A Novel Urine-Based Assay for Bladder Cancer Diagnosis: Multi-Institutional Validation Study

Davis, N S; Shtabsky, Alexander; Lew, Sylvia; Rona, Ronny; Leibovitch, Ilan; Nativ, Ofer; Cohen, Michael; Mor, Yoram; Lindner, Uri; Glickman, Yael A.; Matzkin, Haim; Tsivian, Alexander; Gofrit, Ofer N.; Yossepovitch, Ofer

doi:10.1016/j.euf.2016.10.004

Cited by 24 publications

(13 citation statements)

References 23 publications

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“…In addition, the CellDetect assay is a novel histochemical staining platform which allows for the discrimination between normal and malignant cells on the basis of color and morphological discrimination-based on the higher metabolic activity in cancer cells [39]. An Israeli study across 9 hospitals employing urine smears found that the overall sensitivity of this test was 84%, and the specificity was also 84% for patients undergoing routine surveillance by cystoscopy [40]. This test is currently gaining importance by using a cell based assay in clinical practice.…”

Section: Additional Protein Markers Detectable In the Urinementioning

confidence: 99%

Urinary biomarkers in bladder cancer: A review of the current landscape and future directions

Stenzl

Sharma

et al. 2021

Urologic Oncology: Seminars and Original Investigations

138

120

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ActionAdobe Reader version 9 Adobe Reader version X and XI Insert textClick the 'Text Edits' button on the Commenting tool bar. Click to set the cursor location in the text and simply start typing. The text will appear in a commenting box. You may also cut-and-paste text from another file into the commenting box. Close the box by clicking on 'x' in the top right-hand corner.Click the 'Insert Text' icon on the Comment tool bar. Click to set the cursor location in the text and simply start typing. The text will appear in a commenting box. You may also cut-and-paste text from another file into the commenting box. Close the box by clicking on '_' in the top right-hand corner. Replace text Replace textClick the 'Text Edits' button on the Commenting tool bar. To highlight the text to be replaced, click and drag the cursor over the text. Then simply type in the replacement text. The replacement text will appear in a commenting box. You may also cut-and-paste text from another file into this box. To replace formatted text (an equation for example) please Attach a file (see below).Click the 'Replace (Ins)' icon on the Comment tool bar. To highlight the text to be replaced, click and drag the cursor over the text. Then simply type in the replacement text. The replacement text will appear in a commenting box. You may also cut-and-paste text from another file into this box. To replace formatted text (an equation for example) please Attach a file (see below). Remove textClick the 'Text Edits' button on the Commenting tool bar. Click and drag over the text to be deleted. Then press the delete button on your keyboard. The text to be deleted will then be struck through.Click the 'Strikethrough (Del)' icon on the Comment tool bar. Click and drag over the text to be deleted. Then press the delete button on your keyboard. The text to be deleted will then be struck through. Highlight text/ make a commentClick on the 'Highlight' button on the Commenting tool bar. Click and drag over the text. To make a comment, double click on the highlighted text and simply start typing.Click on the 'Highlight Text' icon on the Comment tool bar. Click and drag over the text. To make a comment, double click on the highlighted text and simply start typing. Attach a fileClick on the 'Attach a File' button on the Commenting tool bar. Click on the figure, table or formatted text to be replaced. A window will automatically open allowing you to attach the file. To make a comment, go to 'General' in the 'Properties' window, and then 'Description'. A graphic will appear in the PDF file indicating the insertion of a file.Click on the 'Attach File' icon on the Comment tool bar. Click on the figure, table or formatted text to be replaced. A window will automatically open allowing you to attach the file. A graphic will appear indicating the insertion of a file. Leave a note/ commentClick on the 'Note Tool' button on the Commenting tool bar. Click to set the location of the note on the document and simply start typing. Do not use this feature to make text edits.C...

