A novel signature-tagged mutagenesis system for Streptococcus suis serotype 2

Sialylation of Streptococcus suis serotype 2 is essential for capsule expression but is not responsible for the main capsular epitope

“…Results obtained imply that loss of sialylation might be related to the loss of CPS at the bacterial surface in S. suis. A neuB mutant of S. suis had been previously obtained by transposon mutagenesis and shown to be non-virulent in pigs [43]. Interestingly, we observed that this mutant was untypable with antiserum against S. suis serotype 2 and non-encapsulated by electron microscopy (unpublished observations).…”

Use of Selective Capture of Transcribed Sequences To Identify Genes Preferentially Expressed byStreptococcus suisupon Interaction with Porcine Brain Microvascular Endothelial Cells

“…While this work was under revision, an article was published (44) describing the use of the signature-tagged mutagenesis approach to study genes important for the virulence of S. suis in a pig model of infection. The ssu0457 gene reported in the present article was also found in that study.…”

Streptococcus suisinfection

“…So far, almost 60 bacterial components have been identified to be involved in the infection and/or pathogenicity of S. suis (Table 2). Of particular note, Wilson and coworkers 100 developed a powerful signature-tagged mutagenesis (STM) system for S. suis and identified nearly 20 potential virulence associate elements through screening the library consisting of approximately 2600 mutants (Table 2). However, exact roles of these genes needed further verification.…”

“…Among them, eight enzymes are proposed to be virulence factors by Wilson et al 100 using the system of signature-tagged mutagenesis (Table 2). Four of them are generally regarded as enzymes of central metabolism, which separately correspond to (1) GlnA, glutamine synthetase, 127 (2) Gdh, glutamate dehydrogenase, 128 (3) enolase catalyzing dehydration of 2-phosphoglycerate to phosphor-enolpyruvate, 96 , 129 , 130 and (4) Impdh, inosine 5-monophosphate dehydrogenase 131 .…”