A Novel Thiazolyl Schiff Base: Antibacterial and Antifungal Effects and In Vitro Oxidative Stress Modulation on Human Endothelial Cells

Abstract

Schiff bases (SBs) are chemical compounds displaying a significant pharmacological potential. They are able to modulate the activity of many enzymes involved in metabolism and are found among antibacterial, antifungal, anti-inflammatory, antioxidant, and antiproliferative drugs. A new thiazolyl-triazole SB was obtained and characterized by elemental and spectral analysis. The antibacterial and antifungal ability of the SB was evaluated against Gram-positive and Gram-negative bacteria and against three Candida strains. SB showed good antibacterial activity against L. monocytogenes and P. aeruginosa; it was two times more active than ciprofloxacin. Anti-Candida activity was twofold higher compared with that of fluconazole. The effect of the SB on cell viability was evaluated by colorimetric measurement on cell cultures exposed to various SB concentrations. The ability of the SB to modulate oxidative stress was assessed by measuring MDA, TNF-α, SOD1, COX2, and NOS2 levels in vitro, using human endothelial cell cultures exposed to a glucose-enriched medium. SB did not change the morphology of the cells. Experimental findings indicate that the newly synthetized Schiff base has antibacterial activity, especially on the Gram-negative P. aeruginosa, and antifungal activity. SB also showed antioxidant and anti-inflammatory activities.

Oxidative Stress and Free Radical Processes in Tumor and Non-Tumor Obstructive Jaundice: Influence of Disease Duration, Severity and Surgical Treatment on Outcomes

“…These protein-lipid complexes often form strong chemical bonds between the free amino groups of amino acid radicals and the aldehyde (or carboxyl) groups of oxidized lipids. The most significant role in the formation of these complexes is played by MDA, which as one of the end-products of the lipid peroxidation process, forms Schiff bases and reacts with lysine ε-amino groups or residues of N-terminal amino acids in polypeptide molecules [ 36 , 37 , 38 ]. Therefore, measuring MDA can be recommended as an additional diagnostic test for assessing the severity of and prognosis for jaundice.…”

Substitution reactivity and structural variability induced by tryptamine on the biomimetic rhenium tricarbonyl complex

“…Schiff bases have been of significant interest to chemists due to their ease of synthesis and the variable properties achieved in the final product. 1 From a clinical perspective they boast a broad range of medicinal application such as antiviral, 2 antibacterial 3 and antimalarial 4 characteristics. These characteristics are dependent on the substitutes bound to the imine functionality, while the final properties of the Schiff base can be tuned to fit a particular need.…”

Chemiluminescence Detection in the Study of Free-Radical Reactions. Part 1

“…The most common marker of free radical reactions and the state of oxidative stress is one of the products of lipid peroxidation, malondialdehyde (MDA), whose concentration is determined using thiobarbituric acid (TBA) [ 65 , 66 ]. In order to obtain more reliable results, the concentration of Schiff bases [ 67 , 68 ], diene [ 69 , 70 ], and triene [ 67 ] conjugates should be also measured. Other methods are based on the use of radical scavengers: antioxidant enzymes such as catalase (H 2 O 2 ) [ 71 ] and superoxide dismutase (O 2 ●− ) [ 69 ], phenolic antioxidants for hydroxyl/lipid radicals, and other organic molecules [ 71 ].…”