The effects of Levilactobacillus brevis on the physiological parameters and gut microbiota composition of rats subjected to desynchronosis

Abstract

Background All living organisms have developed during evolution complex time-keeping biological clocks that allowed them to stay attuned to their environments. Circadian rhythms cycle on a near 24 h clock. These encompass a variety of changes in the body ranging from blood hormone levels to metabolism, to the gut microbiota composition and others. The gut microbiota, in return, influences the host stress response and the physiological changes associated with it, which makes it an important determinant of health. Lactobacilli are traditionally consumed for their prophylactic and therapeutic benefits against various diseases, namely, the inflammatory bowel syndrome, and even emerged recently as promising psychobiotics. However, the potential role of lactobacilli in the normalization of circadian rhythms has not been addressed. Results Two-month-old male rats were randomly divided into three groups and housed under three different light/dark cycles for three months: natural light, constant light and constant darkness. The strain Levilactobacillus brevis 47f was administered to rats at a dose of 0.5 ml per rat for one month and The rats were observed for the following two months. As a result, we identified the biomarkers associated with intake of L. brevis 47f. Changing the light regime for three months depleted the reserves of the main buffer in the cell—reduced glutathione. Intake of L. brevis 47f for 30 days restored cellular reserves of reduced glutathione and promoted redox balance. Our results indicate that the levels of urinary catecholamines correlated with light/dark cycles and were influenced by intake of L. brevis 47f. The gut microbiota of rats was also influenced by these factors. L. brevis 47f intake was associated with an increase in the relative abundance of Faecalibacterium and Roseburia and a decrease in the relative abundance of Prevotella and Bacteroides. Conclusions The results of this study show that oral administration of L. brevis 47f, for one month, to rats housed under abnormal lightning conditions (constant light or constant darkness) normalized their physiological parameters and promoted the gut microbiome's balance.

Homeostatic crosstalk among gut microbiome, hypothalamic and hepatic circadian clock oscillations, immunity and metabolism in response to different light–dark cycles: A multiomics study

“…71 Moreover, it has been reported that intestinal gram-positive bacteria can regulate the CC of hepatic biological functions during the light phase. 72 Previous studies have also shown that specific bacteria, such as Prevotella, Faecalibacterium, Roseburia, Akkermansia, Bifidobacterium, and Allobaculum, are highly correlated with the host's CC and hepatic metabolism, 73,74 while Veillonella, Lactobacillus, Streptococcus, Oribacterium, Rothia, and Actinomyces are closely related to sleep disorders. 75 In conclusion, our results revealed that the GM can play a regulatory role in modifying hepatic CC homeostasis disorder, providing potential targets for the development of probiotics to address circadian disruption in individuals such as shift workers.…”

Drosophila<em> melanogaster</em> as a Translational Model System to Explore the Impact of Phytochemicals on Human Health

“…Among them, L. brevis belonging to the Lactobacillaceae family was 4-fold higher in flies reared on pepper-containing diets than in the guts of flies raised on the control diet. L. brevis is traditionally consumed for its prophylactic and therapeutic benefits against various diseases, such as human inflammatory bowel syndrome [102].…”

“…Among them, L. brevis belonging to the Lactobacillaceae family was 4-fold higher in flies reared on pepper-containing diets than in the guts of flies raised on the control diet. L. brevis is traditionally consumed for its prophylactic and therapeutic benefits against various diseases, such as human inflammatory bowel syndrome [102]. Moreover, Jimenez-Padilla et al [103] found that Drosophila flies fed diets with strawberries and blueberries had an increased abundance of Acetobacter in their microbiome.…”

Surface Layer Protein Pattern of Levilactobacillus brevis Strains Investigated by Proteomics

“…Levilactobacillus brevis is a lactic acid bacterium (LAB) that produces Slps. This microorganism is present in many different environments, such as sauerkraut, sourdough, silage, milk, cheese, mouth and intestinal tract of humans and animals, and is involved in the production of a wide spectrum of fermented products [13][14][15][16][17]. Interestingly, the potential involvement of Slps in its probiotic and biotechnological features was documented [7,[18][19][20][21][22][23].…”