A Novel Whole Yeast-Based Subunit Oral Vaccine Against Eimeria tenella in Chickens

Soutter, Francesca; Werling, Dirk; Nolan, Matthew J.; Küster, Tatiana; Attree, Elizabeth; Marugán-Hernández, Virginia; Kim, Sung-Won; Tomley, Fiona M.; Blake, Damer P.

doi:10.3389/fimmu.2022.809711

Cited by 15 publications

(14 citation statements)

References 44 publications

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“…S. cerevisiae has been one of the most popular heterologous expression hosts in biotechnology, and it is increasingly employed in oral vaccine development [ 10 – 16 , 37 – 41 ]. Our previous study [ 13 ] showed that the expression of LTB fused with a synthetic Dengue tetravalent antigen using S. cerevisiae was potentially useful as an oral vaccine against Dengue viruses.…”

Section: Discussionmentioning

confidence: 99%

Improving expression and assembly of difficult-to-express heterologous proteins in Saccharomyces cerevisiae by culturing at a sub-physiological temperature

Lương

et al. 2023

Microb Cell Fact

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Background Escherichia coli heat labile toxin B subunit (LTB) is one of the most popular oral vaccine adjuvants and intestine adsorption enhancers. It is often expressed as a fusion partner with target antigens to enhance their immunogenicity as well as gut absorbability. However, high expression levels of a fusion protein are critical to the outcome of immunization experiments and the success of subsequent vaccine development efforts. In order to improve the expression and functional assembly of LTB-fusion proteins using Saccharomyces cerevisiae, we compared their expression under culture conditions at a sub-physiological temperature 20 °C with their expression under a standard 30 °C. Results The assembled expression of LTB-EDIII2 (LTB fused to the envelope domain III (EDIII) of Dengue virus serotype 2), which was expressed at the level of 20 µg/L in our previous study, was higher when the expression temperature was 20 °C as opposed to 30 °C. We also tested whether the expression and functional assembly of a difficult-to-express LTB fusion protein could be increased. The assembled expression of the difficult-to-express LTB-VP1 fusion protein (LTB fused to VP1 antigen of Foot-and-Mouth Disease Virus) dramatically increased, although the total amount of expressed protein was still lower than that of LTB-EDIII2. Slight but significant increase in the expression of well-known reporter protein eGFP, which has previously been shown to be increased by cultivation at 20 °C, was also observed in our expression system. As no significant changes in corresponding transcripts levels and cell growth were observed between 20 °C and 30 °C, we infer that translation and post-translational assembly are responsible for these enhancements. Conclusions The effects of lowering the expression temperature from 30 °C to 20 °C on protein expression and folding levels in S. cerevisiae, using several proteins as models, are reported. When heterologous proteins are expressed at 20 °C, a greater amount of (specially, more assembled) functional proteins accumulated than at 30 °C. Although further studies are required to understand the molecular mechanisms, our results suggest that lowering the expression temperature is a convenient strategy for improving the expression of relatively complexly structured and difficult-to-express proteins in S. cerevisiae.

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Section: Discussionmentioning

confidence: 99%

Improving expression and assembly of difficult-to-express heterologous proteins in Saccharomyces cerevisiae by culturing at a sub-physiological temperature

Lương

et al. 2023

Microb Cell Fact

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“…Yeasts as eukaryotic expression systems might be preferable to bacterial vectors as indicated by the result that yeast expressed antigen provided better protection after parenteral application than the same antigen expressed in Escherichia coli (107). Live and inactivated yeast-based recombinant vaccines induced immunity after oral application (66,108). Maybe not surprisingly, wing-web application of fowl pox virus expressing an E. tenella antigen only induced unsatisfactory protection (109).…”

