A novel transcriptional regulator RXE1 modulates the essential transactivator XYR1 and cellulase gene expression in Trichoderma reesei

Wang, Lei; Lv, Xinxing; Cao, Yanli; Zheng, Fanglin; Meng, Xiangfeng; Shen, Yu; Chen, Guanjun; Liu, Weifeng; Zhang, Weixin

doi:10.1007/s00253-019-09739-6

Cited by 39 publications

(37 citation statements)

References 46 publications

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“…Trichoderma reesei QM9414 (ATCC 26921) and QM9414Δ pyr4 in which the uridine trophic marker gene was deleted in QM9414 (Wang et al , ) were used throughout this work as control and parental strains respectively. All T. reesei strains were maintained on malt extract agar supplemented with 10 mM of uridine when necessary.…”

Section: Methodsmentioning

confidence: 99%

Trichoderma reesei XYR1 recruits SWI/SNF to facilitate cellulase gene expression

Cao

Zheng

Zhang

et al. 2019

Molecular Microbiology

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Summary Cellulase gene expression in Trichoderma reesei is highly responsive to environmental cues and is under stringent regulation by multiple transcription factors. XYR1 (Xylanase regulator 1) has been identified as the most important transcriptional activator of cellulase/hemicellulase gene expression although the precise transactivating mechanism remains largely elusive. Here we show that the activation domain of XYR1 interacts with the T. reesei homolog of the TrSNF12 subunit of SWI/SNF complex. Deletion of Trsnf12 markedly impaired the induced cellulase gene expression. Individual loss of other SWI/SNF subunits including the catalytic subunit also severely compromised cellulase gene expression and interfered with loss of histone H4 in the cbh1 and eg1 promoters upon cellulose induction. In addition, we find that the SWI/SNF occupancy on cellulase gene promoters strictly required XYR1 and TrSNF12 but TrSNF12 was dispensable for the XYR1 binding to these promoters. These data suggest a model in which XYR1 recruits SWI/SNF through direct interactions with TrSNF12 to remodel chromatin at cellulase gene promoters, thereby activating cellulase gene expression to initiate the cellulolytic response in T. reesei.

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Section: Methodsmentioning

confidence: 99%

Trichoderma reesei XYR1 recruits SWI/SNF to facilitate cellulase gene expression

Cao

Zheng

Zhang

et al. 2019

Molecular Microbiology

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“…In P. decumbens, an industrial lignocellulolytic enzymes production strain, expression of cellulases genes is upregulated in BrlA deletion strain [81]. In T. reesei, while no ortholog has been identified for BrlA, rxe1 (20 % identity with BrlA) is involved in regulation of conidiation and modulated positively by the expression of xyr1 and cellulase and hemicellulase genes [32]. The regulatory cascade between aba1 and wet-1 is preserved in N. crassa and F. graminearum but we do not know if the rxe1 gene could replace BrlA in species where there is no true ortholog and therefore if wet-1 may be controlled by rxe1 .…”

Section: Discussionmentioning

confidence: 99%

Glucose-lactose mixture feeds in industry-like conditions: a gene regulatory network analysis on the hyperproducing Trichoderma reesei strain Rut-C30

