A novel strategy for the detection of pyruvate in fermentation processes based on well-distributed enzyme-inorganic hybrid nanoflowers on thiol graphene modified gold electrodes

Lü, Yang; Wu, Nan; Bai, Rongrong; Chen, Minjiao; Dong, Weiliang; Zhou, Jie; Jiang, Min

doi:10.1016/j.electacta.2022.140855

Cited by 7 publications

(10 citation statements)

References 50 publications

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“…Upon optimization, the developed H 2 O 2 and pyruvate biosensor demonstrated a LOD of 0.06 and 0.3 mM (S/N = 3) in the linear range of 0.1-8.2 and 1-8 mM, respectively, as well as good accuracy and sensitivity. 50 In a study conducted by Baek et al, GOx-HRP hNFs-Au NP-decorated GO nanofibers (NFs) was fabricated for the electrochemical glucose biosensor. In this work, firstly, GO NFs were synthesized on the gold chip by the electrospinning method.…”

Section: Electrochemical Detection Based On Hnfsmentioning

confidence: 99%

“…HRP‐hNFs/tG/Au electrode showed enhanced activity and conductivity owing to high catalytic activity and excellent electrochemical performance of the hNFs. Upon optimization, the developed H 2 O 2 and pyruvate biosensor demonstrated a LOD of 0.06 and 0.3 mM (S/N = 3) in the linear range of 0.1–8.2 and 1–8 mM, respectively, as well as good accuracy and sensitivity 50 …”

Section: Application Of Hnfsmentioning

confidence: 99%

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Role of pretty nanoflowers as novel versatile analytical tools for sensing in biomedical and bioanalytical applications

Dadi,

Ocsoy

2024

Smart Medicine

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In recent years, an encouraging breakthrough in the synthesis of immobilized enzymes in flower‐shaped called “organic‐inorganic hybrid nanoflowers (hNFs)” with greatly enhanced catalytic activity and stability were reported. Although, these hNFs were discovered by accident, the enzymes exhibited highly enhanced catalytic activities and stabilities in the hNFs compared with the free and conventionally immobilized enzymes. Herein, we rationally utilized the catalytic activity of the hNFs for analytical applications. In this comprehensive review, we covered the design and use of the hNFs as novel versatile sensors for electrochemical, colorimetric/optical and immunosensors‐based detection strategies in analytical perspective.

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Section: Electrochemical Detection Based On Hnfsmentioning

confidence: 99%

Section: Application Of Hnfsmentioning

confidence: 99%

Role of pretty nanoflowers as novel versatile analytical tools for sensing in biomedical and bioanalytical applications

Dadi,

Ocsoy

2024

Smart Medicine

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“…According to the data in Table 3, the parameters of the Cu 3 (PO 4 ) 2 unit cell (triclinic crystal system) were calculated as follows: a = 0.4857 nm, b = 0.5292 nm, c = 0.6159 nm, α = 72.48°, β = 87.02°, γ = 68.46° and V = 0.1401 nm 3 . Let us compare these values with the results of Shoemaker et al [33]: a = 0.4853 nm, b = 0.5285 nm, c = 0.6182 nm, α = 72.35°, β = 86.99°, γ = 68.54° and V = 0.1403 nm 3 . As could be seen, the minimum difference between the parameters of this work and those published in the literature [33] is 0.03%, and the maximum is 0.37%.…”

Section: Physicochemical Analysis Of Cu 3 (Po 4 ) 2 •3h 2 Omentioning

confidence: 99%

“…In the past few years, copper (II) orthophosphate trihydrate Cu 3 (PO 4 ) 2 •3H 2 O has gained a significant increase of the scientific interest. Thus, a number of promising composites have been obtained with the participation of this compound: a colorimetric platform for glucose detection [1], a colorimetric biosensor for epinephrine identification [2], a nanomaterial for pyruvate specification in fermentation processes [3], a catalyst for olive oil hydrolysis [4], a surfactant [5], an enzyme immobilizer [6] and a nanocatalyst for the hydrolysis of allergenic cow᾽s milk proteins [7].…”

