Microvesicles as Novel Biomarkers and Therapeutic Targets in Transplantation Medicine

Fleißner, Felix; Goerzig, Y.; Haverich, Axel; Thum, Thomas

doi:10.1111/j.1600-6143.2011.03790.x

Cited by 58 publications

(34 citation statements)

References 64 publications

(73 reference statements)

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“…Their lipid bilayer shell could avert proteolytic degradation and thus more effectively transfer signals to target cells (eg, fibroblasts and endothelial cells). In addition, exosomes contain many potential regulatory components including miRNAs, mRNAs, and proteins, which can be transferred as a type of ''physiological lipofection'' to recipient cells to modify their characteristics [71]. This ability to transfer complex messages could explain evidence in previously described experiments where cellular extracts were found to induce epigenetic changes in recipient cells.…”

Section: Discussionmentioning

confidence: 95%

Mesenchymal Stem Cell Exosomes Induce Proliferation and Migration of Normal and Chronic Wound Fibroblasts, and Enhance Angiogenesis In Vitro

Shabbir

Cox

Rodriguez-Menocal

et al. 2015

Stem Cells and Development

563

480

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Although chronic wounds are common and continue to be a major cause of morbidity and mortality, treatments for these conditions are lacking and often ineffective. A large body of evidence exists demonstrating the therapeutic potential of mesenchymal stem cells (MSCs) for repair and regeneration of damaged tissue, including acceleration of cutaneous wound healing. However, the exact mechanisms of wound healing mediated by MSCs are unclear. In this study, we examined the role of MSC exosomes in wound healing. We found that MSC exosomes ranged from 30 to 100-nm in diameter and internalization of MSC exosomes resulted in a dosedependent enhancement of proliferation and migration of fibroblasts derived from normal donors and chronic wound patients. Uptake of MSC exosomes by human umbilical vein endothelial cells also resulted in dosedependent increases of tube formation by endothelial cells. MSC exosomes were found to activate several signaling pathways important in wound healing (Akt, ERK, and STAT3) and induce the expression of a number of growth factors [hepatocyte growth factor (HGF), insulin-like growth factor-1 (IGF1), nerve growth factor (NGF), and stromal-derived growth factor-1 (SDF1)]. These findings represent a promising opportunity to gain insight into how MSCs may mediate wound healing.

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Section: Discussionmentioning

confidence: 95%

Mesenchymal Stem Cell Exosomes Induce Proliferation and Migration of Normal and Chronic Wound Fibroblasts, and Enhance Angiogenesis In Vitro

Shabbir

Cox

Rodriguez-Menocal

et al. 2015

Stem Cells and Development

563

480

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“…The past few years have seen an explosion of research on these shed vesicles, largely because of their perceived roles in cancer, inflammation, coagulation, and stem cell renewal and expansion. Moreover, microvesicle presence in body fluids-including the blood-points to their promise as prospective biomarkers and prognostic indicators in the surveillance of various health conditions (van Doormaal et al 2009;Fleissner et al 2012).…”

mentioning

confidence: 99%

Tumor-derived microvesicles: shedding light on novel microenvironment modulators and prospective cancer biomarkers

D’Souza‐Schorey¹,

Clancy²

2012

Genes Dev.

466

471

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Recent advances in the study of tumor-derived microvesicles reveal new insights into the cellular basis of disease progression and the potential to translate this knowledge into innovative approaches for cancer diagnostics and personalized therapy. Tumor-derived microvesicles are heterogeneous membrane-bound sacs that are shed from the surfaces of tumor cells into the extracellular environment. They have been thought to deposit paracrine information and create paths of least resistance, as well as be taken up by cells in the tumor microenvironment to modulate the molecular makeup and behavior of recipient cells. The complexity of their bioactive cargo—which includes proteins, RNA, microRNA, and DNA—suggests multipronged mechanisms by which microvesicles can condition the extracellular milieu to facilitate disease progression. The formation of these shed vesicles likely involves both a redistribution of surface lipids and the vertical trafficking of cargo to sites of microvesicle biogenesis at the cell surface. Current research also suggests that molecular profiling of these structures could unleash their potential as circulating biomarkers as well as platforms for personalized medicine. Thus, new and improved strategies for microvesicle identification, isolation, and capture will have marked implications in point-of-care diagnostics for cancer patients.

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“…Several groups have reported on whole plasma/bodily fluid profiling of free nucleic acids and proteins as biomarker platforms for monitoring rejection, especially in the context of renal transplantation (6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17). But diagnostic accuracy remains a critical problem, as free nucleic acids and proteins are typically nonspecific and unstable in circulation, requiring a high steady state for reliable quantitation.…”

Section: Introductionmentioning

confidence: 99%

Tissue-specific exosome biomarkers for noninvasively monitoring immunologic rejection of transplanted tissue

Vallabhajosyula¹,

Korutla²,

Habertheuer³

et al. 2017

Journal of Clinical Investigation

137

138

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Microvesicles as Novel Biomarkers and Therapeutic Targets in Transplantation Medicine

Cited by 58 publications

References 64 publications

Mesenchymal Stem Cell Exosomes Induce Proliferation and Migration of Normal and Chronic Wound Fibroblasts, and Enhance Angiogenesis In Vitro

Mesenchymal Stem Cell Exosomes Induce Proliferation and Migration of Normal and Chronic Wound Fibroblasts, and Enhance Angiogenesis In Vitro

Tumor-derived microvesicles: shedding light on novel microenvironment modulators and prospective cancer biomarkers

Tissue-specific exosome biomarkers for noninvasively monitoring immunologic rejection of transplanted tissue

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