A novel solid phase extraction – Ultra high performance liquid chromatography–tandem mass spectrometry method for the quantification of ochratoxin A in red wines

Mariño-Repizo, Leonardo; Kero, Frank; Vandell, Victor E.; Senior, Adam; Sanz-Ferramola, M. Isabel; Cerutti, Soledad; Raba, Julio

doi:10.1016/j.foodchem.2014.09.094

Cited by 41 publications

(22 citation statements)

References 28 publications

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“…No signal enhancement was observed, although there was a response reduction of approximately 95% as a result of matrix interference for red wine without applying any sample treatment procedure. As already noted, for the already reported SPE‐based methodology developed by our group, 20% of the matrix effect was still observed . Thus, to reduce the effect of the components in the matrix of the wine samples and to improve the OTA signal, the QuEChERS‐SPE approach proposed in the present study was developed.…”

Section: Resultsmentioning

confidence: 86%

“…As a consequence, a second clean‐up approach for removing interferents based on using SPE was evaluated to improve the OTA response. As noted, the SPE procedure was developed previously by our group . However, some modifications were required to be able to hyphenate QuEChERS to SPE and, consequently, to favor the retention/elution of OTA into the packed sorbent of the cartridge.…”

Section: Resultsmentioning

confidence: 99%

“…This procedure was shown to be of crucial importance for maximizing the sensitivity of OTA and minimizing the presence of interfering compounds in the extract. The conditions of QuEChERS and SPE procedure applied were developed previously by our group . The optimized conditions are provided in Table .…”

Section: Methodsmentioning

confidence: 99%

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Assessment of ochratoxin A occurrence in Argentine red wines using a novel sensitive quechers‐solid phase extraction approach prior to ultra high performance liquid chromatography‐tandem mass spectrometry methodology

Mariño-Repizo

Gargantini²,

Manzano³

et al. 2016

J Sci Food Agric

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Section: Resultsmentioning

confidence: 86%

Section: Resultsmentioning

confidence: 99%

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Assessment of ochratoxin A occurrence in Argentine red wines using a novel sensitive quechers‐solid phase extraction approach prior to ultra high performance liquid chromatography‐tandem mass spectrometry methodology

Mariño-Repizo

Gargantini²,

Manzano³

et al. 2016

J Sci Food Agric

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“…1 In great variety of foods, some species of Aspergillus fungi produce a carcinogenic metabolite called ochratoxin A (OTA). 2 Ochratoxin A is a stable molecule that resists degradation in acidic conditions, food processing and also blood serum with 35 days half-life. It can cause nephrotoxic, hepatotoxic, neurotoxic, teratogenic and immunotoxic diseases in humans.…”

Section: Introductionmentioning

confidence: 99%

“…[1][2][3][4] Several methods for laboratory analysis and detection of ochratoxin A in food samples such as thin layer chromatography (TLC), high-performance liquid chromatography (HPLC), gas chromatographymass spectrometry (GC-MS), fluorescence polarization immunoassays, capillary electrophoresis (CE), enzymelinked immunosorbent assay (ELISA) and fluorescent and surface plasmon resonance immunosensors have been reported. 1,2,5 The most frequently used method for the determination of OTA in grains and many other foodstuffs is extraction and clean up via immunoaffinity column (IAC) before employing the HPLC. [6][7][8] In all validated methods for the detection of OTA based on IAC, solvents were utilized for extraction of OTA from real samples.…”

Section: Introductionmentioning

confidence: 99%

A Simple and Fast Method for Magnetic Solid Phase Extraction of Ochratoxin A

Sargazi¹,

Aliabadi²,

Rahdari³

et al. 2016

J. Braz. Chem. Soc.

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We report on the synthesis of dopamine loaded magnetic nanoparticles (MNPs) for new, simple, fast and repeatable extraction of ochratoxin A from different solvents and milk without utilizing immunoaffinity columns and even high-tech devices. To this end, Fe 3 O 4 nanoparticles (NPs) were synthesized using thermal decomposition reaction and dopamine (DPA) was then conjugated with Fe 3 O 4 nanoparticles (NPs) to form Fe 3 O 4 -DPA NPs. Dynamic light scattering, field emission scanning electron microscopy and transmission electron microscopy revealed an average size of about 15 nm for Fe 3 O 4 -DPA NPs. Moreover, zeta potential measurement and vibrating sample magnetometer confirmed positively charged (16.8 mV) and superparamagnetic behavior of MNPs, which are effective factors for a good adsorbent in the extraction. Various solvents and different effective parameters were measured until acetonitrile:methanol was selected as the best extraction solvent. In addition, based on the pH-partition theory, with changes in pH, we were able to increase and enhance the extraction rate to 94%. Moreover, the ability of Fe 3 O 4 -DPA NPs in solid phase extraction of ochratoxin A from spiked milk was evaluated. The recovery rate for extraction of OTA from milk was 68%.

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Organometallic Au(III) Based Electrochemical Sensor with Wide Anodic Potential Window for Sensitive and Selective Detection of Ochratoxin A

et al. 2021

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Herein, an organometallic Au(III) catalyst was easily prepared via self‐assembly method using chloroauric acid (HAuCl4) and cetyltrimethylammonium bromide (CTAB). The electrode fabricated by the catalyst not only shows extended anodic potential window compared to that of Au nanoparticle‐based electrode, but also possess the high electrocatalytic activity in the selective oxidation of Ochratoxin A. Under optimized conditions, the modified electrode exhibits a wide linearity range from 1.0×10−7 to 1.0×10−5 mol/L and a low detection limit of 2.9×10−8 mol/L (S/N=3). Furthermore, the electrochemical sensor possesses good recoveries between 93.5 % and 98.2 % in real sample analysis, indicating high accuracy in real sample analysis.

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A novel solid phase extraction – Ultra high performance liquid chromatography–tandem mass spectrometry method for the quantification of ochratoxin A in red wines

Cited by 41 publications

References 28 publications

Assessment of ochratoxin A occurrence in Argentine red wines using a novel sensitive quechers‐solid phase extraction approach prior to ultra high performance liquid chromatography‐tandem mass spectrometry methodology

Assessment of ochratoxin A occurrence in Argentine red wines using a novel sensitive quechers‐solid phase extraction approach prior to ultra high performance liquid chromatography‐tandem mass spectrometry methodology

A Simple and Fast Method for Magnetic Solid Phase Extraction of Ochratoxin A

Organometallic Au(III) Based Electrochemical Sensor with Wide Anodic Potential Window for Sensitive and Selective Detection of Ochratoxin A

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