A novel strain Lactobacillus brevis 8-2B inhibiting Aspergillus carbonarius growth and ochratoxin a production

Li, Li; Yang, Bolei; Humza, Muhammad; Geng, Hairong; Wang, Gang; Zhang, Chenxi; Gao, Sheng; Xing, Fuguo; Liu, Yang

doi:10.1016/j.lwt.2020.110308

Cited by 12 publications

(6 citation statements)

References 29 publications

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“…As previously reported, the antifungal mechanism of weak organic acids is hypothesised to focus on the plasma membrane (Freese et al ., 1973; Cao et al ., 2021; Li et al ., 2021). This study corroborates the concept that the low pH and the multiple acids produced by L. plantarum 124 could destroy the plasma membrane, resulting in notable morphological changes in fungal mycelia and the formation of membrane‐bound vesicles inside the cells.…”

Section: Resultsmentioning

confidence: 99%

Effect of antifungal compounds secreted by Lactiplantibacillus plantarum 124 against Aspergillus flavus and Penicillium sp. and its application in Kaeng‐Tai‐Pla‐Haeng to extend the shelf life

Kanjan

Sakpetch

2023

Int J of Food Sci Tech

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SummaryAspergillus flavus and Penicillium spp. are the most common spoilage fungi in the ready‐to‐eat, intermediate‐moisture Thai curry of Kaeng‐Tai‐Pla‐Haeng, which causes serious economic loss and public health concerns. The use of lactic acid bacteria (LAB) with antifungal activity has been a growing interest in food bio‐preservation. In this study, the antifungal efficacy of Lactiplantibacillus plantarum 124 (L. plantarum 124) against the spoilage fungi Aspergillus flavus and Penicillium sp. was evaluated. The cell‐free supernatants (CFSs) of L. plantarum 124 showed strong inhibition against A. flavus and Penicillium sp., with MIC ranging from 11.25 to 22.5 mg/mL. The significant loss of the antifungal activity was observed by pH neutralization, whereas either heat or proteinase K treatment showed no influence. The major compounds present in CFSs were lactic acid (49.57%) and acetic acid (25.85%). Others, such as 2,3‐dimethyl‐5‐ethylpyrazine (6.07%), 2,5‐dimethyl‐3‐propylpyrazine (2.10%), Cyclo(D‐Leu‐L‐Pro) (2.30%), Ethyl 5‐oxopyrrolidine‐3‐carboxylate (1.84%), and palmitic acid (1.80%) were detected at low relative amount. As observed by electron microscopy, the CFSs secreted by L. plantarum 124 caused severe damage to cell membranes, and organelles of A. flavus and Penicillium sp. In addition, L. plantarum 124 supernatant demonstrated no cytotoxicity on Vero cells and it could extend the food product's shelf‐life from 7 days to 21 days without any commercial preservatives. These results demonstrate that antifungal metabolites secreted by L. plantarum 124 are candidates for the development of bio‐preservative agents.

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Section: Resultsmentioning

confidence: 99%

Effect of antifungal compounds secreted by Lactiplantibacillus plantarum 124 against Aspergillus flavus and Penicillium sp. and its application in Kaeng‐Tai‐Pla‐Haeng to extend the shelf life

Kanjan

Sakpetch

2023

Int J of Food Sci Tech

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“…Strains of Lacticaseibacillus casei and Lactobacillus acidophilus have also shown to have inhibition effects against A. parasiticus ( Abbaszadeh et al, 2015 ). LAB have been reported to produce compounds during growth with potent antifungal effects which may damage the fungal membrane and cell wall structure, resulting in lysis of hyphae and spores ( de Souza de Azevedo et al, 2020 ; Li et al, 2021 ). As indicated by the two first assays, L. brevis 2QB422 was the most effective strain in suppressing the growth of A. parasiticus , which reached a maximum diameter of 4.91 ± 0.6 cm ( Table 3 ; Figure 1 ), followed by the range of 5.26–5.46 cm for strains 3QB361 ( Lactiplantibacillus plantarum ), 3QB398 ( Levilactobacillus spp.…”

Section: Resultsmentioning

confidence: 99%

Effect of Lactic Acid Bacteria Strains on the Growth and Aflatoxin Production Potential of Aspergillus parasiticus, and Their Ability to Bind Aflatoxin B1, Ochratoxin A, and Zearalenone in vitro