Section: Coccidiosismentioning

confidence: 99%

Vaccination Against Poultry Parasites

Hauck,

Macklin

2023

Avian Diseases

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The complexity of parasites and their life cycles makes vaccination against parasitic diseases challenging. This review highlights this by discussing vaccination against four relevant parasites of poultry. Coccidia, i.e., Eimeria spp., are the most important parasites in poultry production, causing multiple billions of dollars of damage worldwide. Due to the trend of antibiotic-free broiler production, use of anticoccidia vaccines in broilers is becoming much more important. As of now, only live vaccines are on the market, almost all of which must be produced in birds. In addition, these live vaccines require extra care in the management of flocks to provide adequate protection and prevent the vaccines from causing damage. Considerable efforts to develop recombinant vaccines and related work to understand the immune response against coccidia have not yet resulted in an alternative. Leucozytozoon caulleryi is a blood parasite that is prevalent in East and South Asia. It is the only poultry parasite for which a recombinant vaccine has been developed and brought to market. Histomonas meleagridis causes typhlohepatitis in chickens and turkeys. The systemic immune response after intramuscular vaccination with inactivated parasites is not protective. The parasite can be grown and attenuated in vitro, but only together with bacteria. This and the necessary intracloacal application make the use of live vaccines difficult. So far, there have been no attempts to develop a recombinant vaccine against H. meleagridis. Inactivated vaccines inducing antibodies against the poultry red mite Dermanyssus gallinae have the potential to control infestations with this parasite. Potential antigens for recombinant vaccines have been identified, but the use of wholemite extracts yields superior results. In conclusion, while every parasite is unique, development of vaccines against them shares common problems, namely the difficulties of propagating them in vitro and the identification of protective antigens that might be used in recombinant vaccines.RESUMEN. Estudio recapitulativo-Vacunación contra los parásitos de las aves de corral. La complejidad de los parásitos y sus ciclos de vida hace que la vacunación contra las enfermedades parasitarias sea un desafı o. Esta revisión destaca este concepto al discutir la vacunación contra cuatro parásitos relevantes en la avicultura. Las coccidias, como, Eimeria spp., son los parásitos más importantes en la producción avı cola y causan daños por miles de millones de dólares en todo el mundo. Debido a la tendencia de la producción de pollos de engorde sin antibióticos, el uso de vacunas anticoccidianas en pollos de engorde se está volviendo mucho más importante. Por el momento, sólo hay en el mercado vacunas vivas y casi todas ellas deben producirse en aves. Además, estas vacunas vivas requieren un cuidado especial en el manejo de las parvadas para brindar una protección adecuada y evitar que las vacunas causen daños. Los esfuerzos considerables para desarrollar vacunas recombinantes y los trabajos...

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“…However, there is a knowledge gap in the comparative efficacy of different plant-derived adjuvants against various Eimeria strains. Recently, an oral, yeast-based sub-unit vaccine for E. tenella has been shown to decrease Eimeria replication (Soutter et al 2022 ). Nano-vaccine adjuvants in apicomplexan vaccines can offer a sustained immune protection, compared to other adjuvants.…”

Section: Eimeria Vaccination Challenges and Other Alternativ...mentioning

confidence: 99%

Vaccines against chicken coccidiosis with particular reference to previous decade: progress, challenges, and opportunities

et al. 2022

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Chicken coccidiosis is an economically significant disease of commercial chicken industry accounting for losses of more than £10.4 billion (according to 2016 prices). Additionally, the costs incurred in prophylaxis and therapeutics against chicken coccidiosis in developing countries (for instance Pakistan according to 2018 prices) reached US $45,000.00 while production losses for various categories of chicken ranges 104.74 to US $2,750,779.00. The infection has been reported from all types of commercial chickens (broiler, layer, breeder) having a range of reported prevalence of 7–90%. The concern of resistance towards major anticoccidials has provided a way forward to vaccine research and development. For prophylaxis of chicken coccidiosis, live virulent, attenuated, ionophore tolerant strains and recombinant vaccines have been extensively trialed and commercialized. Eimeria antigens and novel vaccine adjuvants have elicited the protective efficacy against coccidial challenge. The cost of production and achieving robust immune responses in birds are major challenges for commercial vaccine production. In the future, research should be focused on the development of multivalent anticoccidial vaccines for commercial poultry. Efforts should also be made on the discovery of novel antigens for incorporation into vaccine designs which might be more effective against multiple Eimeria species. This review presents a recap to the overall progress against chicken Eimeria with particular reference to previous decade. The article presents critical analysis of potential areas for future research in chicken Eimeria vaccine development.

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A Novel Whole Yeast-Based Subunit Oral Vaccine Against Eimeria tenella in Chickens

Cited by 15 publications

References 44 publications

Improving expression and assembly of difficult-to-express heterologous proteins in Saccharomyces cerevisiae by culturing at a sub-physiological temperature

Improving expression and assembly of difficult-to-express heterologous proteins in Saccharomyces cerevisiae by culturing at a sub-physiological temperature

Vaccination Against Poultry Parasites

Vaccines against chicken coccidiosis with particular reference to previous decade: progress, challenges, and opportunities

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