Section: Introductionmentioning

confidence: 99%

Thermodynamic and thermochemical properties of Cu3(PO4)2·3H2O

Kozma¹,

Golub²,

Golub³

et al. 2023

chemija

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Copper (II) orthophosphate trihydrate Cu3(PO4)2·3H2O has been synthesized by the exchange chemical reactions in aqueous solutions from the compounds Cu(NO3)2·3H2O, NH4OH and H3PO4. The final product has been investigated by using atomic absorption spectroscopy (AAS), infrared spectrometry (IRS), differential thermal analysis (DTA) and X-ray powder diffraction (XRD) techniques. For the first time, the experimental heat capacity Ср° of Cu3(PO4)2·3H2O has been investigated in the temperature range 198‒402 K. Combining the experimental results with the literature data made it possible to determine the thermodynamic and thermochemical properties, such as enthalpy НТ° , entropy SТ° , Gibbs energy GТ° , enthalpy of formation ΔHf°(T), Gibbs energy of formation ΔGf°(T) and logarithm of the equilibrium constant of formation reaction log[Kf°(T)], for the title compound. It was found that at the extreme temperatures of the experiment, Cu3(PO4)2·3H2O has Ср° = 245.96 J·mol–1·K–1, Н° = –3169.59 kJ·mol–1, S° = 311.34 J·mol–1·K–1, G° = –3231.24 kJ·mol–1 (at 198 K); Ср° = 455.31 J·mol–1·K–1, Н° = –3097.93 kJ·mol–1, S° = 551.48 J·mol–1·K–1, G° = –3319.62 kJ·mol–1, ΔHf° = –3138.48 kJ·mol–1, ΔGf° = –2625.39 kJ·mol–1, log[K ] = 341.13 (at 402 K).

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“…In spite of the long history of S. cerevisiae as an industrial microorganism, the problems related to pyruvate transport, distribution, and fluctuation in different subcellular compartmentalizations are still not completely understood. , Standard methods to measure pyruvate have been reported, such as amperometric, spectrophotometric, and high-performance liquid chromatography (HPLC) techniques. , However, these methods only allow the detection of extracellular pyruvate and cannot reflect the physiological state of microbial cells in real time . Obtaining spatiotemporal information about metabolites through these traditional monitoring methods after cell lysis is time-consuming and incompatible with the study of spatiotemporal dynamics in single and intact cells …”

Section: Introductionmentioning

confidence: 99%

Lighting up Pyruvate Metabolism in Saccharomyces cerevisiae by a Genetically Encoded Fluorescent Biosensor

Yang,

Jia,

Xie

et al. 2024

J. Agric. Food Chem.

Self Cite

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Monitoring intracellular pyruvate is useful for the exploration of fundamental metabolism and for guiding the construction of yeast cell factories for chemical production. Here, we employed a genetically encoded fluorescent Pyronic biosensor to light up the pyruvate metabolic state in the cytoplasm, nucleus, and mitochondria of Saccharomyces cerevisiae BY4741. A strong correlation was observed between the pyruvate fluctuation in mitochondria and cytoplasm when exposed to different metabolites. Further metabolic analysis of pyruvate uptake and glycolytic dynamics showed that glucose and fructose dose-dependently activated cytoplasmic pyruvate levels more effectively than direct exposure to pyruvate. Meanwhile, the Pyronic biosensor could visually distinguish phenotypes of the wild-type S. cerevisiae BY4741 and the pyruvate-hyperproducing S. cerevisiae TAM at a single-cell resolution, having the potential for high-throughput screening. Overall, Pyronic biosensors targeting different suborganelles contribute to mapping and studying the central carbon metabolism in-depth and guide the design and construction of yeast cell factories.

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A novel strategy for the detection of pyruvate in fermentation processes based on well-distributed enzyme-inorganic hybrid nanoflowers on thiol graphene modified gold electrodes

Cited by 7 publications

References 50 publications

Role of pretty nanoflowers as novel versatile analytical tools for sensing in biomedical and bioanalytical applications

Role of pretty nanoflowers as novel versatile analytical tools for sensing in biomedical and bioanalytical applications

Thermodynamic and thermochemical properties of Cu3(PO4)2·3H2O

Lighting up Pyruvate Metabolism in Saccharomyces cerevisiae by a Genetically Encoded Fluorescent Biosensor

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