A novel signal amplification tag to develop rapid and sensitive aptamer-based biosensors

Radfar, Sasan; Ghanbari, Reza; Isfahani, Ali Attaripour; Rezaei, Hadi; Kheirollahi, Majid

doi:10.1016/j.bioelechem.2022.108087

Cited by 6 publications

(5 citation statements)

References 68 publications

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“…In both cases, despite the improved capabilities, this analysis seemed to necessitate more time than the optimal duration expected for an ideal biosensor. Nevertheless, Radfar et al addressed this challenge in 2022 by employing a combination of CHA-HCR and RAFT polymerization for signal amplification [74]. This innovative approach significantly enhanced the sensitivity of miR-106a detection and obtained a LoD of 0.68 aM.…”

Section: Rna Nanobiosensors In Gcmentioning

confidence: 99%

Can Nano Yield Big Insights? Oligonucleotide-Based Biosensors in Early Diagnosis of Gastric Cancer

Avanu,

Ciubotariu,

Dodi

2024

Chemosensors

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Gastric cancer (GC) remains a significant global health challenge, with late-stage diagnosis impacting treatment options and decreased survival rates. To address this, there has been a growing interest in the development of innovative screening and diagnostic methods. Over the past 20 years, nanobiosensors have undergone multiple iterations and unveiled remarkable features that pledge to reshape patient care. Despite the excitement over the plethora of ground-breaking advancements for cancer detection, use-ready samples and streamlined healthcare information monitoring and usage, this technology is still awaiting entry into clinical trials, urging a closer gaze within the medical community. Oligonucleotide-based biosensors, leveraging DNA or RNA’s long-term storage of information, offer great specificity and sensitivity, as described throughout this paper. Consequently, this renders them as an ideal choice for revolutionizing GC diagnosis and facilitating early intervention. The aim of this review is to provide an overview of this cutting-edge, invaluable technology and its limitations across various aspects.

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Section: Rna Nanobiosensors In Gcmentioning

confidence: 99%

Can Nano Yield Big Insights? Oligonucleotide-Based Biosensors in Early Diagnosis of Gastric Cancer

Avanu,

Ciubotariu,

Dodi

2024

Chemosensors

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“…This aptasensor could accurately monitor miRNA-106a in real serum samples for gastric cancer diagnosis. 69…”

Section: Aptamer-based Diagnosismentioning

confidence: 99%

Aptamer-based technology for gastric cancer theranostics

Cui

Song

2023

Anal. Methods

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“…Nucleic acid molecules can be folded into a specific three-dimensional spatial structure. Apt can bind to the target molecule with high specificity and high affinity through the spatial structure matching and molecular interaction force between it and the target molecule ( 6 ). Compared with other biorecognition molecules, such as antibodies, Apt has a higher specific affinity and can be synthesized in large quantities in vitro by polymerase chain reaction (PCR) amplification technology.…”

Section: Introductionmentioning

confidence: 99%

MXene catalytic amplification-fluorescence/absorption dimode aptamer sensor for the detection of trace Pb2+ in milk

Zhi

Wei²,

Zhang³

et al. 2022

Front. Nutr.

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Lead ion (Pb2+) is a toxic heavy metal, which is very harmful to organisms. Therefore, the establishment of a rapid, simple, and sensitive method is of great significance to food safety and human health. It was found that MXeneTi3C2 nanosheet (NS) has a strong catalytic effect on the oxidation of 3,3,5,5-tetramethylbenzidine (TMB) via H2O2 to form the oxidized product (TMBOX); it has a strong fluorescence peak at 415 nm and an absorption (Abs) peak at 295 nm. The aptamer of Pb2+ (Aptpb) can be adsorbed on the surface of an NS to form MXene-Apt conjugates, which reduces its catalytic active sites and inhibits its catalytic activity. When the target Pb2+ is added, it specifically binds with Aptpb to release MXene NSs to enhance the dimode signals. Therefore, a new MXene catalytic fluorescence/absorption dimode aptamer biosenering platform was fabricated for the determination of trace Pb2+ in milk and water samples, with the fluorescence assay linear range (LR) of 5.0 × 10−2-2.0 nmol/L.

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A novel signal amplification tag to develop rapid and sensitive aptamer-based biosensors

Cited by 6 publications

References 68 publications

Can Nano Yield Big Insights? Oligonucleotide-Based Biosensors in Early Diagnosis of Gastric Cancer

Can Nano Yield Big Insights? Oligonucleotide-Based Biosensors in Early Diagnosis of Gastric Cancer

Aptamer-based technology for gastric cancer theranostics

MXene catalytic amplification-fluorescence/absorption dimode aptamer sensor for the detection of trace Pb2+ in milk

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