A novel workflow control system for Fourier transform ion cyclotron resonance mass spectrometry allows for unique on‐the‐fly data‐dependent decisions

Taban, Ioana M.; Burgt, Yuri E. M. van der; Duursma, Marc C.; Takáts, Zoltán; Seynen, Marco; Konijnenburg, Marco; Vijftigschild, A; Attema, I.; Heeren, Ron M. A.

doi:10.1002/rcm.3440

Cited by 13 publications

(16 citation statements)

References 53 publications

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“…The stage has also been integrated into other available system control platforms, such as the workflow based AWG3 suite. 24,27 A screenshot of the AWG3 GUI stage control for a MS imaging experiment is shown in Fig. 4(b).…”

Section: Resultsmentioning

confidence: 99%

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Vacuum compatible sample positioning device for matrix assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry imaging

Aizikov

Smith

Chargin

et al. 2011

Review of Scientific Instruments

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The high mass accuracy and resolving power of Fourier transform ion cyclotron resonance mass spectrometers (FT-ICR MS) make them ideal mass detectors for mass spectrometry imaging (MSI), promising to provide unmatched molecular resolution capabilities. The intrinsic low tolerance of FT-ICR MS to RF interference, however, along with typically vertical positioning of the sample, and MSI acquisition speed requirements present numerous engineering challenges in creating robotics capable of achieving the spatial resolution to match. This work discusses a two-dimensional positioning stage designed to address these issues. The stage is capable of operating in ∼1 × 10 -8 mbar vacuum. The range of motion is set to 100 mm × 100 mm to accommodate large samples, while the positioning accuracy is demonstrated to be less than 0.4 micron in both directions under vertical load over the entire range. This device was integrated into three different matrix assisted laser desorption/ionization (MALDI) FT-ICR instruments and showed no detectable RF noise. The "oversampling" MALDI-MSI experiments, under which the sample is completely ablated at each position, followed by the target movement of the distance smaller than the laser beam, conducted on the custom-built 7T FT-ICR MS demonstrate the stability and positional accuracy of the stage robotics which delivers high spatial resolution mass spectral images at a fraction of the laser spot diameter.

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Section: Resultsmentioning

confidence: 99%

“…An AWG3 workflow-based data station is used for experiment control, data acquisition, and analysis. 24 Images were produced via an in-house developed software and visualized as "datacubes." 25…”

Section: Methodsmentioning

confidence: 99%

Vacuum compatible sample positioning device for matrix assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry imaging

Aizikov

Smith

Chargin

et al. 2011

Review of Scientific Instruments

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“…The experiment is controlled by a modular FT-ICR data acquisition system developed by Heeren and co-workers [61], which is used to control the voltages and timing of the ion source and transfer optics and ion manipulation in the ICR cell. Fig.…”

Section: Methodsmentioning

confidence: 99%

New approach for studying slow fragmentation kinetics in FT-ICR: Surface-induced dissociation combined with resonant ejection

Laskin

2015

International Journal of Mass Spectrometry

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A B S T R A C TWe introduce a new approach for studying the kinetics of large ion fragmentation in the gas phase by coupling surface-induced dissociation (SID) in a Fourier transform ion cyclotron resonance mass spectrometer with resonant ejection of selected fragment ions using a relatively short (5 ms) ejection pulse. The approach is demonstrated for singly protonated angiotensin III ions excited by collisions with a self-assembled monolayer of alkylthiol on gold (HSAM). The overall decomposition rate and rate constants of individual reaction channels are controlled by varying the kinetic energy of the precursor ion in a range of 65-95 eV. The kinetics of peptide fragmentation are probed by varying the delay time between ion activation by collision and short (5 ms) resonant ejection of selected fragment ions at a constant total reaction time of 150 ms. RRKM modeling indicates that the shape of the kinetics plots is strongly affected by the shape and position of the energy deposition function (EDF) describing the internal energy distribution of the ion following ion-surface collision. Modeling of the kinetics data provides detailed information on the shape of the EDF and energy and entropy effects of individual reaction channels.

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“…The instrument can be equipped with either a capacitively coupled cylindrical open ICR cell [53] or a Bruker Infinity cell [65], where ions are cooled with an argon pulse gas [66] before broadband frequency (chirp) excitation [67] and direct-mode detection. An in-house developed workflow-based data station (“AWG3”) is used for experiment control and data acquisition [51, 52]. …”

Section: Methodsmentioning

confidence: 99%

“…The capabilities of this custom-built instrument—such as workflow-based control software [51, 52], easy implementation of different ICR cell designs [53], and fragmentation by simultaneous electron-capture dissociation infrared multiphoton dissociation (ECD/IRMPD) [54]—expand the possibilities of FT-ICR MS imaging; such possibilities are otherwise not easily implemented on commercial systems.…”

Section: Introductionmentioning

confidence: 99%

An External Matrix-Assisted Laser Desorption Ionization Source for Flexible FT-ICR Mass Spectrometry Imaging with Internal Calibration on Adjacent Samples

Smith

Aizikov

Duursma

et al. 2011

J. Am. Soc. Mass Spectrom.

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We describe the construction and application of a new MALDI source for FT-ICR mass spectrometry imaging. The source includes a translational X-Y positioning stage with a 10 × 10 cm range of motion for analysis of large sample areas, a quadrupole for mass selection, and an external octopole ion trap with electrodes for the application of an axial potential gradient for controlled ion ejection. An off-line LC MALDI MS/MS run demonstrates the utility of the new source for data- and position-dependent experiments. A FT-ICR MS imaging experiment of a coronal rat brain section yields ∼200 unique peaks from m/z 400–1100 with corresponding mass-selected images. Mass spectra from every pixel are internally calibrated with respect to polymer calibrants collected from an adjacent slide.

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A novel workflow control system for Fourier transform ion cyclotron resonance mass spectrometry allows for unique on‐the‐fly data‐dependent decisions

Cited by 13 publications

References 53 publications

Vacuum compatible sample positioning device for matrix assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry imaging

Vacuum compatible sample positioning device for matrix assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry imaging

New approach for studying slow fragmentation kinetics in FT-ICR: Surface-induced dissociation combined with resonant ejection

An External Matrix-Assisted Laser Desorption Ionization Source for Flexible FT-ICR Mass Spectrometry Imaging with Internal Calibration on Adjacent Samples

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