Simultaneous determination of dihydroxybenzenes, aminophenols and phenylenediamines in hair dyes by high-performance liquid chromatography on hypercross-linked polystyrene

Abstract

The retention of polar organic molecules such as dihydroxybenzenes, aminophenols and phenylenediamines on a 250 x 4.6 mm id column packed with 5 microns hypercross-linked polystyrene Chromalite 5HGN (Purolite) was studied. The influence of separation parameters such as concentration of acetonitrile, buffer (citrate, phosphate) concentration, ionic strength and pH of the eluent on their retention was investigated. Under optimum conditions [acetonitrile-0.3 mol l-1 ammonium phosphate, pH 5.15 (30:70 v/v)], eight substances generally used as dye intermediates in hair colouring compositions could be separated within 20 min. An HPLC method with spectrophotometric detection is proposed for the simultaneous determination of pyrocatechol, resorcinol, hydroquinone, o-, m- and p-aminophenols and p-phenylenediamine in commercial haircolour products. The detection limits of these compounds are in the range 0.05-0.16 microgram ml-1. The suitability of the method is demonstrated by the analysis of three different permanent hair dyes.

Elucidation of retention mechanisms on hypercrosslinked polystyrene used as column packing material for high-performance liquid chromatography

“…However, there are only a few examples illustrating the applicability of this material in the capacity of stationary phases in HPLC [6][7][8][9].…”

Application of silica-based hyper-crosslinked sulfonate-modified reversed stationary phases for separating highly hydrophilic basic compounds

“…Therefore, the separation and quantitation of 4-aminophenol in the presence of a large amount of p-acetamidophenol is of great importance and RPLC is one of the most frequently used techniques for their analysis. Because of its extreme hydrophilicity, 4-aminophenol is almost always separated at a pH greater than the pK a (5.50) of the anilinium group to achieve higher retention [18][19][20][21]. Nevertheless, even the retention of the uncharged form is usually still insufficient.…”

“…However, the addition of strong ion pairing reagents to the eluent is not desirable because (1) it makes the separation incompatible with mass spectrometric detection, (2) it is hard to recover the column after use since these additives tend to stick very strongly to the stationary phase, and (3) it is not practical in preparative separations. Another approach to increasing retention of highly hydrophilic basic compounds is to separate them as free bases at high pH [18][19][20][21]. However, most commercial silica-based stationary phases are not sufficiently stable at high pH to allow alkylamines to be deprotonated Cation-exchange chromartography has also been used for the separation of highly hydrophilic basic compounds [22][23][24][25].…”

Whole-cell biosensing of 3-chlorocatechol in liquids and soils

“…Effects of the mobile phase, including buffer composition and concentration, on HLPC detection of CC have also been investigated [32]. HPLC detection of CC in soils and soil extracts has not, however, been described previously.…”

“…High-performance liquid chromatography has been used to analyze (chloro-) catechols in a variety of sample types, including urine, water, and dye [16,17,32]. Effects of the mobile phase, including buffer composition and concentration, on HLPC detection of CC have also been investigated [32].…”