A novel solution for freezing small numbers of spermatozoa using a sperm vitrification device

Berkovitz, Arie; Miller, Netanella; Silberman, M.; Belenky, Michael; Itsykson, Pavel

doi:10.1093/humrep/dey304

Cited by 55 publications

(49 citation statements)

References 18 publications

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“…To freeze a very small number of human spermatozoa, a multi-well mini plate has been newly developed for vitrification using~1 μl droplets. Sperm frozen using this method showed descent recovery rates and post thaw motility [48]. This novel approach was commented by Paffoni and Palini [49] [49].…”

Section: Does Vitrification Have To Be Ultra-fast Freezing?mentioning

confidence: 79%

Human sperm vitrification: the state of the art

Tao

Sanger

Saewu

et al. 2020

Reprod Biol Endocrinol

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Sperm cryopreservation has been widely used in assisted reproductive technology (ART) and has resulted in millions of live births. Two principal approaches have been adopted: conventional (slow) freezing and vitrification. As a traditional technique, slow freezing has been successfully employed and widely used at ART clinics whereas the latter, a process to solidify liquid into an amorphous or glassy state, may become a faster alternative method of sperm cryopreservation with significant benefits in regard to simple equipment and applicability to fertility centers. Sperm vitrification has its own limitations. Firstly, small volume of load is usually plunged to liquid nitrogen to achieve high cooling rate, which makes large volume sample cryopreservation less feasible. Secondly, direct contact with liquid nitrogen increases the potential risk of contamination. Recently, new carriers have been developed to facilitate improved control over the volume and speed, and new strategies have been implemented to minimize the contamination risk. In summary, although sperm vitrification has not yet been applied in routine sperm cryopreservation, its potential as a standard procedure is growing.

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Section: Does Vitrification Have To Be Ultra-fast Freezing?mentioning

confidence: 79%

Human sperm vitrification: the state of the art

Tao

Sanger

Saewu

et al. 2020

Reprod Biol Endocrinol

View full text Add to dashboard Cite

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“…To overcome these challenges, hollow capsules similar to empty ZP have been fabricated to freeze small numbers of sperms. The novel sperm vitrification device [43] 2018 Ejaculate 96% 33% NA 59%…”

Section: Single-sperm Cryopreservation Within Non-biological Carriersmentioning

confidence: 99%

“…The fertilization and pregnancy rates were reported to be 59% and 55%, respectively. The delivery rate was 32%, and the miscarriage rate was 29% [43]. The cryopreservation of motile sperm using the novel SpermVD vector yielded improved clinical outcomes but immotile sperm were not preserved using this device, thereby limiting the knowledge regarding the efficacy of the SpermVD for immotile sperm from this source.…”

Section: The Novel Sperm Vitrification Device (Spermvd)mentioning

confidence: 99%

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Cryopreservation of single-sperm: where are we today?

2020

Reprod Biol Endocrinol

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Background: Patients with severe oligospermia and nonobstructive azoospermia have very limited numbers of viable sperm in their epididymal and testicular samples. Thus, cryopreservation of their sperm is performed to avoid repeated sperm retrievals and to preserve their sperm from any side effects of any treatment regimens. Main body: The development of intracytoplasmic sperm injection technology has extended the therapeutic capacity of assisted reproductive technology for men with azoospermia via the surgical or percutaneous isolation of sperm from the testis/epididymis. The conventional cryopreservation techniques are inadequate for preserving individually selected sperm. The technique for freezing single sperm was first developed in 1997 and has been explored from the perspective of frozen carriers, freezing programs, and cryoprotectant formulations. Among these methods, advances in frozen carriers have directly improved single-sperm freezing technology. In this review, we evaluate the different technologies for the cryopreservation of single sperm by discussing the advantages and disadvantages of different freezing methods, their clinical applications, and the outcomes for a range of frozen carriers. Conclusion: Our review article describes the latest and current technologies implemented for the cryopreservation of single sperm that could potentially benefit patients with severe oligospermia and who rarely have any sperm in their ejaculate. This review provides a platform to understand the process and pitfalls of single-sperm cryopreservation to ensure further improvements in the cryopreservation technology in future studies.

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“…The technique has already been widely explored and found its uses on embryos and oocytes and lately also for spermatozoa. [16][17][18][19][20][21]. Different studies have described individual protocols using different carriers and methods for standardization of the vitrification procedure.…”

Section: Techniques With Modified Protocolsmentioning

confidence: 99%

Sperm Cryopreservation: Principles and Biology

2020

J. Infertil. Reprod. Biol.

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Cryopreservation is a widely used method to preserve sperm prior to any cytotoxic therapy or testicular surgical intervention to be used later for assisted conception. Despite of multiple modifications in cryopreservation protocols, the yield of post-thaw good quality sperm has not been improved much. There is little data regarding factors affecting cryopreservation techniques and outcomes. Present review focuses on the basic biology of cryopreservation, its current protocols and effects on sperm proteomic and epigenomic modifications.

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A novel solution for freezing small numbers of spermatozoa using a sperm vitrification device

Cited by 55 publications

References 18 publications

Human sperm vitrification: the state of the art

Human sperm vitrification: the state of the art

Cryopreservation of single-sperm: where are we today?

Sperm Cryopreservation: Principles and Biology

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