A novel stationary phase for the high performance liquid chromatographic separation and determination of phenols

Penner, Natasha; Nesterenko, Pavel N.; Khryaschevsky, Andrey V.; Stranadko, Tatiana N.; Шпигун, О. А.

doi:10.1070/mc1998v008n01abeh000895

Cited by 14 publications

(7 citation statements)

References 11 publications

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“…Similar dependences of logk on X were obtained for the retention of phenol and some of its chloro-, nitro-and methyl-substituted derivatives on HC-PS MN-200. 18 This can be attributed to the complex mechanism of the retention of dihydroxybenzenes and aminophenols on HC-PS, which includes hydrophobic and p-p interactions of the phenols with the polystyrene matrix, size exclusion in micropores and the formation of relatively stable adducts with the organic solvent in the eluent. The main feature of the HC-PS stationary phase is its high affinity for the studied analytes.…”

Section: Effect Of Acetonitrile Contentmentioning

confidence: 99%

“…Recently, unmodified hypercrosslinked polystyrene (HC2PS) was shown to be a promising stationary phase for RP-HPLC. 18,19 HC2PS has a hydrophobic surface and displays a high affinity for phenyl groups in the molecules of the analytes as the retention mechanism on this stationary phase includes not only hydrophobic but also strong p-p interactions. [18][19][20] .…”

Section: Introductionmentioning

confidence: 99%

“…18,19 HC2PS has a hydrophobic surface and displays a high affinity for phenyl groups in the molecules of the analytes as the retention mechanism on this stationary phase includes not only hydrophobic but also strong p-p interactions. [18][19][20] . These properties should result in enhanced retention and selectivity of unmodified HC2PS to hydrophilic aromatic molecules such as dihydroxybenzenes, aminophenols and phenylenediamines.…”

Section: Introductionmentioning

confidence: 99%

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Simultaneous determination of dihydroxybenzenes, aminophenols and phenylenediamines in hair dyes by high-performance liquid chromatography on hypercross-linked polystyrene

Penner

Nesterenko

2000

Analyst

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The retention of polar organic molecules such as dihydroxybenzenes, aminophenols and phenylenediamines on a 250 x 4.6 mm id column packed with 5 microns hypercross-linked polystyrene Chromalite 5HGN (Purolite) was studied. The influence of separation parameters such as concentration of acetonitrile, buffer (citrate, phosphate) concentration, ionic strength and pH of the eluent on their retention was investigated. Under optimum conditions [acetonitrile-0.3 mol l-1 ammonium phosphate, pH 5.15 (30:70 v/v)], eight substances generally used as dye intermediates in hair colouring compositions could be separated within 20 min. An HPLC method with spectrophotometric detection is proposed for the simultaneous determination of pyrocatechol, resorcinol, hydroquinone, o-, m- and p-aminophenols and p-phenylenediamine in commercial haircolour products. The detection limits of these compounds are in the range 0.05-0.16 microgram ml-1. The suitability of the method is demonstrated by the analysis of three different permanent hair dyes.

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Section: Effect Of Acetonitrile Contentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Simultaneous determination of dihydroxybenzenes, aminophenols and phenylenediamines in hair dyes by high-performance liquid chromatography on hypercross-linked polystyrene

Penner

Nesterenko

2000

Analyst

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“…As the polymer surface has no polar sites, PS-DVB stationary phases have been successfully used for the separation of peptides, proteins and DNA fragments [3][4][5]. In recent years there has been growing interest in the application of PS-DVB adsorbents for the RPHPLC of small molecules, such as phenol and its derivatives [6][7][8] and polyaromatic hydrocarbons [3]. For the latter compounds the enhanced affinity of the polymer towards aromatic compounds improves the selectivity of their separation [9].…”

Section: Introductionmentioning

confidence: 99%

“…Experimental Styrosorb material, functionalized with hydrophilic tris-groups on the outer surface of the beads, has been characterized as a restricted-access packing for RPHPLC [ 19]. A recent application of hypercrosslinked polystyrene with a hydrophobic surface to the determination of phenols included preconcentration then chromatographic separation on the same column [6]. Both untreated MN-200 resin and the same resin impregnated with metallochromic organic reagents have also been used for efficient separation of alkaline earth and transition metal ions [20,21].…”

Section: Introductionmentioning

confidence: 99%

Investigation of the properties of hypercrosslinked polystyrene as a stationary phase for high-performance liquid chromatography

et al. 1999

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SummaryThe mechanical rigidity, swelling properties, adsorption selectivity, and chromatographic performance of hypercrosslinked polystyrene (mainly MN-200 or Purosep-200; Purolite, UK) have been studied to evaluate the use of the material as a stationary phase for reversedphase high-performance liquid chromatography (RPHPLC). By use of inverse size-exclusion chromatography (SEC) this adsorbent, with a high specific 2 1 surface area of 1500 m g was found to have a biporous structure with micropores of ca 1-2 nm and macropores ca 100 nm in diameter. The polymer does not change its volume significantly on changing water for organic solvents. The retention increments for methylene and phenyl groups were calculated and indicated that the mechanism of retention on the hypercrosslinked polystyrene involves re-re interactions and strong hydrophobic interactions. The column performance of the hypercrosslinked polystyrene was found to be acceptable, with reduced plate height increasing very slowly as the linear velocity of the mobile phase increased to high values (up to 20-45 cm min-1). Columns containing hypercrosslinked polystyrene were evaluated for the separation of phenols, dialkyl phthalates, and polyaromatic compounds. On-column preconcentration of trace organic compounds from aqueous media is possible. With smaller particles of hypercrosslinked polystyrene becoming available, this material can be regarded as an alternative to alkylsilica as a hydrolytically stable column-packing material for RPHPLC.

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Analytical Aspects of Phenolic Compounds

Zabicky

2009

Patai's Chemistry of Functional Groups

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Introduction Gas Chromatography Liquid Chromatography Electrophoresis Biosensors Electrochemical Methods Ultraviolet‐Visible Detection Methods Infrared and Raman Spectral Methods Nuclear Magnetic Resonance Mass Spectrometry Miscellaneous Methods

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A novel stationary phase for the high performance liquid chromatographic separation and determination of phenols

Cited by 14 publications

References 11 publications

Simultaneous determination of dihydroxybenzenes, aminophenols and phenylenediamines in hair dyes by high-performance liquid chromatography on hypercross-linked polystyrene

Simultaneous determination of dihydroxybenzenes, aminophenols and phenylenediamines in hair dyes by high-performance liquid chromatography on hypercross-linked polystyrene

Investigation of the properties of hypercrosslinked polystyrene as a stationary phase for high-performance liquid chromatography

Analytical Aspects of Phenolic Compounds

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