A novel strategy for detection of small molecules based on aptamer/gold nanoparticles/graphitic carbon nitride nanosheets as fluorescent biosensor

Shirani, Maryam; Kalantari‬, Heibatullah; Khodayar, Mohammad Javad; Kouchak, Maryam; Rahbar, Nadereh

doi:10.1016/j.talanta.2020.121235

Cited by 29 publications

(9 citation statements)

References 50 publications

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“…Bulk CN was prepared according to the previous procedure through the pyrolysis of melamine in the air atmosphere [25]. In detail, a crucible containing 15 g of melamine was placed in a Muffle Furnace.…”

Section: Synthesis Of G-cn Nanzosheetsmentioning

confidence: 99%

An Electrochemiluminescence Biosensor for the Detection of Alzheimer’s Tau Protein Based on Gold Nanostar Decorated Carbon Nitride Nanosheets

et al. 2022

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Human Tau protein is the most reliable biomarker for the prediction of Alzheimer’s disease (AD). However, the assay to detect low concentrations of tau protein in serum is a great challenge for the early diagnosis of AD. This paper reports an electrochemiluminescence (ECL) immunosensor for Tau protein in serum samples. Gold nanostars (AuNSs) decorated on carbon nitride nanosheets (AuNS@g-CN nanostructure) show highly strong and stable ECL activity compared to pristine CN nanosheets due to the electrocatalytic and surface plasmon effects of AuNSs. As a result of the strong electromagnetic field at branches, AuNSs showed a better ECL enhancement effect than their spherical counterpart. For the fabrication of a specific immunosensor, immobilized AuNSs were functionalized with a monoclonal antibody specific for Tau protein. In the presence of Tau protein, the ECL intensity of the immunosensor decreased considerably. Under the optimal conditions, this ECL based immunosensor exhibits a dynamic linear range from 0.1 to 100 ng mL−1 with a low limit of detection of 0.034 ng mL−1. The LOD is less than the Tau level in human serum; thus, this study provides a useful method for the determination of Tau. The fabricated ECL immunosensor was successfully applied to the detection of Tau, the biomarker in serum samples. Therefore, the present approach is very promising for application in diagnosing AD within the early stages of the disease.

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Section: Synthesis Of G-cn Nanzosheetsmentioning

confidence: 99%

An Electrochemiluminescence Biosensor for the Detection of Alzheimer’s Tau Protein Based on Gold Nanostar Decorated Carbon Nitride Nanosheets

et al. 2022

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“…Some studies have focused on detecting several targets based on the aptamer-competitive quenching of carbon quantum dots and graphene materials [120][121][122][123][124][125]. Recently, GO was used as a "nanoquencher" for fluorescent materials via the FRET strategy with ssDNA as a nanoscaffold, capable of adsorbing to the GO surface through π-π stacking interactions with the nucleotide bases [126][127][128][129].…”

Section: Fluorescence Aptasensormentioning

confidence: 99%

“…Shirania et al proposed a fluorometric aptasensor for assaying digoxin (DGX) based on the quenching effect of an aptamer/gold nanoparticle probe on the fluorescence intensity of graphitic carbon nitride nanosheets. The FRET strategy was employed, and the presence of DGX resulted in fluorescence intensity restoration (Figure 6B), with concentrations ranging from 10 to 500 ng/L and a LOD of 3.2 ng/L [120]. Tan et al [121] proposed another protocol system that uses a ratiometric fluorescent nanoprobe, in which dual emission at 518 and 608 nm is induced by a single excitation.…”

Section: Fluorescence Aptasensormentioning

confidence: 99%

“…(A)Schematics showing the preparation of PAA@Arg@ATT-AuNC as a fluorescent probe for trichothecenes A (T-2) toxin assay, reproduced from[119]. (B) Graphical illustration for the assaying of digoxin (DGX) using the aptamer/AuNPs/g-C3N4NS sensor probe, reproduced from[120]. (C) Schematic illustration of the zearalenone assay using a ratiometric fluorescent nanoprobe with dual emission at 518 and 608 nm, reproduced from[121].…”

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Recent Advances in Aptamer Sensors

Shaban

Kim

2021

Sensors

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Recently, aptamers have attracted attention in the biosensing field as signal recognition elements because of their high binding affinity toward specific targets such as proteins, cells, small molecules, and even metal ions, antibodies for which are difficult to obtain. Aptamers are single oligonucleotides generated by in vitro selection mechanisms via the systematic evolution of ligand exponential enrichment (SELEX) process. In addition to their high binding affinity, aptamers can be easily functionalized and engineered, providing several signaling modes such as colorimetric, fluorometric, and electrochemical, in what are known as aptasensors. In this review, recent advances in aptasensors as powerful biosensor probes that could be used in different fields, including environmental monitoring, clinical diagnosis, and drug monitoring, are described. Advances in aptamer-based colorimetric, fluorometric, and electrochemical aptasensing with their advantages and disadvantages are summarized and critically discussed. Additionally, future prospects are pointed out to facilitate the development of aptasensor technology for different targets.

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“…have developed a novel apta‐biosensor based on the FRET phenomenon between g‐C 3 N 4 NS and AuNPs (Figure 10B‐D). [ 122 ] The authors fabricated an ultra‐sensitive platform for the trace level detection of DGX in human plasma samples without any further sample pre‐treatment steps. The developed nano‐biosensor showed high selectivity, simplicity, low cost, a short assay time, a wide dynamic linear range, and considered green.…”

Section: Applications Of G‐c3n4 In Signal‐transducing Agent For Sensomentioning

confidence: 99%

Emerging tri‐s‐triazine‐based graphitic carbon nitride: A potential signal‐transducing nanostructured material for sensor applications

et al. 2020

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Today, tris‐s‐triazine based graphitic carbon nitride (g‐C3N4) is a new research hot topic. It has a unique electronic band structure, high physicochemical stability, large surface area, and is “earth‐abundant.” These and other properties have made it a highly researched material especially for visible light photocatalysis and photodegradation applications and as the starting material from which to develop novel electrochemical sensing platforms. In this review, the state‐of‐the‐art technologies utilizing tris‐s‐triazine graphitic carbon nitride as a tailorable signal‐transducing nanostructured material for sensing applications is presented in detail. Initially, the electronic structure of g‐C3N4, morphologies, doping, heterojunctions, its combination with other carbon materials, and defect formation, is described, which is followed by a discussion on its role in electrochemiluminescence, photoelectrochemical, fluorescence sensors and gas sensors as a signal transducer with appropriate examples. This review concludes with a discussion summarizing state‐of‐the‐art and both future perspectives and challenges at the cutting edge of this research.

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A novel strategy for detection of small molecules based on aptamer/gold nanoparticles/graphitic carbon nitride nanosheets as fluorescent biosensor

Cited by 29 publications

References 50 publications

An Electrochemiluminescence Biosensor for the Detection of Alzheimer’s Tau Protein Based on Gold Nanostar Decorated Carbon Nitride Nanosheets

An Electrochemiluminescence Biosensor for the Detection of Alzheimer’s Tau Protein Based on Gold Nanostar Decorated Carbon Nitride Nanosheets

Recent Advances in Aptamer Sensors

Emerging tri‐s‐triazine‐based graphitic carbon nitride: A potential signal‐transducing nanostructured material for sensor applications

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