A novel VS<sub>2</sub>nanosheet-based biosensor for rapid fluorescence detection of cytochrome c

Yin, Xuehua; Cai, Jin; Feng, Hongyan; Wu, Zeming; Zou, Jianmei; Cai, Qingyun

doi:10.1039/c4nj01971g

Cited by 40 publications

(22 citation statements)

References 59 publications

(54 reference statements)

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“…Taking advantage of exonuclease III‐aided DNA recycling amplification and terminal protection of small‐molecule‐linked approach, this biosensor successfully converted protein detection into DNA analysis with a LOD of SA down to 0.67 ng mL −1 . Yin et al combined aptamer‐based bioassay with VS 2 NSs to fabricate an ultrasensitive biosensor for detection of cytochrome c (Cyt c) . The proposed sensing system has high sensitivity and selectivity with a LOD down to 0.5 n M and a linear range from 0.75 n M to 50 µ M .…”

Section: Biosensors Based On 2d Tmdc Nssmentioning

confidence: 99%

Recent Advances in Synthesis and Biomedical Applications of Two‐Dimensional Transition Metal Dichalcogenide Nanosheets

Shan

Zhang

et al. 2016

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During recent decades, a giant leap in the development of nanotechnology has been witnessed. Numerous nanomaterials with different dimensions and unprecedented features have been developed and provided unimaginably wide scope to solve the challenging problems in biomedicine, such as cancer diagnosis and therapy. Recently, two-dimensional (2D) transition metal dichalcogenide (TMDC) nanosheets (NSs), including MoS , WS , and etc., have emerged as novel inorganic graphene analogues and attracted tremendous attention due to their unique structures and distinctive properties, and opened up great opportunities for biomedical applications, including ultrasensitive biosensing, biological imaging, drug delivery, cancer therapy, and antibacterial treatment. A comprehensive overview of different synthetic methods of ultrathin 2D TMDC NSs and their state-of-the-art biomedical applications, especially those that have appeared in the past few years, is presented. At the end of this review, the future opportunities and challenges for 2D TMDC NSs in biomedicine are also discussed.

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Section: Biosensors Based On 2d Tmdc Nssmentioning

confidence: 99%

Recent Advances in Synthesis and Biomedical Applications of Two‐Dimensional Transition Metal Dichalcogenide Nanosheets

Shan

Zhang

et al. 2016

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157

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“…The linear range of the calibration curve obtained with this method was from 3.2 × 10 −8 to 2.4 × 10 −6 mol L −1 and the detection limit is 3.0 × 10 −9 mol L −1 . Yin et al, tagged cyt c binding aptamer with a fluorescent probe called carboxy fluorescein and used vandium sulfide nanosheets to absorb to the probe and quench its fluorescence efficiency (Yin et al, 2015). The proposed sensing system shows gives a linear range of 0.75 nM to 50 μM, and a limit of detection of 0.50 nM.…”

Section: Fluorescent Labelsmentioning

confidence: 99%

“…The results were compared with the electrochemical technique and found that the detection of cyt c by electrochemical transducer was less favorable in comparison with acoustic transducer. Electro-chemiluminescence and fluorescence based cyt c apta sensors has also been reported (vide supra) (Bin et al, 2016;Yin et al, 2015). Stepnova et al, proposed a novel electrochemical aptasensor for detecting cyt c on the base of glassy carbon electrode modified electropolymerized neutral red and decacarboxylated pillar[5]arene (P[5]A-COOH) bearing terminal neutral red and aminated aptamer specific to cyt c (Stepanova et al, 2016).…”

