A novel urine analysis technique combining affinity chromatography with Au nanoparticle based surface enhanced Raman spectroscopy for potential applications in non‐invasive cancer screening

Lin, Xueliang; Wang, Lingna; Lin, Huijing; Lin, Duo; Lin, Jinyong; Liu, Xiujie; Qiu, Sufang; Xu, Yujin; Chen, Guannan; Feng, Shouhua

doi:10.1002/jbio.201800327

Cited by 25 publications

(19 citation statements)

References 43 publications

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“…Analysing urine through SERS, Moisoiu and colleagues gathered 53 female BC patients undergoing mastectomy/lumpectomy and 22 healthy subjects, having attained 95% specificity, 81% sensitivity and 88% overall accuracy [ 97 ]. Lin et al combined affinity chromatography with SERS for the analysis of urine in order to distinguish BC from other cancer types (namely gastric cancer): breast cancer was differentiated from gastric cancer with 82% sensitivity and 90.7% specificity, while breast cancer was distinguished from healthy individuals with 76.5% sensitivity and 87.5% specificity [ 98 ].…”

Section: Resultsmentioning

confidence: 99%

A New Look into Cancer—A Review on the Contribution of Vibrational Spectroscopy on Early Diagnosis and Surgery Guidance

Mamede

Santos

Carvalho

et al. 2021

Cancers

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In 2020, approximately 10 million people died of cancer, rendering this disease the second leading cause of death worldwide. Detecting cancer in its early stages is paramount for patients’ prognosis and survival. Hence, the scientific and medical communities are engaged in improving both therapeutic strategies and diagnostic methodologies, beyond prevention. Optical vibrational spectroscopy has been shown to be an ideal diagnostic method for early cancer diagnosis and surgical margins assessment, as a complement to histopathological analysis. Being highly sensitive, non-invasive and capable of real-time molecular imaging, Raman and Fourier transform infrared (FTIR) spectroscopies give information on the biochemical profile of the tissue under analysis, detecting the metabolic differences between healthy and cancerous portions of the same sample. This constitutes tremendous progress in the field, since the cancer-prompted morphological alterations often occur after the biochemical imbalances in the oncogenic process. Therefore, the early cancer-associated metabolic changes are unnoticed by the histopathologist. Additionally, Raman and FTIR spectroscopies significantly reduce the subjectivity linked to cancer diagnosis. This review focuses on breast and head and neck cancers, their clinical needs and the progress made to date using vibrational spectroscopy as a diagnostic technique prior to surgical intervention and intraoperative margin assessment.

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Section: Resultsmentioning

confidence: 99%

A New Look into Cancer—A Review on the Contribution of Vibrational Spectroscopy on Early Diagnosis and Surgery Guidance

Mamede

Santos

Carvalho

et al. 2021

Cancers

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“…Pesticide thiabendazole (TBZ) is globally used as a food fungicide in agriculture to endow citrus fruits with freshness. − However, the abuse of TBZ and its excess residue in food severely threaten human health, which has received immense attention and is needed to be addressed urgently. Surface-enhanced Raman scattering (SERS) with a promising advantage of nondestructive examination has been confirmed as a powerful and versatile protocol in food safety, biological detection, chemical analysis, and pollutant monitoring. − It is generally accepted that SERS provides significant amplification of Raman signals for analytes near the plasmonic nanostructures based on gold (Au) and/or silver (Ag) nanoparticles (NPs) regarding the localized surface plasmonic resonance (LSPR) effect. − In recent decades, many efforts have been made to develop sophisticated SERS-active substrates for the sensitive analysis of food contaminants. As it is reported, some conventional rigid SERS substrates have exhibited optimized reproducibility and homogeneity. , However, without flexibility, the application of these rigid substrates for in situ monitoring analytes on curved objects has been limited .…”

Section: Introductionmentioning

confidence: 99%

Contrastive Study of In Situ Sensing and Swabbing Detection Based on SERS-Active Gold Nanobush–PDMS Hybrid Film

Chen

Tian

et al. 2021

J. Agric. Food Chem.

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Surface-enhanced Raman scattering (SERS) with fast and intuitive property has been extensively utilized in the field of food safety. Here, we demonstrated a novel noble metal–polymer hybrid film as a SERS substrate for food fungicide analysis. Benefiting from its transparency and flexibility, poly(dimethylsiloxane) (PDMS) film was chosen as a versatile supporting matrix to grow gold nanobushes (Au NBs) through a seed-mediated process. The as-prepared AuNB–PDMS hybrid film performed satisfactorily in testing 4-nitrothiophenol (4NTP) and exhibited an enhancement factor (EF) of 2.56 × 106. Moreover, the high sensitivity and elastic properties make the hybrid film a promising substrate in practical detection. Hence, the in situ sensing of TBZ, carbaryl, and their mixture was finally realized using the developed hybrid film, which exhibited higher sensitivity than that obtained by the swabbing method. This high-performance SERS substrate based on the flexible and transparent AuNB–PDMS hybrid film has great potential applications in the fast in situ monitoring of biochemical molecules.

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“…Cancer causes complex changes in the human body, which are difficult to detect with individual biomarkers especially at early stages of the disease. One important advantage of SERS is that it facilitates the detection of cancer‐associated changes in the molecular “fingerprint” of biological fluids, such as saliva , urine and blood. SERS‐based in vitro diagnosis of whole blood , serum and plasma were extensively explored.…”

Section: Introductionmentioning

confidence: 99%

Label‐free diagnosis for colorectal cancer through coffee ring‐assisted surface‐enhanced Raman spectroscopy on blood serum

Yan

Huang

et al. 2020

Journal of Biophotonics

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Surface-enhanced Raman spectroscopy (SERS) is garnering considerable attention for the swift diagnosis of pathogens and abnormal biological status, that is, cancers. In this work, a simple, fast and inexpensive optical sensing platform is developed by the design of SERS sampling and data analysis. The pretreatment of spectral measurement employed gold nanoparticle colloid mixing with the serum from patients with colorectal cancer (CRC). The droplet of particle-serum mixture formed coffee-ring-like region at the rim, providing strong and stable SERS profiles. The obtained spectra from cancer patients and healthy volunteers were analyzed by unsupervised principal component analysis (PCA) and supervised machine learning model, such as support-vector machine (SVM), respectively. The results demonstrate that the SVM model provides the superior performance in the classification of CRC diagnosis compared with PCA. In addition, the values of carcinoembryonic antigen from the blood samples were compiled with the corresponding SERS spectra for SVM calculation, yielding improved prediction results. K E Y W O R D S colorectal cancer diagnosis, label-free detection, machine learning, nanoparticles, SERS

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A novel urine analysis technique combining affinity chromatography with Au nanoparticle based surface enhanced Raman spectroscopy for potential applications in non‐invasive cancer screening

Cited by 25 publications

References 43 publications

A New Look into Cancer—A Review on the Contribution of Vibrational Spectroscopy on Early Diagnosis and Surgery Guidance

A New Look into Cancer—A Review on the Contribution of Vibrational Spectroscopy on Early Diagnosis and Surgery Guidance

Contrastive Study of In Situ Sensing and Swabbing Detection Based on SERS-Active Gold Nanobush–PDMS Hybrid Film

Label‐free diagnosis for colorectal cancer through coffee ring‐assisted surface‐enhanced Raman spectroscopy on blood serum

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