A novel tripolymer coating demonstrating the synergistic effect of chitosan, collagen type 1 and hyaluronic acid on osteogenic differentiation of human bone marrow derived mesenchymal stem cells

Mathews, Smitha; Bhonde, Ramesh; Gupta, Pawan Kumar; Totey, S.M.

doi:10.1016/j.bbrc.2011.09.071

Cited by 56 publications

(46 citation statements)

References 31 publications

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“…We suppose that this latter phenomenon was at the basis of the amplification of differentiation that we observed when the cells were cultivated in the presence of electrospun collagen and osteogenic supplementations Biomimetic approaches mimicking ECM has been variously attempted using both synthetic and natural polymers and combination of them. Collagen alone 39 or in combination with other components of ECM such as hyaluronic acid 40 or other polymers such as chitosans 41 or fibroins 42 or have been demonstrated to induce in vitro osteogenic differentiation of MSCs. Fig.…”

Section: Resultsmentioning

confidence: 99%

Electrospun Collagen Mimicking the Reconstituted Extracellular Matrix Improves Osteoblastic Differentiation Onto Titanium Surfaces

Iafisco¹,

Quirici²,

Foltran³

et al. 2013

j. nanosci. nanotech.

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Titanium and its alloys are the current materials to manufacture oral implants because of their excellent mechanical properties and biocompatibility. However the increasing needs of the patients to receive fast and reliable rehabilitation have forced materials scientists to modified the surface of the materials in order to increase the rate of osseointegration and minimize the times for healing. The presence of a reconstituted extracellular matrix (ECM), constituted of proteins and polysaccharides is a key factor for healing and regeneration of the tissues. The nano-fibrous feature of ECM improves cells proliferation and addresses their phenotype. Electrospinning technique is an efficient processing method to manufacture micro- and nano-sized fibrous structures mimicking the ECM. In this work we describe a method to obtain collagen coating made of nano-fibers onto titanium for oral implant manufacturing, using electrospinning. The obtained collagen coatings showed morphology, structure and chemical composition similar to that of ECM. Moreover the stem cells cultured onto titanium samples coated with electrospun collagen showed faster osteoblastic differentiation and more efficient deposition of mineralized matrix in comparison with those onto uncoated substrates. This effect was amplified using osteogenetic media.

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Section: Resultsmentioning

confidence: 99%

Electrospun Collagen Mimicking the Reconstituted Extracellular Matrix Improves Osteoblastic Differentiation Onto Titanium Surfaces

Iafisco¹,

Quirici²,

Foltran³

et al. 2013

j. nanosci. nanotech.

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“…Chitosan scaffold has been found to be biodegradable and biocompatible with excellent structural stability [10]. It has been used for MSC transfer in the past [12,13]. In order to differentiate between the positive spacer effects of chitosan versus the regeneration potential of MSC, we used chitosan alone in some and chitin carrying allogenic stem cells in some other animals and found that chitin was not a good spacer, as the angulation of the distal femurs in chitin group was similar to the contralateral control side.…”

Section: Discussionmentioning

confidence: 99%

“…Natural cationic biopolymer chitosan is a partially deacetylated derivative of chitin. Chitosan is described as possessing biological and material properties suitable for biomedical and clinical applications [12,13]. It has a good mechanical stability and is biocompatible, promotes hyaluran synthesis and is also shown to enhance wound healing.…”

Section: Groupmentioning

confidence: 99%

Tissue engineering strategy using mesenchymal stem cell-based chitosan scafolds in growth plate surgery: A preliminary study in rabbits

Azarpira

Shahcheraghi

Ayatollahi

et al. 2015

Orthopaedics & Traumatology: Surgery & Research

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“…Large proteins or glycosaminoclycans such as collagen and chondroitin sulfate provide a biomimetic coating on the surface of an implant which can improve integration once implanted in the body [40, 41]. Growth factors are another type of widely used biomolecules for implant coatings due to their ability to modulate cellular functions such as decreasing inflammation, enhancing stem cell differentiation, inducing blood vessel formation, or acting as chemoattractants for circulating osteoprogenitors [42-44].…”

Section: Coatings To Enhance Osseointegrationmentioning

confidence: 99%

The future of biologic coatings for orthopaedic implants

Goodman¹,

Yao²,

Keeney³

et al. 2013

Biomaterials

699

507

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Implants are widely used for othopaedic applications such as fixing fractures, repairing nonunions, obtaining a joint arthrodesis, total joint arthroplasty, spinal reconstruction, and soft tissue anchorage. Previously, orthopaedic implants were designed simply as mechanical devices; the biological aspects of the implant were a byproduct of stable internal/external fixation of the device to the surrounding bone or soft tissue. More recently, biologic coatings have been incorporated into orthopaedic implants in order to modulate the surrounding biological environment. This opinion article reviews current and potential future use of biologic coatings for orthopaedic implants to facilitate osseointegration and mitigate possible adverse tissue responses including the foreign body reaction and implant infection. While many of these coatings are still in the preclinical testing stage, bioengineers, material scientists and surgeons continue to explore surface coatings as a means of improving clinical outcome of patients undergoing orthopaedic surgery.

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A novel tripolymer coating demonstrating the synergistic effect of chitosan, collagen type 1 and hyaluronic acid on osteogenic differentiation of human bone marrow derived mesenchymal stem cells

Cited by 56 publications

References 31 publications

Electrospun Collagen Mimicking the Reconstituted Extracellular Matrix Improves Osteoblastic Differentiation Onto Titanium Surfaces

Electrospun Collagen Mimicking the Reconstituted Extracellular Matrix Improves Osteoblastic Differentiation Onto Titanium Surfaces

Tissue engineering strategy using mesenchymal stem cell-based chitosan scafolds in growth plate surgery: A preliminary study in rabbits

The future of biologic coatings for orthopaedic implants

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