Section: Aptamer Based Cyt C Biosensorsmentioning

confidence: 99%

Recent advances in cytochrome c biosensing technologies

Manickam

Kaushik

Karunakaran³

et al. 2017

Biosensors and Bioelectronics

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This review is an attempt, for the first time, to describe advancements in sensing technology for cytochrome c (cyt c) detection, at point-of-care (POC) application. Cyt c, a heme containing metalloprotein is located in the intermembrane space of mitochondria and released into bloodstream during pathological conditions. The release of cyt c from mitochondria is a key initiative step in the activation of cell death pathways. Circulating cyt c levels represents a novel in-vivo marker of mitochondrial injury after resuscitation from heart failure and chemotherapy. Thus, cyt c detection is not only serving as an apoptosis biomarker, but also is of great importance to understand certain diseases at cellular level. Various existing techniques such as enzyme-linked immunosorbent assays (ELISA), Western blot, high performance liquid chromatography (HPLC), spectrophotometry and flow cytometry have been used to estimate cyt c. However, the implementation of these techniques at POC application is limited due to longer analysis time, expensive instruments and expertise needed for operation. To overcome these challenges, significant efforts are being made to develop electrochemical biosensing technologies for fast, accurate, selective, and sensitive detection of cyt c. Presented review describes the cutting edge technologies available in the laboratories to detect cyt c. The recent advancements in designing and development of electrochemical cyt c biosensors for the quantification of cyt c are also discussed. This review also highlights the POC cyt c biosensors developed recently, that would prove of interest to biologist and therapist to get real time informatics needed to evaluate death process, diseases progression, therapeutics and processes related with mitochondrial injury.

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“…Kong et al (2015) employed prostate specific antigen (PSA) aptamer as the recognition unit for sensitive detection of PSA, and the constructed biosensor was successfully applied in human serum samples. Yin et al (2015) reported a VS 2 nanosheet-based biosensor for detection of cytochrome c.…”

Section: Nucleic Acid-functionalized Transition Metal Nanosheets Fmentioning

confidence: 99%

Nucleic acid-functionalized transition metal nanosheets for biosensing applications

Liu

et al. 2017

Biosensors and Bioelectronics

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In clinical diagnostics, as well as food and environmental safety practices, biosensors are powerful tools for monitoring biological or biochemical processes. Two-dimensional (2D) transition metal nanomaterials, including transition metal chalcogenides (TMCs) and transition metal oxides (TMOs), are receiving growing interest for their use in biosensing applications based on such unique properties as high surface area and fluorescence quenching abilities. Meanwhile, nucleic acid probes based on Watson-Crick base-pairing rules are also being widely applied in biosensing based on their excellent recognition capability. In particular, the emergence of functional nucleic acids in the 1980s, especially aptamers, has substantially extended the recognition capability of nucleic acids to various targets, ranging from small organic molecules and metal ions to proteins and cells. Based on π-π stacking interaction between transition metal nanosheets and nucleic acids, biosensing systems can be easily assembled. Therefore, the combination of 2D transition metal nanomaterials and nucleic acids brings intriguing opportunities in bioanalysis and biomedicine. In this review, we summarize recent advances of nucleic acid-functionalized transition metal nanosheets in biosensing applications. The structure and properties of 2D transition metal nanomaterials are first discussed, emphasizing the interaction between transition metal nanosheets and nucleic acids. Then, the applications of nucleic acid-functionalized transition metal nanosheet-based biosensors are discussed in the context of different signal transducing mechanisms, including optical and electrochemical approaches. Finally, we provide our perspectives on the current challenges and opportunities in this promising field.

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A novel VS₂nanosheet-based biosensor for rapid fluorescence detection of cytochrome c

Abstract: A VS2/aptamer-based cytochromecsensor was successfully constructed by first applying the DNA-adsorbing ability/fluorescence-quenching properties of VS2in bioanalysis.

Cited by 40 publications

References 59 publications

Recent Advances in Synthesis and Biomedical Applications of Two‐Dimensional Transition Metal Dichalcogenide Nanosheets

Recent Advances in Synthesis and Biomedical Applications of Two‐Dimensional Transition Metal Dichalcogenide Nanosheets

Recent advances in cytochrome c biosensing technologies

Nucleic acid-functionalized transition metal nanosheets for biosensing applications

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A novel VS2nanosheet-based biosensor for rapid fluorescence detection of cytochrome c

Abstract: A VS2/aptamer-based cytochromecsensor was successfully constructed by first applying the DNA-adsorbing ability/fluorescence-quenching properties of VS2in bioanalysis.

Cited by 40 publications

References 59 publications

Recent Advances in Synthesis and Biomedical Applications of Two‐Dimensional Transition Metal Dichalcogenide Nanosheets

Recent Advances in Synthesis and Biomedical Applications of Two‐Dimensional Transition Metal Dichalcogenide Nanosheets

Recent advances in cytochrome c biosensing technologies

Nucleic acid-functionalized transition metal nanosheets for biosensing applications

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Resources

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A novel VS₂nanosheet-based biosensor for rapid fluorescence detection of cytochrome c