Pirayre

Duval

Blugeon

et al. 2020

Preprint

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Background: The degradation of cellulose and hemicellulose molecules into simpler sugars such as glucose is part of the second generation biofuel production process. Hydrolysis of lignocellulosic substrates is usually performed by enzymes produced and secreted by the fungus Trichoderma reesei . Studies identifying transcription factors involved in the regulation of cellulase production have been conducted but no overview of the whole regulation network is available. A transcriptomic approach with mixtures of glucose and lactose, used as a substrate for cellulase induction, was used to help us decipher missing parts in the network of T. reesei Rut-C30.Results: Experimental results on the Rut-C30 hyperproducing strain confirmed the impact of sugar mixtures on the enzymatic cocktail composition. The transcriptomic study shows a temporal regulation of the main transcription factors and a lactose concentration impact on the transcriptional profile. A gene regulatory network built using BRANE Cut software reveals three sub-networks related to iq a positive correlation between lactose concentration and cellulase production, iiq a particular dependence of the lactose onto the β-glucosidase regulation and iiiq a negative regulation of the development process and growth.Conclusions: This work is the first investigating a transcriptomic study regarding the effects of pure and mixed carbon sources in a fed-batch mode. Our study expose a co-orchestration of xyr1 , clr2 and ace3 for cellulase and hemicellulase induction and production, a fine regulation of the β-glucosidase and a decrease of growth in favor of cellulase production. These conclusions provide us with potential targets for further genetic engineering leading to bettercellulase-producing strains in industry-like conditions.

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“…T. reesei QM9414 (ATCC 26921) and QM9414Δpyr4 in which the uridine trophic marker gene was deleted in QM9414 [52] were used throughout this work as control and parental strains, respectively. All T. reesei strains were maintained on malt extract agar supplemented with 10 mM uridine when necessary.…”

Section: Strains and Cultivation Conditionmentioning

confidence: 99%

Trichoderma reesei XYR1 activates cellulase gene expression via interaction with the Mediator subunit TrGAL11 to recruit RNA polymerase II

et al. 2020

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The ascomycete Trichoderma reesei is a highly prolific cellulase producer. While XYR1 (Xylanase regulator 1) has been firmly established to be the master activator of cellulase gene expression in T. reesei, its precise transcriptional activation mechanism remains poorly understood. In the present study, TrGAL11, a component of the Mediator tail module, was identified as a putative interacting partner of XYR1. Deletion of Trgal11 markedly impaired the induced expression of most (hemi)cellulase genes, but not that of the major β-glucosidase encoding genes. This differential involvement of TrGAL11 in the full induction of cellulase genes was reflected by the RNA polymerase II (Pol II) recruitment on their core promoters, indicating that TrGAL11 was required for the efficient transcriptional initiation of the majority of cellulase genes. In addition, we found that TrGAL11 recruitment to cellulase gene promoters largely occurred in an XYR1-dependent manner. Although xyr1 expression was significantly tuned down without TrGAL11, the binding of XYR1 to cellulase gene promoters did not entail TrGAL11. These results indicate that TrGAL11 represents a direct in vivo target of XYR1 and may play a critical role in contributing to Mediator and the following RNA Pol II recruitment to ensure the induced cellulase gene expression.

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A novel transcriptional regulator RXE1 modulates the essential transactivator XYR1 and cellulase gene expression in Trichoderma reesei

Cited by 39 publications

References 46 publications

Trichoderma reesei XYR1 recruits SWI/SNF to facilitate cellulase gene expression

Trichoderma reesei XYR1 recruits SWI/SNF to facilitate cellulase gene expression

Glucose-lactose mixture feeds in industry-like conditions: a gene regulatory network analysis on the hyperproducing Trichoderma reesei strain Rut-C30

Trichoderma reesei XYR1 activates cellulase gene expression via interaction with the Mediator subunit TrGAL11 to recruit RNA polymerase II

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A novel transcriptional regulator RXE1 modulates the essential transactivator XYR1 and cellulase gene expression in Trichoderma reesei

Cited by 39 publications

References 46 publications

Trichoderma reesei XYR1 recruits SWI/SNF to facilitate cellulase gene expression

Trichoderma reesei XYR1 recruits SWI/SNF to facilitate cellulase gene expression

Glucose-lactose mixture feeds in industry-like conditions: a gene regulatory network analysis on the hyperproducing Trichoderma reesei strain&nbsp;Rut-C30

Trichoderma reesei XYR1 activates cellulase gene expression via interaction with the Mediator subunit TrGAL11 to recruit RNA polymerase II

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Glucose-lactose mixture feeds in industry-like conditions: a gene regulatory network analysis on the hyperproducing Trichoderma reesei strain Rut-C30