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Section: Additional Protein Markers Detectable In the Urinementioning

confidence: 99%

Urinary biomarkers in bladder cancer: A review of the current landscape and future directions

Stenzl

Sharma

et al. 2021

Urologic Oncology: Seminars and Original Investigations

138

120

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“…In a multicenter validation study, the test reached a sensitivity of 84% (Table 3) (78% for detecting LG NMIBC). The specificity was 84% in patients undergoing surveillance by cystoscopy [24].…”

Section: Cell-based Bladder Markersmentioning

confidence: 93%

Urinary-Based Markers for Bladder Cancer Detection

Todenhöfer¹,

Lodde²,

Kessel³

et al. 2020

Société Internationale D’Urologie Journal

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Background The use of urine markers for diagnosis and surveillance has been a topic of broad interest and ongoing controversies in the management of patients with bladder cancer. There has been a constant quest for markers that demonstrate clinical utility. Aim In the framework of the International Consultation on Urological Diseases 2019 on Molecular Biomarkers in Urologic Oncology, a comprehensive review of literature on urinary biomarkers for bladder cancer has been performed. Results Currently available urinary markers include protein-based markers, RNA-based markers, and DNA-based markers. The introduction of high-throughput analysis technologies provides the opportunity to assess multiple parameters within a short period of time, which is of interest for RNA-based, DNA-based, and protein-based marker systems. A comprehensive analysis of molecular alterations in urine samples of bladder cancer patients may be of interest not only for diagnosis and surveillance but also for non-invasive longitudinal assessment of molecular, potentially therapy-relevant, alterations. However, most systems lack prospective validation within well-designed trials and have not been broadly implemented in daily clinical practice. Conclusions Because of limited data from prospective trials, the routine use of any urine marker except cytology is not considered as standard of care in international guidelines. There is an urgent need for prospective trials of urine markers to answer specific clinical questions.

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“…CellDetect is a histochemical staining technique that utilizes color and morphology to discriminate between malignant and benign cells based on differences in metabolic signatures. In two studies, CellDetect exhibited a higher sensitivity for low-grade tumors than urine cytology (82% vs. 59%) and a similar specificity (86% vs. 94%) [ 57 , 58 ].…”

Section: Urine Protein Biomarkersmentioning

confidence: 99%

Proteomics for Early Detection of Non-Muscle-Invasive Bladder Cancer: Clinically Useful Urine Protein Biomarkers

Ahn

Kang

Kim

et al. 2022

Life

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Bladder cancer is the fourth most common cancer in men, and most cases are non-muscle-invasive. A high recurrence rate is a critical problem in non-muscle-invasive bladder cancer. The availability of few urine tests hinders the effective detection of superficial and small bladder tumors. Cystoscopy is the gold standard for diagnosis; however, it is associated with urinary tract infections, hematuria, and pain. Early detection is imperative, as intervention influences recurrence. Therefore, urinary biomarkers need to be developed to detect these bladder cancers. Recently, several protein candidates in the urine have been identified as biomarkers. In the present narrative review, the current status of the development of urinary protein biomarkers, including FDA-approved biomarkers, is summarized. Additionally, contemporary proteomic technologies, such as antibody-based methods, mass-spectrometry-based methods, and machine-learning-based diagnosis, are reported. Furthermore, new strategies for the rapid and correct profiling of potential biomarkers of bladder cancer in urine are introduced, along with their limitations. The advantages of urinary protein biomarkers and the development of several related technologies are highlighted in this review. Moreover, an in-depth understanding of the scientific background and available protocols in research and clinical applications of the surveillance of non-muscle bladder cancer is provided.

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A Novel Urine-Based Assay for Bladder Cancer Diagnosis: Multi-Institutional Validation Study

Cited by 24 publications

References 23 publications

Urinary biomarkers in bladder cancer: A review of the current landscape and future directions

Urinary biomarkers in bladder cancer: A review of the current landscape and future directions

Urinary-Based Markers for Bladder Cancer Detection

Proteomics for Early Detection of Non-Muscle-Invasive Bladder Cancer: Clinically Useful Urine Protein Biomarkers

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