et al. 2021

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The increased consumption of plant-based foods has intensified the concern related to mycotoxin intoxication. This study aimed to investigate the effect of selected lactic acid bacteria (LAB) strains on the growth of Aspergillus parasiticus NRRL 2999 and its production of aflatoxin (AF). The ability of the heat-killed (100°C for 1 h) LAB strains to bind aflatoxin M1 (AFM1) in milk and aflatoxin B1 (AFB1), ochratoxin A (OTA), and zearalenone (ZEN) in potassium phosphate buffer (PPB) was also evaluated in vitro. Ten LAB strains were tested individually, by inoculating them simultaneously with the fungus or after incubation of the fungus for 24 or 48 h at 25°C. Double layer yeast extract sucrose (YES) agar, de Man Rogosa and Sharpe (MRS) agar, and YES broth were incubated for 7 days at 25°C to follow the development of the fungus. Levilactobacillus spp. 3QB398 and Levilactobacillus brevis 2QB422 strains were able to delay the growth of A. parasiticus in YES broth, even when these strains were inoculated 24 h after the fungus. The inhibitory effect of these LAB strains was confirmed by the reduction of fungus colony size, suggesting dominance of LAB by competition (a Lotka-Voltera effect). The production of AFB1 by A. parasiticus was inhibited when the fungus was inoculated simultaneously with Lactiplantibacillus plantarum 3QB361 or L. plantarum 3QB350. No AFB1 was found when Levilactobacillus spp. 2QB383 was present, even when the LAB was inoculated 48 h after the fungus. In binding studies, seven inactivated LAB strains were able to promote a reduction of at least 50% the level of AFB1, OTA, and ZEN. This reduction varied depending on the pH of the PPB. In milk, however, only two inactivated LAB strains were able to reduce AFM1, with a reduction of 33 and 45% for Levilactobacillus spp. 3QB398 (Levilactobacillus spp.) and L. brevis 2QB422, respectively. Nevertheless, these results clearly indicate the potential of using LAB for mycotoxin reduction.

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“…L. brevis enjoys GRAS status (Generally Recognized As Safe) on the basis of their widespread historical use in various traditional fermented food products [ 48 ]. Strains of L. brevis can vary significantly with some possessing antipathogenic [ 49 ], antioxidant, immunomodulatory [ 50 ], anticarcinogenic [ 51 ] and antifungal [ 52 ] activity, all considered probiotic properties.…”

Section: Discussionmentioning

confidence: 99%

The effects of Levilactobacillus brevis on the physiological parameters and gut microbiota composition of rats subjected to desynchronosis

et al. 2021

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Background All living organisms have developed during evolution complex time-keeping biological clocks that allowed them to stay attuned to their environments. Circadian rhythms cycle on a near 24 h clock. These encompass a variety of changes in the body ranging from blood hormone levels to metabolism, to the gut microbiota composition and others. The gut microbiota, in return, influences the host stress response and the physiological changes associated with it, which makes it an important determinant of health. Lactobacilli are traditionally consumed for their prophylactic and therapeutic benefits against various diseases, namely, the inflammatory bowel syndrome, and even emerged recently as promising psychobiotics. However, the potential role of lactobacilli in the normalization of circadian rhythms has not been addressed. Results Two-month-old male rats were randomly divided into three groups and housed under three different light/dark cycles for three months: natural light, constant light and constant darkness. The strain Levilactobacillus brevis 47f was administered to rats at a dose of 0.5 ml per rat for one month and The rats were observed for the following two months. As a result, we identified the biomarkers associated with intake of L. brevis 47f. Changing the light regime for three months depleted the reserves of the main buffer in the cell—reduced glutathione. Intake of L. brevis 47f for 30 days restored cellular reserves of reduced glutathione and promoted redox balance. Our results indicate that the levels of urinary catecholamines correlated with light/dark cycles and were influenced by intake of L. brevis 47f. The gut microbiota of rats was also influenced by these factors. L. brevis 47f intake was associated with an increase in the relative abundance of Faecalibacterium and Roseburia and a decrease in the relative abundance of Prevotella and Bacteroides. Conclusions The results of this study show that oral administration of L. brevis 47f, for one month, to rats housed under abnormal lightning conditions (constant light or constant darkness) normalized their physiological parameters and promoted the gut microbiome's balance.

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A novel strain Lactobacillus brevis 8-2B inhibiting Aspergillus carbonarius growth and ochratoxin a production

Cited by 12 publications

References 29 publications

Effect of antifungal compounds secreted by Lactiplantibacillus plantarum 124 against Aspergillus flavus and Penicillium sp. and its application in Kaeng‐Tai‐Pla‐Haeng to extend the shelf life

Effect of antifungal compounds secreted by Lactiplantibacillus plantarum 124 against Aspergillus flavus and Penicillium sp. and its application in Kaeng‐Tai‐Pla‐Haeng to extend the shelf life

Effect of Lactic Acid Bacteria Strains on the Growth and Aflatoxin Production Potential of Aspergillus parasiticus, and Their Ability to Bind Aflatoxin B1, Ochratoxin A, and Zearalenone in vitro

The effects of Levilactobacillus brevis on the physiological parameters and gut microbiota composition of rats subjected to desynchronosis

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