Tissue engineering strategy using mesenchymal stem cell-based chitosan scafolds in growth plate surgery: A preliminary study in rabbits

Azarpira, Mohammad Reza; Shahcheraghi, Gholam Hossain; Ayatollahi, Maryam; Geramizadeh, Bita

doi:10.1016/j.otsr.2015.04.010

Cited by 17 publications

(16 citation statements)

References 24 publications

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“…Azarpira et al . reported that growth arrest model in which the lateral 50% of the distal femoral physis was removed and was filled with a MSC-based chitosan scaffold with a resulting trend toward less angular deformity25. The aspects of autogenous vs. allogeneic MSC transplantation into the injured physeal growth zone of extremity bones remain a problem.…”

Section: Discussionmentioning

confidence: 99%

Treatment of rabbit growth plate injuries with oriented ECM scaffold and autologous BMSCs

Huang

et al. 2017

Sci Rep

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Tissue-engineered technology has provided a promising method for the repair of growth plate injuries using biocompatible and biodegradable scaffolds and appropriate cells. The aim of this study was to fabricate oriented ECM scaffolds to imitate the material and structure of a natural growth plate and to investigate whether BMSCs in a scaffold could prevent the formation of bone bridges in an injured growth plate. We developed a natural, acellular and oriented scaffold derived from a growth plate. The oriented scaffold was fabricated using new freeze-drying technology and by cross-linking the microfilaments in the growth plate. From histological examination, the scaffold contained most of the ECM components including GAG and collagen II without cell DNA fragments, and SEM revealed that oriented scaffold had a uniform aperture in the transverse plane and columnar structure in length plane. Cytotoxicity testing with MTT showed no cytotoxic effect of the scaffold extracts on BMSCs. Autogenous BMSCs in oriented scaffolds promoted the regeneration of neogenetic growth plate when repairing an injured growth plate and prevent the formation of bone bridges to reduce the angular deformity and length discrepancy in the proximal tibia in rabbits. The well-characterized ECM-derived oriented growth plate scaffold shows potential for the repair of injured growth plates in young rabbits.

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Section: Discussionmentioning

confidence: 99%

Treatment of rabbit growth plate injuries with oriented ECM scaffold and autologous BMSCs

Huang

et al. 2017

Sci Rep

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“…Regenerative cell therapies in the fast-emerging field of tissue engineering are based on the anticipation that delivered cells rebuild the affected tissues [ 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 ]. Various technologies tested to introduce adequate stem cells for the construction of functioning tissues and organs, both in vitro and in vivo, have had only minimal success [ 8 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 ]. In most cases, in vitro-constructed non-vascularized 3D tissue-engineered complex tissues based on differentiated stem cells seemed to fade upon their implantation.…”

Section: Development Of the Concept Of Cell Therapy For Pro-regenerative Treatmentsmentioning

confidence: 99%

Allogenic Use of Human Placenta-Derived Stromal Cells as a Highly Active Subtype of Mesenchymal Stromal Cells for Cell-Based Therapies

Gorodetsky

Aicher

2021

IJMS

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The application of mesenchymal stromal cells (MSCs) from different sources, including bone marrow (BM, bmMSCs), adipose tissue (atMSCs), and human term placenta (hPSCs) has been proposed for various clinical purposes. Accumulated evidence suggests that the activity of the different MSCs is indirect and associated with paracrine release of pro-regenerative and anti-inflammatory factors. A major limitation of bmMSCs-based treatment for autologous application is the limited yield of cells harvested from BM and the invasiveness of the procedure. Similar effects of autologous and allogeneic MSCs isolated from various other tissues were reported. The easily available fresh human placenta seems to represent a preferred source for harvesting abundant numbers of human hPSCs for allogenic use. Cells derived from the neonate tissues of the placenta (f-hPSC) can undergo extended expansion with a low risk of senescence. The low expression of HLA class I and II on f-hPSCs reduces the risk of rejection in allogeneic or xenogeneic applications in normal immunocompetent hosts. The main advantage of hPSCs-based therapies seems to lie in the secretion of a wide range of pro-regenerative and anti-inflammatory factors. This renders hPSCs as a very competent cell for therapy in humans or animal models. This review summarizes the therapeutic potential of allogeneic applications of f-hPSCs, with reference to their indirect pro-regenerative and anti-inflammatory effects and discusses clinical feasibility studies.

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“…encapsulation, tissue scaffolding or as a material for degradable regeneration structures [50][51][52][53]. For these composite and polymer applications, the following requirements are posed: no toxicity for the human body, no harmful interaction with the drug, no metabolic polymer transformation products excreted beyond the body, and beneficial mechanical properties if possible, to allow the transfer of the resulting stresses to the implant before the tissue recovers [50,54,55]. In the second case, composites find a structural application, i.e.…”

Section: Polymer-ceramic Compositesmentioning

confidence: 99%

Cenospheres and their application advantages in biomedical engineering - a systematic review

Nakonieczny

Antonowicz

Paszenda

2020

Reviews on Advanced Materials Science

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Aluminum-silicate cenospheres are the most valuable residue present in fly ashes after combusting stone coal. Cenospheres are hollow bodies with desirable engineering properties, such as hardness, low bulk density and complete chemical inertness, thanks to which they can be used in biomedical engineering. The following review presents data on obtaining and processing the material, as well as potential biomedical applications.

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Tissue engineering strategy using mesenchymal stem cell-based chitosan scafolds in growth plate surgery: A preliminary study in rabbits

Abstract: Level III. Low power comparative study.

Cited by 17 publications

References 24 publications

Treatment of rabbit growth plate injuries with oriented ECM scaffold and autologous BMSCs

Treatment of rabbit growth plate injuries with oriented ECM scaffold and autologous BMSCs

Allogenic Use of Human Placenta-Derived Stromal Cells as a Highly Active Subtype of Mesenchymal Stromal Cells for Cell-Based Therapies

Cenospheres and their application advantages in biomedical engineering - a systematic review

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