2009
DOI: 10.1111/j.1600-0854.2009.00879.x
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A Novel Strategy for the Invasive Toxin: Hijacking Exosome‐Mediated Intercellular Trafficking

Abstract: †These authors contributed equally to this work.Toxins penetrate mammalian cells through various means. In this study, we report a unique strategy used by trichosanthin (TCS), a plant toxin with ribosome-inactivating activity, to penetrate host cells. We found that in both JAR and K562 cells, endocytosed TCS is incorporated into intraluminal vesicles of the multivesicular body (MVB) and is then secreted in association with these vesicles upon fusion of the MVB with the plasma membrane. The secreted TCS-loaded … Show more

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Cited by 27 publications
(14 citation statements)
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“…Second, the ultrastructural findings of Fig. 6C, depicting an MVB associated with the phagosome, in particular support a mechanism whereby exosomes could be reverse endocytosed out of the phagosome as MVBs into the cell, similarly to what was observed with trichosanthin-loaded exosomes (Zhang et al, 2009). From this location, they could hijack the host retrograde trafficking pathway to deliver Leishmania exosomes to the host trans-Golgi (Fig.…”
Section: Leishmania Exosomes Are Delivered To Host Cells and Facilitasupporting
confidence: 55%
“…Second, the ultrastructural findings of Fig. 6C, depicting an MVB associated with the phagosome, in particular support a mechanism whereby exosomes could be reverse endocytosed out of the phagosome as MVBs into the cell, similarly to what was observed with trichosanthin-loaded exosomes (Zhang et al, 2009). From this location, they could hijack the host retrograde trafficking pathway to deliver Leishmania exosomes to the host trans-Golgi (Fig.…”
Section: Leishmania Exosomes Are Delivered To Host Cells and Facilitasupporting
confidence: 55%
“…Endocytosed TCS is incorporated into intraluminal vesicles of the multivesicular body and is then secreted via MVs. The secreted TCS loaded MVs target syngenic and specific allogeneic cells and lead to subsequent internalization, delivery of the toxin into the cytosol and finally resulting in ribosomal inactivation and cell death (64). This shows how a plant toxin can be used as a novel drug approach by using exosome-related pathways.…”
Section: Mvs As Novel Drugs And/or Drug Carriersmentioning
confidence: 99%
“…Trichosanthin (TCS), a plant toxin, is incorporated into intraluminal vesicles of the MVB and is then secreted in association with exosomes upon fusion of the MVB with the plasma membrane [16]. In this paper, we provide evidences that part of CT molecules, once linked to GM1 on the plasma membrane, are sorted to MVB and are secreted as exosomes.…”
Section: Introductionmentioning
confidence: 99%