ATP synthase biogenesis in addition to its role in atpF RNA splicing.…”

Section: Resultsmentioning

confidence: 97%

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Nuclear mutations that block group II RNA splicing in maize chloroplasts reveal several intron classes with distinct requirements for splicing factors.

1997

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To elucidate mechanisms that regulate chloroplast RNA splicing in multicellular plants, we sought nuclear mutations in maize that result in chloroplast splicing defects. Evidence is presented for two nuclear genes whose function is required for the splicing of group II introns in maize chloroplasts. A mutation in the crsl (for chloroplast RNA gplicing 1) gene blocks the splicing of only the atpF intron, whereas a mutation in the crs2 gene blocks the splicing of many chloroplast introns. In addition, a correlation was observed between the absence of plastid ribosomes and the failure to splice several chloroplast introns. Our results suggest that a chloroplast-encoded factor and a nuclear-encoded factor whose activity requires crs2 function facilitate the splicing of distinct sets of group II introns. These two genetically defined intron sets also differ with regard to intron structure: one set consists of only subgroup IIA introns and the other of only subgroup IIB introns. Therefore, it is likely that distinct splicing factors recognize subgroup-specific features of intron structure or facilitate subgroup-specific aspects of the splicing reaction. Of the 12 pre-mRNA introns in the maize chloroplast genome, only one is normally spliced in both crs2 mutants and in mutants lacking plastid ribosomes, indicating that few, if any, of the group II introns in the chloroplast genome undergo autocatalytic splicing in vivo.

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Section: Resultsmentioning

confidence: 97%

“…hcfl08 and cfr are nuclear mutants that were isolated and described by Echt et al (1987) and Miles (1994), respectively. These are not allelic to one another or to crsl (data not shown).…”

Section: Plant Materialsmentioning

confidence: 99%

Nuclear mutations that block group II RNA splicing in maize chloroplasts reveal several intron classes with distinct requirements for splicing factors.

1997

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“…The Fo portion forms a transmembrane ion channel for the translocation of protons (Echt et al, 1987). In this study, spot 6 and 7 were identified as the ATPS␦ chain, which were markedly up-regulated 2.3-fold and 4.2-fold at 20 d of salt treatment.…”

Section: Energy Metabolismmentioning

confidence: 99%

Identification of differentially expressed salt-responsive proteins in roots of two perennial grass species contrasting in salinity tolerance

Liu

XiaoXia

et al. 2012

Journal of Plant Physiology

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“…Biochemical analysis indicates that many hcfmutations in maize primarily affect a single chloroplast protein complex. These mutations may lie in genes encoding structural components of these complexes (Miles, 1982;Barkan et al, 1986;Echt et al, 1987). However, some hcf mutations affect several complexes, suggesting that they disrupt the regulation of a set of genes, or that they disrupt common mechanisms of synthesis or assembly.…”

Section: Introductionmentioning

confidence: 99%

Molecular cloning of a maize gene involved in photosynthetic membrane organization that is regulated by Robertson's Mutator.

et al. 1989

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The maize photosynthetic mutant hcf106 has a distinctive and unusual thylakoid membrane organization, and fails to accumulate three of the five thylakoid membrane protein complexes. This mutant arose in a Robertson's Mutator background, and shows somatic instability typical of a transposon‐induced allele. In addition, hcf106 is suppressed when Mu1 elements are inactive and modified in their terminal inverted repeats. Thus plants homozygous for the mutant allele adopt a mutant phenotype only when Mu1 elements are active and unmodified. DNA from the mutant allele has been cloned by ‘transposon‐tagging’ using the transposon Mu1, and the identity of the clone confirmed by observing somatic excision of the transposon in a revertant sector. A 1.2 kb transcript homologous to the cloned DNA is found in wild‐type and suppressed seedlings, but is not found in mutant seedlings, suggesting that suppression is mediated at the level of transcript accumulation.

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A nuclear encoded chloroplast ATP synthase mutant of Zea mays L

Cited by 9 publications

References 20 publications

Nuclear mutations that block group II RNA splicing in maize chloroplasts reveal several intron classes with distinct requirements for splicing factors.

Nuclear mutations that block group II RNA splicing in maize chloroplasts reveal several intron classes with distinct requirements for splicing factors.

Identification of differentially expressed salt-responsive proteins in roots of two perennial grass species contrasting in salinity tolerance

Molecular cloning of a maize gene involved in photosynthetic membrane organization that is regulated by Robertson's Mutator.

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