A novel thermostable chitinase (PJC) from pomegranate (Punica granatum) juice

Kopparapu, Narasimha Kumar; Liu, Zhuqing; Yan, Qiaojuan; Jiang, Zhengqiang; Zhang, Shuping

doi:10.1016/j.foodchem.2011.02.020

Cited by 27 publications

(19 citation statements)

References 37 publications

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“…However, a pomegranate chitinase did not exhibit any antifungal activity (Kopparapu et al, 2011). The thermal stability determination to antifungal activities demonstrated that the chitinase displayed a relative thermostability in antifungal activity.…”

Section: Antifungal Activitymentioning

confidence: 91%

“…A relatively heat-resistant enzyme from fava seeds would certainly be significant in its potential application in agriculture. However, a chitinase from pomegranate shows more higher thermostability (Kopparapu, Liu, Yan, Jiang, & Zhang, 2011), it has optimal activity at pH 4.5 and 70°C, and the literature is lacking in the structure information.…”

Section: Thermostabilitymentioning

confidence: 99%

See 1 more Smart Citation

A novel chitinase isolated from Vicia faba and its antifungal activity

Wang

Chen

et al. 2012

Food Research International

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Section: Antifungal Activitymentioning

confidence: 91%

Section: Thermostabilitymentioning

confidence: 99%

A novel chitinase isolated from Vicia faba and its antifungal activity

Wang

Chen

et al. 2012

Food Research International

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“…The hyphal extension-inhibition assay was evaluated by using the previously described method with some modification (Kopparapu et al 2011). The fresh grown fungal culture (5 days) was inoculated into the center of the petri dishes containing 10 mL PDA.…”

Section: Antifungal Activity Assaymentioning

confidence: 99%

Kaempferol loaded lecithin/chitosan nanoparticles: preparation, characterization, and their potential applications as a sustainable antifungal agent

Torun

Saglam

Özgen

2016

Artificial Cells, Nanomedicine, and Biotechnology

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Flavonoid compounds are strong antioxidant and antifungal agents but their applications are limited due to their poor dissolution and bioavailability. The use of nanotechnology in agriculture has received increasing attention, with the development of new formulations containing active compounds. In this study, kaempferol (KAE) was loaded into lecithin/chitosan nanoparticles (LC NPs) to determine antifungal activity compared to pure KAE against the phytopathogenic fungus Fusarium oxysporium to resolve the bioavailability problem. The influence of formulation parameters on the physicochemical properties of KAE loaded lecithin chitosan nanoparticles (KAE-LC NPs) were studied by using the electrostatic self-assembly technique. KAE-LC NPs were characterized in terms of physicochemical properties. KAE has been successfully encapsulated in LC NPs with an efficiency of 93.8 ± 4.28% and KAE-LC NPs showed good physicochemical stability. Moreover, in vitro evaluation of the KAE-LC NP system was made by the release kinetics, antioxidant and antifungal activity in a time-dependent manner against free KAE. Encapsulated KAE exhibited a significantly inhibition efficacy (67%) against Fusarium oxysporium at the end of the 60 day storage period. The results indicated that KAE-LC NP formulation could solve the problems related to the solubility and loss of KAE during use and storage. The new nanoparticle system enables the use of smaller quantities of fungicide and therefore, offers a more environmentally friendly method of controlling fungal pathogens in agriculture.

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“…Thermophilic fungal strains are also capable of rapid growth at elevated growth temperatures, thereby enhancing the productivity in fermentors . Although chitinases have been reported from a few thermophilic fungi, the potential of the thermostable chitinases in efficient chitin hydrolysis remains to be explored …”

Section: Introductionmentioning

confidence: 99%

Recombinant exochitinase of the thermophilic mould Myceliopthora thermophila BJA: Characteristics and utility in generating N‐acetyl glucosamine and in biocontrol of phytopathogenic fungi

Dua

Joshi

Satyanarayana

2016

Biotechnology Progress

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Chitinase from the thermophilic mould Myceliopthora thermophila BJA (MtChit) is an acid tolerant, thermostable and organic solvent stable biocatalyst which does not require any metal ions for its activity. To produce high enzyme titres, reduce fermentation time and overcome the need for induction, this enzyme has been heterologously expressed under GAP promoter in the GRAS yeast, Pichia pastoris. The production medium supplemented with the permeabilizing agent Tween-20 supported two-fold higher rMtChit production (5.5 × 10 U L ). The consensus sequences S(132)xG(133)G(134) and D(168)xxD(171)xD(173)xE(175) in the enzyme have been found to represent the substrate binding and catalytic sites, respectively. The rMtChit, purified to homogeneity by a two-step purification strategy, is a monomeric glycoprotein of ∼48 kDa, which is optimally active at 55°C and pH 5.0. The enzyme is thermostable with t values of 113 and 48 min at 65 and 75°C, respectively. Kinetic parameters K , V , k , and k /K of the enzyme are 4.655 mg mL , 34.246 nmol mg s , 3.425 × 10 min , and 1.36 × 10 mg mL min , respectively. rMtChit is an unique exochitinase, since its action on chitin liberates N-acetylglucosamine NAG. The enzyme inhibits the growth of phytopathogenic fungi like Fusarium oxysporum and Curvularia lunata, therefore, this finds application as biofungicide at high temperatures during summer in tropics. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 33:70-80, 2017.

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A novel thermostable chitinase (PJC) from pomegranate (Punica granatum) juice

Cited by 27 publications

References 37 publications

A novel chitinase isolated from Vicia faba and its antifungal activity

A novel chitinase isolated from Vicia faba and its antifungal activity

Kaempferol loaded lecithin/chitosan nanoparticles: preparation, characterization, and their potential applications as a sustainable antifungal agent

Recombinant exochitinase of the thermophilic mould Myceliopthora thermophila BJA: Characteristics and utility in generating N‐acetyl glucosamine and in biocontrol of phytopathogenic fungi

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